ELISA detection method and reagent kit for bluetongue viral antigen

Abstract

The invention discloses an ELISA detection method and a kit of blue-tongue virus antigen. The detection method uses the monoclonal antibody of anti-blue-tongue virus VP7 protein as dual-anti sandwich ELISA detection method of a coating and enzyme-marked antibody; and the kit comprises the monoclonal antibody of the anti-blue-tongue virus VP7 protein of the coating and enzyme-marked antibody. The kit and the detection method can be used for detecting the blue-tongue virus antigen, have higher specificity and sensitiveness, can be used in large-scale forserological detection, is suitable for epidemiological investigation and has wide application prospect.

Description

technical field [0001] The invention relates to a double-antibody sandwich ELISA detection method and a kit for bluetongue virus (BTV) antigen in serum (plasma). Background technique [0002] Bluetongue disease (BT) is caused by bluetongue virus (BTV) and is a viral infectious disease of ruminants transmitted by Culicoides. The World Organization for Animal Health (OIE) lists it as a reported animal disease (OIE Terrestrial animal health code, 13th ed.2004. Terrestrial Animal Health Code. Thirteenth Edition, Office International des Epizooties, Paris.), and is listed as a class of animal in my country Diseases (http: / / www.ivdc.gov.cn / ). The incubation period of infected animals is generally 5 to 12 days, and the course of disease is generally 6 to 14 days. The average morbidity rate of ruminants after infection is 30-40%, and the mortality rate is 20-30%, among which the mortality rate of sheep is 80%-90%. [0003] In recent years, outbreaks of bluetongue have occurred all...

AI Technical Summary

Problems solved by technology

There have been many related studies in China, but no commercialized BTV detection kits have been approved for marketing. Only fluorescent antibody reagents have been seen abroad, but they are expensive and difficult to obtain. Commercialized BTV antigen detection kits and BTV nucleic acid detection reagents The box has not been listed at home or abroad