414 results about "Incubation period" patented technology

The present invention relates to a method and apparatus for forming a thin film using the ALD process. Prior to the ALD process where each of a plurality of source gasses is supplied one by one, plural times, a pretreatment process is performed in which the source gasses are simultaneously supplied to shorten an incubation period and improve throughput.

Embodiments include determining attributes of a property using an incubation pool. An incubation period is identified based on estimated attributes for the property, and based on rules that define a minimum and a maximum incubation time. The property is added to the incubation pool, and load and patterns for the property are analyzed. A load size and / or a traffic type for the property is identified based on the incubation. Embodiments also include offloading traffic within a CDN. A load balancer agent determines that traffic at an edge data center should be offloaded to the other edge data centers. The load balancer agent sends a request, including a determined priority level. The load balancer agent receives one or more replies indicating that resources are available for use. The load balancer agent sorts the replies and offloads traffic to at least one edge data center.

A system and method of automated incubating and reading of biological indicators is disclosed. In one embodiment, the automated biological indicator incubator is configured to control the temperature of incubation test wells to a desired temperature range suitable for use with a biological indicator, control the incubation period, and detect a change in the biological indicator colored media providing an indication of growth or lack of growth. In other embodiments, the automated biological indicator incubator is self-calibrating, and provides a communication interface to an external device, such as a computer. The communication interface is suitable for use to collect and analyze data associated with the biological indicator during the incubator period, and for making a determination of growth or lack of growth and success of the sterilization process as well as a permanent document of the monitoring of the sterilization process.

The present invention provides methods for the determination of the viral titer of a culture of host animal host cells infected with a circovirus. The FACS-based methods of the invention may include determining the viability of the host cells in a cell culture medium supernatant and of those cells that remain adherent to a solid support. Detecting and measuring the percentage of cells that expressed the viral antigens ORF1 and ORF2 may determine the viral load of the cultured host cells. The yield of the virus may be established by the detection and measurement of both antigens in supernatant cells, for example 5 to 7 days from when the host cells are transferred to a serum free medium. The methods of the invention may yield rapid quantitative data. This allows the repeated in-process monitoring of the viral production throughout the incubation period, and ready selection of the most appropriate harvesting point.

The invention relates to a production method for an environment-friendly soilless lawn carpet, which mainly comprises the following steps: laying a waterproof layer, nonwoven fabric, a wadding fiber layer and a nutrition substrate layer in sequence; seeding grass seeds, coating with the nonwoven fabric and then laying vermiculite or zeolite particles; and finally periodically watering and fertilizing during the incubation period. The lawn carpet produced by the invention has short lawn forming time, labor conservation, low labor intensity, low management cost, high lawn purity, no weed, complete root system, good grass quality, uniform thickness, easy survival after transplantation, convenient management, and reeling and cutting property, and overcomes the defects of the prior lawn such as bad applicability and easy rupture. The produced lawn carpet has the advantages of long green period, fresh and green color, strong resistance, light mass, degradability, convenient maintenance convenient transportation and the like, and can be widely applied to the afforestation or the decoration of public places, airports, office buildings, residential building roofs and the like.

A method of determining the viability of an egg at least approximately 50% through its incubation period, which method comprises the steps of: (a) causing electromagnetic radiation to impinge upon the egg, the electromagnetic radiation having one or more wavelengths in the infra-red part of the spectrum; (b) receiving at least a part of the infra-red radiation that has passed through the egg and generating an output signal representative of the received infra-red radiation; and (c) processing said output signal to determine whether there is a cyclical variation in the intensity of the infra-red radiation leaving the egg corresponding to action of a heart, the existence of said cyclical variation indicating that the egg is viable; wherein step (a) is performed by directing infra-red radiation so that it passes through the shell for reflection from an outer surface of a vascular structure adjacent an inner surface of said shell, and step (b) is performed by receiving any infra-red radiation so reflected.

A system for lead incubation is used to alert an agent and to customize contacts with leads generated by an internet form, return mail form, voicemail, or telephone inquiry. For example, a lead may be interested in purchasing real estate, which requires a long lead incubation period. The system dynamically and recursively classifies the lead according to actions taken by the lead, requests made by the lead, and interactions between the lead and the system and / or agent. Based on heuristic patterns and triggers, the system provides suggestions to the agent for marketing campaigns for a lead or a class of leads, and alerts the agent when action or contact between the agent and the lead is recommended. The system is customizable by lead, by agent performance and / or by class of lead.

Processes and compositions for liposomal delivery of therapeuticals prepared by contacting an aqueous solution of an active agent with a solution of liposome-forming components containing one or more DILA2 amino acid compounds or lipids in organic solvent to form an impinging stream. A protocol including flow rates, pH, and an incubation period are used to control formation of liposomal components for therapeutic applications. The impinging stream may be collected and incubated to prepare a liposomal formulation which encapsulates the active agent. The composition can be quenched with buffer and filtered by tangential flow and diafiltration and other means for finishing as a pharmaceutical composition. An efficiency for delivering a drug cargo is provided. Compositions can include a liposome containing one or more carrier particles, each carrier particle having an active agent and a peptide, wherein the ratio of the mass of the peptide plus the mass of the liposome to the mass of the active agent is less than about 15.

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

A system and method for developing and utilizing particle-based n-multiplexed assays that include three or more reporters utilizes n particle sets that are associated with particle identification images or labels (IDs) that differ between sets. The encoded particles for a given set are coated with a specific binding member, or in the case of the sandwich assay with coupled capture and detector binding pair members, to form particle types. The sets of particle types are then pooled, and aliquots of the particle types are removed to assay vessels. Next, samples with three or four reporter molecules are supplied to the respective vessels. After one or more incubation periods, the particles are supplied to a reader system, which determines the particle IDs to identify the particle types and also detects the reporter signals. The reader system includes multiple excitation lasers that excite the various reporters in sequence or in parallel, to supply associated signals to one or more detectors. Emission filters and wavelength discriminators are included such that a given detector receives at a given time the signals associated with a single assay binding label. The system further develops greater capacity sandwich assays by assigning subsets of capture and detector antibody pairings to the three or four reporters, respectively. The system performs greater numbers of differential RNA expressions based on the use of the three or more reporters, with one or more reporters assigned to the reference sample and the other reporters assigned to respective test samples. The system and method are also capable of performing greater numbers of SNPs utilizing primer extension reactions, by assigning different color reporters to the respective nucleotides or terminators.

In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

The invention discloses a remote sensing technology-based grassland locust plague progressive prediction method. According to the method, the distribution of key habitat elements which influencing the development of a grassland locust population is obtained by the means of quantitative remote sensing inversion, meteorological station observation and the like, wherein the key habitat elements subjected to remote sensing inversion comprises land surface temperature, vegetation coverage and soil moisture; the spawning suitability, the incubation suitability and the growth suitability of locusts are analyzed quantitatively by establishing an evaluation model, and a locust plague risk early prediction model is established; a locust plague risk level prediction result is corrected by utilizing the remote sensing observation of an incubation period and a third period and the locust density data measured in the field according to the incubation and development time axes of the locusts, and the situations of a grassland locust plague are predicted progressively. According to the technical scheme provided by the invention, the progressive update of sensitive habitat elements is obtained by performing quantitative inversion on remote sensing data with higher time resolution, so that the prediction precision of a locust plague monitoring and prediction model is improved.

System and methods to perform inhibition diagnostic testing utilizing a Petri dish based packaged applied materials kit to conduct growth inhibition testing of essential oil compositions, herbs, antibiotics, antifungals, anti-parasititic compounds applied to fungi, bacteria or other biological materials having confluent growth patterns that are derived from a patient's inoculum grown in a personal incubator. Resulting inhibition patterns are generated by at least one essential oil and / or a panel of essential oils or other applied materials distributed in fixed concentrations or concentration gradients to confluent lawns of bacterial and / or fungal lawns derived from the patient's clinical specimen. Images are acquired during the incubation periods to measure inhibition areas or zones that developed around applied the applied material compositions and the optimal therapeutic treatment is ascertained from analysis of the developing or generated inhibition zones.

The invention relates to infectious disease epidemic situation prediction and belongs to the field of epidemic disease spreading and control. According to an infectious disease with an immune period, the invention provides an epidemic situation prediction method for the infectious disease having the immune period and influenced by seasons. Compared with the existing models, the method comprises the following steps: considering the influence of the immune period, setting up an epidemic situation prediction dynamic model for the infectious disease having the immune period and influenced by the seasons according to the change rate of relative time of susceptible persons, persons in an incubation period, infected persons and recovered persons, substituting an infection rate, the immune period, the incubation period and the like to obtain the change rules relative to time of all kinds of crowds, and completing the epidemic situation prediction of the communicable diseases. The method has higher prediction accuracy, and provides more reliable evidences for the epidemic situation control.

Methods of treating a subterranean formation penetrated by a wellbore of a well, wherein the subterranean formation includes carbonate. The methods can include the following steps of: (1) optionally, fracturing the subterranean formation; (2) optionally, acidizing the subterranean formation with a Bronsted-Lowry acid; (3) treating the subterranean formation with an acid-producing microorganism, a nutrient for the microorganism, and, if needed, a suitable electron acceptor for respiration by the microorganism; (4) optionally, flushing the wellbore with a wash fluid to push the microorganism deeper into the subterranean formation and wash it away from the metal tubulars of the well; (5) preferably, shutting-in the well for a required incubation period for in-situ acid generation by the microorganism; and (6) preferably, after the shut-in, flowing back fluid from the subterranean formation into the wellbore.

A method for operating a measuring meter for an electrochemical test strip is provided. The method comprises steps of applying a voltage to electrodes of the test strip at least during one of a sample presence detection period and an incubation period; and repeatedly increasing and decreasing the voltage during at least one of the sample presence detection period and the incubation period.

The invention discloses a coal mine water burst down-hole full-space real-time continuous monitoring early-warning method which comprises the steps that 1. a group of slight shock detectors are arranged on a coal mine down-hole excavating working face or near a construction weak zone, and all slight shock events in a certain time period in a monitoring zone are subjected to full-space real-time continuous monitoring; 2. all the slight shock events are subjected to time locating and space locating; and 3. according to a space profile distribution graph in the step 2, a slight shock event dense distribution zone in a water source layer is subjected to incubation period blue early warning, a slight shock event dense distribution zone in a water insulating layer is subjected to development period yellow early warning, and a slight shock event dense distribution zone near the excavating working face is subjected to burst period red early warning. The method can carry out omnibearing real-time continuous monitoring and effective early warning on a water source layer, a water insulating layer and an excavating layer, so that real-time judging of water burst time, the water burst point position and the water burst type is achieved, and significance is achieved on reducing and avoiding of water burst accidents and quick treating of water burst.

The invention provides an intelligent system for health diagnosis and treatment, which comprises a computer, an input device, an output device, a data acquisition device and a database, wherein the database is arranged in the computer; the data acquisition device is connected with the computer through the input device; the data acquisition device acquires meridian point electrical information, tongue shape images, shape signs, breath, touch and question-feedback information of diagnosed-treated objects; the computer is provided with a processing program run according to the principles and rules of Chinese medicine; and the processing program compares acquired information with corresponding information in the database, obtains the results of the status characteristics of the constitution ofhuman bodies, and outputs the results through the output device. The system solves the technical problem that the prior art is difficult to arrange, process and comprehensively analyze the relevant information of the human bodies in 'not sick', 'to be sick' and 'disease incubation period' stages.

A photostimulation device of decision behaviors comprises a central control system, a barrier maze system, a photostimulation system, an infrared detection system and a video analysis system, wherein the central control system is respectively connected with the photostimulation system, the infrared detection system and the video analysis system and is used for controlling the photostimulation system, the infrared detection system and the video analysis system, the photostimulation system is used for applying photostimulation signals to animals to be tested, the infrared detection system is used for monitoring the moving process of the animals to be tested in the barrier maze system, and the video analysis system is used for recording and analyzing the moving state of the animals to be tested in the barrier maze system. According to the device, a reward-system-based and effort-based decision behavior testing model is established, the states of the animals before and after the choice and the reaction incubation period are judged, and people can have interference in the decision behaviors of the animals by combining optogenetics method.

The clinical analyzer includes a cuvette carrier that is moved in a manner to provide flexible assay timing and variable incubation periods. Multiple assays having such varied incubation times can be run concurrently in random-access, avoiding timing conflicts. Fluid delivery stations are placed around the cuvette carrier in positions that are independent of assay timing. The cuvettes move in unison, in multiples of incremental steps, along a closed geometrical path. The cuvette carrier is movable variable distances in opposite directions in a single time cycle to position specific cuvettes at specific locations for delivery of sample or reagent. The direction of movement of the cuvette carrier is preferably based on a determination of the shortest distance between the cuvette and respective fluid delivery stations. However, in each time cycle there is a net progressive incremental stepwise movement of the cuvettes in a selected direction.

A nutrient-germinant composition to aid in spore germination and a method for increased spore germination efficiency. The composition comprises L-amino acids, D-glucose and / or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores or they may be separately combined for germination. The method comprises providing a nutrient-germinant composition and bacteria spores, preferably of one or more Bacillus species, and heating to a preferred elevated temperature range of 41° C. to 44° C. for an incubation period of around 2 to 60 minutes. The nutrient-germinant composition is preferably in a concentrated liquid form that is diluted just prior to initiating the germination / incubation method at the point of use. The method may also include dispensing a germinated spore solution to a point-of-use / consumption, such as animal feed, water, or bedding, or a wastewater system or drain.

The invention belongs to the metallurgical field and particularly relates to a permanent modifier capable of improving electrical conductivity of a hypo eutectic Al-Si alloy as well as a preparation method and a using method of the permanent modifier. The permanent modifier comprises the following components in percentage by weight: 1-4% of Sr, 5-20% of lanthanum-cerium-rich mixed rear earth and the balance of Al, wherein a weight ratio of the Sr to the lanthanum-cerium-rich mixed rear earth is 1 to (5-10). The preparation method for the permanent modifier comprises the following steps: firstly, preparing materials and smelting the prepared materials; and then, carrying out alloying and carrying out thermal extrusion by virtue of billets prepared by extruding and casting to prepare a modifier wire, thus obtaining the permanent modifier product capable of improving electrical conductivity of the hypo eutectic Al-Si alloy, wherein effective modifying time is at least 5 hours. The permanent modifier disclosed by the invention is convenient to use, stable in effect, long in effective modifying time, free of a modifying incubation period, and has effective modifying time of at least 5 hours according to detection. And moreover, the electrical conductivity of the alloy treated by virtue of the modifier wire is obviously improved.

The invention discloses an IF steel cover type annealing technology, which is characterized in that a way of staged thermal insulation is adopted in the annealing process. Through the way, crystal grains have enough incubation period in the nucleation process, and the nucleation rate N of a re-crystallization can be improved. Due to the inverse proportion relationship between the size of the crystal grain of the re-crystallization and the nucleation rate of the re-crystallization, the nucleation rate N of the re-crystallization is improved, or the size of the crystal grain after the re-crystallization becomes small, so that the crystal grains are refined. Moreover, a gamma- structure is enhanced, and the second phase particles are small to be dispersed. The obtained IF steel products have excellent deep drawing property, thus solving the current difficulty that the IF steels with excellent deep drawing property are hard to be produced by the cover-type annealing furnace.

The invention discloses an intensified artificial breeding method for Australian crayfishes. The method comprises the following steps of: putting pubescent parent crayfishes which pull through a wintering period into a spawning pond according to a proportion of 1-3: 1 of female and male, wherein circulating water is adopted in the spawning pond to automatically control and increase oxygen; sterilizing fertilized ovigerous female crayfishes, putting into small hatching lattices in a hatching pond, wherein one crayfish is hatched in each small hatching lattice, and carrying out water-flow hatching; and enabling hatched young crayfish seeds to leave away from mother bodies after 30-50 days of incubation period, enabling the young crayfish seeds to escape into water through a filter screen of the hatching pond, after all the crayfish seeds at the abdomen of the ovigerous female crayfishes leave away from the mother bodies, immediately separating the parent crayfishes, and remaining the young crayfish seeds to be continuously cultivated for 5-10 days, so that the cultivation can be carried out. According to the method, the artificial intensified breeding incubation can be carried out according to the required offspring seed quantity and the required offspring seed time at any time, the water temperature, the dissolved oxygen, the water quality and the like can be controlled, the method can not be influenced by environment, and the method is high in fertilizing rate, spawning rate and hatching rate.

A cover member for a substrate supporting a biological sample comprises first and second opposing ends, first and second opposing surfaces, a void in the second surface which, when juxtaposed with a substrate, forms a chamber, and a fluid inlet toward the first end and in fluid communication with the void. The void is bounded by void walls having one or more contoured regions for enhancing fluid movement within the chamber. A treatment module for a biological sample comprises the cover member, a support surface for a substrate bearing the biological sample and clamp means operable to releasably retain the cover member in juxtaposition with the substrate for an incubation period. A method for incubating the biological sample with one or more reagents uses the cover member.

The invention provides an image quality evaluation method based on an EEG signal. The method comprises steps that a stimulus graph is respectively generated according change of color saturation, white brightness and sharpness of a reference graph; the reference graph and the stimulus graph are sequentially and continuously displayed to a testee, and an EEG signal of the testee is simultaneously acquired according to whether the testee can distinguish the reference graph from the stimulus graph; a P300 wave peak value and incubation period data are acquired according to the acquired EEG signal of the testee, an incubation period is defined to be time duration from occurrence of stimulus to occurrence of a peak value, and the unit of the incubation period is commonly ms; an incubation period and peak value prediction model of each stimulus graph is acquired according to the color saturation, white brightness and sharpness stimulus graphs of the reference graph.

The invention discloses a method for detecting a pathogenic microorganism by using antigen-stimulated cellular immune response. In the method, immune cells, which have been exposed to the microorganism, in blood are stimulated by specific antigen of the pathogenic microorganism or a functional segment of the pathogenic microorganism, so a cell factor, such as interferon gamma (IFN-gamma), is produced by the immune cells; and then whether the pathogenic microorganism exists in animals is judged by detecting the amount of the IFN-gamma. In the invention, the IFN-gamma in the blood is detected by a colloidal gold-labeled immunochromatographic assay. In addition, the invention also discloses a colloidal gold immunochromatographic assay test pen for detecting the pathogenic microorganism. By the method, the pathogenic microorganism in an incubation period or early morbidity can be detected quickly and effectively.

The invention relates to a method for realizing high-yield clown fish parent fish culture in running water, which comprises the following steps: classifying clown fishes from a productbility angle and respectively selecting suitable culture containers; selecting wild clown fish parent fishes or juvenile fishes for artificial pairing; strengthening the simulation of natural environments for egg laying of parent fishes, including the simulation of habitat, water flow and illumination; selecting different maturing baits for maturing the clown fishes according to the different types of clown fishes; and strengthening management during incubation period and reaching the effects of high egg laying amount and high hatching rate of the cultured clown fish parent fishes in a running water culture system. The invention solves the existing common problem that the egg laying amount and the hatching rate of the clown fish parent fishes are low, improves the situation that the culture yield of the clown fishes is low and is of great importance to the industrialized development of clown fish culture.

A suspended fish egg incubator is composed of a net incubation bucket and a suspended bracket which is used for hanging the net incubation bucket and suspensible on the water face, and an air cascade head is arranged at the bottom of the net incubation bucket. The invention has the beneficial effects that: the structure is simple and the manufacture cost is low; a micro air pump is used for replacing a water tower to supply water for stirring the fish eggs being incubated, the mounting and using are more convenient and the cost is low, and the water source pollution influence can be avoided; mounting and dismounting and carrying are convenient, and the using is not restrained by places; the invention is applicable to direct fish egg incubation in mountain reservoir, lakes and other natural waters as well as in indoor pools, the water temperature can be adjusted, the incubation period can be shortened, and the incubation rate can be improved; use and management are convenient and the labor intensity is light; the invention provides a simple, convenient, efficient and economic fish egg incubation device for the small-scale proliferation of young fishes and famous-brand high-quality marine young products.