Process for detecting and typing all known genital tract human papillomavirus

Abstract

This invention discloses a method of quickly distinguish and classify existing genital meatus human papilloma virus. The method can distinguish existing 48 type HPV that can infect genital meatus mucous membrane and its normal mixed infection. And it can select Rsa I,Mse I,Pst I and HaeIII four normal using inscribing enzyme combinations according to optimal selection, optimize assort and at the same time the sensitivity and specificity can be ensured. Then HPV gene type is judged according to the established genital HPV gene type MY09 / 11 amplification product RMPH enzyme inscribing data. The operation is simple and time can be saved, the cost is low, its equipment needed exists in county hospital, and its cost is very low. It can be high pass quantity experienced, the technical operability of developing HPV inflection epidemiology investigation.

Description

technical field [0001] The invention relates to a method for detecting human papillomavirus of reproductive tract, in particular to a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism analysis) capable of detecting and typing almost all known human papillomaviruses of reproductive tract )method. Background technique [0002] Cervical cancer is the most common reproductive tract malignancy in women, and it has been confirmed that human papillomavirus (HPV) is the key pathogenic factor of cervical cancer and its precursor lesions. More than 200 types of HPV genotypes have been found, of which about 48 types are related to genital tract mucosal lesions. Since different reproductive tract mucosal HPV genotypes have different biological characteristics and different risks, it is necessary to detect and type HPV at the same time; at the same time, with the development of vaccine research, a good HPV typing method should be established It is very import...

AI Technical Summary

Problems solved by technology

Since different reproductive tract mucosal HPV genotypes have different biological characteristics and different risks, it is necessary to detect and type HPV at the same time; at the same time, with the development of vaccine research, a good HPV typing method should be established It is very important for the targeted use of vaccines, but so far, there is no suitable method to detect and type all HPVs that are currently known to infect the genital mucosa

In recent years, there have been many studies on papillomavirus typing, but some methods have low sensitivity, such as Southern blotting and in situ hybridization; some methods can only simply determine high-risk and low-risk types but cannot Typing is like the hybridization capture method; although some are the most accurate for the detection of a single genotype, they cannot be judged when mixing samples, such as sequencing; Technology costs are very expensive, such as DNA chips

The existing restriction fragment polymorphism (RFLP) method can only detect a few limited types of HPV, such as HPV16, 18 and 6, 11

[0003] In summary, so far, there is no method that can meet the requirements of sensitivity and specificity and can detect all known genital tract mucosal HPV types

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