The invention relates to a deer epidemic hemorrhage competitive
enzyme-linked immunosorbent
assay kit and a preparation method and a user thereof, the kit comprises an EHDV
antigen-coated ELISA plate,a
monoclonal antibody IgG-HRP
enzyme conjugate, positive serum, negative serum, 20 times of concentrated washing liquid, 10 times of concentrated
dilution buffer, a substrate I, a substrate II, a substrate III and stop solution. The preparation method comprises the following steps: a, preparing a deer epidemic hemorrhage
virus ELISA-coated
antigen, and detecting the safety of the deer epidemic hemorrhage
virus ELISA-coated
antigen; b, preparing a
monoclonal antibody of deer epidemic hemorrhage
virus and carrying out preparation and identification on the
antibody-
horseradish peroxidase (HRP)
enzyme conjugate; c, preparing the positive serum and the negative serum; d, preparing the deer epidemic hemorrhage
virus antigen-coated ELISA plate; e, preparing the 20 times of the concentrated washing liquid and the 10 times of the concentrated
dilution buffer, and preparing the substrate I, the substrate II, the substrate III and the stop solution; and f, assembling the kit. The kit is used fordiagnosis,
quarantine, detection and epidemiological investigation of deer epidemic hemorrhage and is characterized by strong specificity, high sensitivity and the like.