77 results about "Coilia" patented technology

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

The invention relates to an indoor intensive artificial breeding technique of Yangtze River Coilia ectenes, which comprises the steps of Coilia ectenes parent fish enhanced breeding, artificial spawning induction, artificial hatching and fingerling breeding. The indoor intensive artificial breeding technique of Yangtze River Coilia ectenes is characterized in that Coilia ectenes adult fish which is alternately cultured for three years by using seawater and freshwater is used as parent fish for the Coilia ectenes parent fish enhanced breeding, female parent fish individuals with clearly visible ovary outlines and softer inferior bellies and male parent fish individuals with milk white semen which flows out when the bellies are slightly pressed are selected for the artificial spawning induction, the female-male ratio is 1:3, the female parent fish and the male parent fish naturally mate for laying eggs and fertilization, temperature-controlled inflated hatching in still water is conducted to fertilized eggs, and the fingerling breeding comprises culture at four stages, wherein the culture at the first stage comprises the steps of temporarily culturing newly hatched fries from the time that fries are newly hatched to the time that the fries open mouths, the culture at the second stage comprises the step of culturing the fingerlings from body length being 3mm to body length being 20mm, the culture at the third stage comprises the step of culturing the fingerlings from body length being 20mm to body length being 30mm, the culture at the fourth stage comprises the step of culturing the fingerlings from body length being 30mm to body length being 40-50mm, and the fingerlings can be put in a pond for conventional culture after the body length of the fingerlings reaches 40-50mm.

The invention discloses a coilia mystus Maillard peptide with a memory improvement function as well as a preparation method and an application of the coilia mystus Maillard peptide. The method comprises following steps: coilia mystus is minced into meat paste, water is added for mixing, protease is added for enzymolysis, and an obtained supernatant is a coilia mystus enzymatic hydrolysis solution; reducing sugar is added to the enzymatic hydrolysis solution and reacts at the temperature of 90-110 DEG C for 1-2 h, a product is taken out and cooled, and coilia mystus Maillard reaction solution is obtained; the coilia mystus Maillard reaction solution is subjected to vacuum concentration and spray drying, and the coilia mystus Maillard peptide is obtained. The biological enzymolysis technology is firstly adopted to release protein peptide with the memory improvement function in coilia mystus protein to the greatest extent, and then the biological efficacy of the protein peptide is further improved through the Maillard reaction. Meanwhile, the Maillard reaction can also improve the flavor of enzymatic hydrolysates, remove fishy smell of marine products and produce pleasant fragrance.

The invention provides an ecological factorial breeding method of coilia ectenes parent fish. The ecological factorial breeding method of the coilia ectenes parent fish comprises the following steps: 1) an ecological spawning pond is built; 2) parent fish is selected; 3) the ecological spawning pond is processed; 4) the ecological spawning pond is managed; 5) the breeding is managed. The ecological factorial breeding method of the coilia ectenes parent fish artificially simulates a Yangtze River coilia ectenes breeding migration rule, the parent is provided with an ecological factorial breeding migration environment, by artificially controlling the external conditions of water flow, water temperature, illumination intensity and a period, osmotic pressure and the like, the fresh food intensity is strengthened, the parent is enabled to develop from body maturity to sexual maturity, parent fish can be kept every year, and the sustainable development of coilia ectenes ecological factorial breeding is achieved.

The invention relates to an indoor cement pond culture technique of Yangtze River Coilia ectenes, belonging to the field of Coilia ectenes culture. The indoor cement pond culture technique of the Yangtze River Coilia ectenes comprises the steps of fingerling acquisition and transportation, temporary culture and domestication, indoor culture and overwintering culture. The indoor cement pond culture technique of the Yangtze River Coilia ectenes is characterized in that juvenile fish with body length being 8-12cm is acquired in September to October and is put in an indoor cement pond for temporary culture; after the juvenile fish is domesticated for 2-4 weeks, the juvenile fish is transferred into an indoor small cement pond for culture; when the juvenile fish is cultured to November to December of each year and the water temperature is below 15DEG C, the Coilia ectenes is transferred for the overwintering culture, the overwintering culture of the Coilia ectenes is conducted at room temperature and the water temperature is kept above 8DEG C to keep the juvenile fish to intake food; during overwintering, live seawater copepods are used as fish feed, and if the live fish feed is not enough, the live fish feed can be replaced by frozen opossum shrimps or slow-sinking compound feed; and after the spring of the next year begins, the juvenile fish is cultured according to the indoor culture method.

The invention provides a method for ecologically and early breeding Yangtze River coilia ectenes in a pond greenhouse, and relates to coilia ectenes cultivation. The method comprises production steps of parent overwintering and strengthening cultivation, breeding parent stocking, mysis inoculation, daily management, fish fry number estimation and parent removing. The method is characterized in that when the water temperature of an outer pond is reduced to 7-8 DEG C from December to January in the following year, healthy two-age coilia ectenes without surface damage is taken as parent fish and transferred to the pond greenhouse for overwintering and strengthening cultivation; after the parent fish enters the greenhouse, 3 kilograms of exopalaemon carinicauda are put into the pound for each mu, and the mysis is inoculated; from the end of March to the middle ten days of April, the water temperature rises to 15-16 DEG C, overwintered and strengthened parent fish is picked and transferred to an ecological breeding pond, growth and feeding conditions of the fish fry are checked every one week, and the density of fish bait in water is adjusted according to the checked conditions; a seine is used for removing the parent fish in the middle ten days and last ten days of June, 1, 000-2, 000 fish fry can be generally seined each mu, the fish fry specification is that the total length is 7-10 cm and the weight is 1.8-2.5 g, and the fish fry can be taken as culturing offspring seeds.

The invention discloses a brackish-water ecological cultivation technology for Yangtze-river two-year-old coilia ectenes fingerlings and exopalaemon carinicauda. The technology is characterized by comprising the following steps: firstly, an estuary-area earth pond and natural brackish water with an inflow salinity of 0.5-1.5% are chosen, and food organism is inoculated in the pond; secondly, an aerator is opened before fingerlings are put in the pond in winter or in early spring, wherein the stocking size of the fingerling is a total length of 10 to 20 cm, and the stocking density of the fingerlings is 500 to 600 fishes per mu; thirdly, putting the exopalaemon carinicauda into the pond, wherein the stocking density of the exopalaemon carinicauda is 6 to 12 jin per mu, and the stocking size is 200 to 280 fishes per jin; after the fingerlings and exopalaemon carinicauda are put in the pond, 1 / 3 of water is changed each month from January to June, 1 / 3 of water is changed each half month from July to September, and 1 / 3 of water is changed each month from October to December; fourthly, prawn artificial pellet feed which is adopted as exopalaemon carinicauda feed is fed 1 time per day, wherein the feeding amount is 1 to 3 percent of the weight of the exopalaemon carinicauda; finally, the fingerlings and exopalaemon carinicauda are captured with nets in winter at the temperature of 6 to 8 DEG C, wherein the specification of the captured coilia ectenes is 16 to 20 gram per fish while the specification of the exopalaemon carinicauda is 100 to 150 fishes per jin.

A coilia ectenes step type breeding method comprises the nine steps of reserved parent pool simulated ecological cultivation, parent pool intensive culture, parent selection for induced spawning, parent induced spawning, natural insemination, germ cell collection, germ cell incubation, indoor intensive fry rearing in a cement pool and large-scale fry culture in an outdoor pool. The method is characterized in that filial-generation coilia ectenes adult fishes which are more than 2 years old and cultured by manpower are used as reserved parents for artificial propagation, and a natural ecology cultivation mode is simulated; the selected parents are transported and placed in an induced spawning pool, after parent induced spawning is conducted, the germ cells are collected, counted and transferred to an incubation pool, after the germ cells are collected, the germ cells are placed in the incubation pool with water, fries open the mouths 5-6 days after membrane rupture is conducted, yolks and freshwater rotifers which are filtered through a 80-mesh silk net are fed to the fries, after the fries are cultivated for 15 days, the fries are divided to different pools, and when the fries are as long as 20-30 mm, the fries are placed to outdoor pools to be cultivated.

The invention relates to a transfer technique of indoor bred Yangtze River Coilia ectenes summer fingerlings, belonging to the field of live fish transportation. The transfer technique consists of steps of net hauling for exercise, net hauling operation and transportation. The transfer technique is characterized in that net hauling for exercise is conducted to the fingerlings before transportation; feeding is forbidden one day before transportation, net hauling operation is conducted to gather the fingerlings in a net enclosure, the fingerlings are scooped into a transportation tool by using a water ladle or a basin and the fingerlings are guaranteed not to leave water during operation; the transportation is divided into short-distance transportation and long-distance transportation according to transportation duration, the duration of short-distance transportation is controlled to be 30-40 minutes, containers for containing the fingerlings for short-distance transportation are plastic barrels, the quantity of fingerlings in each barrel is controlled to be 100-200 and the water temperature is controlled to be 28DEG C; and the duration of long-distance transportation is controlled to be 4-5 hours, the fingerlings in the net enclosure are scooped into a small net cage for temporary culture for 15-30 minutes by using the water ladle after net hauling is conducted, then the fingerlings are contained in bags for transportation, five liters of water and 50 fingerlings are contained in each bag, the bags which contain the fingerlings are contained in black thin film bags to prevent irritation of strong light, and the water temperature is controlled to be below 28DEG C.

The present invention discloses a method for researching age and migration life history of coilia by eardust which belongs to the ichthyological ecology field. The method selects the eardust as researching material, processes grinding to an intersecting surface of the eardust, observes a micro-structure of the eardust for researching age, growth and life history of the coilia. Compared with traditional method, the method provides a new method for researching fish type annual zone, fills blank of unclear bright band and dark lane on the eardust to research fish type age; the eardust grinding has high efficiency, each eardust can be ground only five minutes, and the result is more reliable than other hard bone tissue analyzing; a nail enamel has transparent function to the eardust in embedding and enveloping simultaneity which is more safety and has better transparent effect than using dimethylbenzene. The method is suitable for researching relative age and migration life history of all hard bone fish type with eardust back and belly morphology curve.

The invention discloses a method for cultivating fries of coilia ectenes in a soil pond, comprising the steps of selection of an outdoor soil pond fry cultivation pond, secondary intensified cultivation of parent fishes, separation of the parent fishes and the fries, and early-stage cultivation of the fries of the coilia ectenes by cultivating food organisms including rotifer, cladocera and the like. The method disclosed by the invention is simple and convenient to operate, economical and practical, realizes repeated use of the parent fishes, and is low in fry cultivation cost, high in fry survival rate, and strong in disease resistance. By adopting the principle of natural selection, weak and sick fries are eliminated, so that the purpose of improving the fry quality is achieved. In addition, pelagic organisms are cultivated by utilizing glucose, amino acid and other nutrient solutions, so as to provide sufficient baits for the fries, and thus the quick growth of the fries is guaranteed.

The invention discloses a pond polyculture method for Yangtze-river two-year-old coilia ectenes fingerlings and scatophagus argus. The method is characterized by comprising the following steps: firstly, an estuary-area pond and natural brackish water with an inflow salinity of 0.5-1.5% are chosen, and food organism is inoculated in the pond; secondly, fingerlings are put in the pond in winter or in early spring, wherein the stocking size of Yangtze-river two-year-old coilia ectenes fingerlings is a total length of 10 to 12 cm, and the stocking density thereof is 500 to 600 fishes per mu; thirdly, the scatophagus argus is put in the pond from the end of April to the beginning of May, wherein the stocking density is 150 to 250 fishes per mu,and the stocking size is 20 to 30 gram per fish; fourthly, 1 / 3 of water is changed each month from January to June, 1 / 3 of water is changed each half month from July to September, and 1 / 3 of water is changed each month from October to December; fifthly, food organism is supplemented timely when the density of the food organism is lower than 1 per litre; sixthly, during a cultivation period, artificial pellet feed is supplemented as the feed for the scatophagus argus, opossum shrimp and crustacean 1 to 2 jin per mu and 1 time per day; finally, fishes are captured with nets in early winter, wherein the specification of captured coilia ectenes is 16 to 20 gram per fish while the specification of the scatophagus argus is 110 to 130 gram per fish.

The invention discloses a natural propagation method for coilia ectenes. According to the natural propagation method, parent fishes are bred according to the river and sea migration rule of the coilia ectenes, live baits adaptive to seawater or fresh water living are additionally thrown, pellet feed with vitamin and microelement added is additionally thrown, water flows of a parent fish pool are increased, salinity is regulated and controlled, a breeding fish bed is arranged, fertilization time of fertilized ova is controlled, the fertilized ova is hatched, fish larvas are bred, and fish fries are bred. The bottleneck problems of large-scale breeding of the coilia ectenes and industrialization propagation of the fries are solved, the final spawning rate is up to 98%, the fry obtaining rate is up to 90%, and the fries are strong and healthy.

The invention discloses a method for selecting specific SRAP (Sequence-Related Amplified Polymorphism) marking tapes for coilia ectenes genders and a gender determination method. The specific SRAP marking tapes for coilia ectenes genders are selected through SRAP-PCR reaction of 49 primer sets; and then the DNA sample of the coilia ectene which is not clear in gender is amplified through a specific primer set; and the gender of the coilia ectenes is differentiated according to whether the corresponding specific marking tapes can be amplified or not. The method provided by the invention overcomes the limits and shortcomings of the existing coilia ectenes gender differentiation technology, and therefore, an accurate and convenient gender differentiation method is provided.

The invention discloses a mixed feed for lobsters. The mixed feed comprises, by weight, 20-35 parts of fish meal, 15-20 parts of Coilia ectenes Jordan, 5-8 parts of meat and bone meal, 15-20 parts of flour, 8-10 parts of corn, 11-15 parts of soybean meal, 4-8 parts of rapeseed meal, 2-4 parts of fish oil, 10-15 parts of corn protein powder, 1-3 parts of pumpkin seed powder, 4-6 parts of sweet rice flour, 0.1-0.3 parts of choline, 2-3 parts of edible salt, 3-6 parts of grease, 2-5 parts of bentonite, 0.1-0.3 parts of an immunopotentiator, 0.1-0.3 parts of a liver protection agent, 0.1-0.2 parts of multiple vitamins, 0.1-0.2 parts of multiple mineral matters and 1-2 parts of calcium dihydrogen phosphate. Compared with feeds in the prior art, the mixed feed for lobsters, provided by the invention, has the advantages of abundant sources of raw materials, enhancement of the disease resistance and the immunity of lobsters due to inclusion of drug components, high weight gain rate of the lobsters, small dosage, and high survival rate of the lobsters.

The invention discloses a method for large-scale ecological aquaculture of coilia ectenes in a greenhouse. The method which adopts semi-running-water ecological aquaculture in the greenhouse includes: 1) water source selection; 2) aquaculture facility configuration; 3) ecological aquaculture; 4) adult fish harvest. Underground water of a deep well deeper than 30m is selected to serve as the water source, and an eel formula feed is used as a powdery formula feed. The method for large-scale ecological aquaculture is large in aquaculture scale, high in aquaculture density and high in yield, and coilia ectenes bred according to the method is high in survival rate and extremely high in nutritive value.

The invention aims at providing a method and use feed for artificial reproduction of lateolabrax maculatus. The use feed for the artificial reproduction of the lateolabrax maculatus is prepared from main materials and additives, wherein the main materials comprise lateolabras maculatus matched feed, trash fishes of mullets, coilias, mackerels and the like, fresh shrimps, squids and oysters; the additives comprise vitamin B12, vitamin C, vitamin E and gonad maturation promoting treasure. The preproduction method comprises the following steps: firstly, screening parent fishes and improving nutrient; secondly, ripening and carrying out artificial propagation; thirdly, collecting and incubating fertilized eggs. The method has the advantages that physique of the parent fishes can be significantly enhanced, the development of gonad is promoted, the quality of sperms and eggs is optimized and the quality of gametes is improved; meanwhile, the repeated utilization rate of the parent fishes isimproved; by using proper drug dose of ripening and artificial propagation, the parent fishes can concentrate on spawning, so that the labor intensity and reproduction cost of artificial reproductionare reduced; the method for the artificial reproduction of the lateolabrax maculatus has high success rate, so that the yield of lateolabrax maculatus fries can be effectively improved and the demandsof large-scale culturing are met.

The invention discloses a molecular marker for discriminating different ecotype populations of coilia ectenes. A polymorphic site of coilia ectenes retrotransponsons SINE is selected as a target, and according to the flanking sequence of insertion sites, a specific primer is designed, DNA fragments with SINE inserted or with lost sites are obtained through PCR (Polymerase Chain Reaction) amplification, agarose gel electrophoresis, molecular clone and the like, and the specific DNA fragments can be used for discriminating different populations of coilia ectenes. According to the invention, the populations of coilia ectenes can be rapidly and accurately determined, and the molecular marker can be widely used in the aspects of assistant breeding, artificial releasing effect evaluation of fry, and dynamic monitoring of populations.

The invention discloses artificial propagation and domestication methods of coilia ectenes, relating to a propagation and domestication method of fishes. The artificial propagation method comprises the steps of parent fishing, artificial insemination and incubation in a laboratory; and the domestication method comprises the steps of: 1. putting coilia ectenes fries of uniform size in a glass jar with water, wherein the main feeding bait is composed of 10 kinds of bait such as a compound feed, rotifers and the like, the average water temperature in the feeding period is 25 DEG C; the content of dissolved oxygen is higher than 6 mg / L, the content of ammonia nitrogen is lower than 0.14 mg / L, and the bait composite is fed four times every day; and 2. using the fish at the first stage as materials and continuing to feed the fish for 60 days, wherein the density of released is 50 fish per square meter, the same artificial feed is used in the period, and the feeding condition and temperatureare same. Therefore, mass propagation of coilia ectenes is ensured, the living rate is high, the weight of artificially domesticated coilia ectenes is increased, and the requirement of economical production is met.

Provided is a micro-oxygenating long-distance transport method for indoor breeding of seedlings of coilia ectenes. The method is characterized in that from the mid-term of July to the mid-term of August each year, seedlings of coilia ectenes undergoing artificial breeding grow to 3-5 cm; a dragging net for fries cultured in an indoor cement pool for all kinds of breeding fields; one day before seedlings of coilia ectenes are dragged into the net and transported, seedlings take exercises by net dragging for 2-3 times; seedlings stop eating for 12 hours before transport; fries are counted in a water bucket with the volume of 10 liters; each bucket contains 50-100 fish fries which are then transferred to a transport water tank; the transport water tanks are inflated with air by oxygen bottles with pure oxygen nanotubes; after fish tries arrive at the destination, water temperature of the fish pond and water temperature of the water tanks are detected; after fish tries in the water tanks are stabilized for 10-15 minutes, a spoon with the 1L volume is utilized for scooping up fish fries to the water tanks with the volumes of 10 L; and each water tank can hold 30-50 fish fries and fish fries are taken to the fish pond with water.

The invention belongs to the technical field of aquaculture and provides a coilia ectenes offspring pond breeding enemy prevention method. Before 1-2days of artificial insemination on a coilia ectenes, thorough disinfection and pond cleaning are carried out by adopting disinfecting drugs formed by mixing of quick lime and bleaching powder; water quality regulation and control and filtering treatment are carried out on a water source which is injected into a coilia ectenes nursery pond; enemy creature prevention devices are arranged on the periphery and above the coilia ectenes nursery pond; and each enemy creature prevention device is composed of a steel pipe pile body, a main wire, a steel wire rope and a rubber thread net. By means of the thorough disinfection and pond cleaning through the drugs, serious filtering on entering water and arrangement and optimization of the enemy creature prevention devices, troubles from enemy creatures are reduced to the maximum, seedling survival rates are improved, reliable guarantee is supplied to large-scale artificial breeding and industrialization development of the coilia ectenes, practicability is strong, and popularization and application value is strong.

The invention provides a primer for identifying coilia ectenes and coilia brachygnathus and a molecular biological method. The primer contains an amplified primer group and an extended primer group. A nucleotide sequence of the amplified primer group is shown as SEQ ID NO:1-16, a nucleotide sequence of the extended primer group is shown as SEQ ID NO:17-24. The primer combination can be effectively used for identifying the coilia ectenes and the coilia brachygnathus. The molecular biological method comprises the steps that a to-be-tested coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequencing library is established, sequencing is conducted on the library to obtain a sequencing result; based on the sequencing result, a coilia ectenes and coilia brachygnathus SNP locus area nucleic acid sequence is determined; then Structure2.3.2 software is utilized to draw a column diagram of population genetic differences and a column diagram of individual genetic differences, and species are judged by utilizing analysis. The method includes simple and convenient steps and is accurate, efficient and low in cost.

The invention relates to a method for drawing blood from coilia ectenes under anaesthesia, and the operation steps are as follows: firstly adding mixed water of pond water and deep well water into a container, regulating the water temperature to 10-20 DEG C, and adding edible salt and tricaine sulfonate into the mixed water for preparing anaesthetic solution, wherein the salt degree is 5-7 per mill and the percentage concentration of the tricaine sulfonate is 0.01%-0.03%. The coilia ectenes is placed in the anaesthetic solution, and the anaesthetic time is 2-4 minutes. A syringe is used for drawing the blood, the part is located at the bottom of the spine above the anus, the time for drawing the blood is 1-1.5 minutes, and the coilia ectenes after drawing the blood is transferred into a temporary cultivation pond for regaining consciousness. The method can successfully carry out anaesthesia and blood drawing on the coilia ectenes against the physiological characteristics of the coilia ectenes, the method is simple, the cost is low and the volume of drawing the blood is large.

The invention discloses a molecular marker for identifying different ecotypic species of coilia ectenes. Polymorphic loci of retrotransponsons SINE of coilia ectenes are selected as targets, and a specific primer is designed according to flanking sequences inserted into the loci; DNA segments of the SINE insertion or deletion loci are obtained by applying the methods such as PCR amplification, agarose gel electrophoresis, molecular cloning and the like, and different species of the coilia ectenes are identified by using the specific DNA segments. By adopting the molecular marker, the species of the coilia ectenes can be quickly and accurately determined, and the molecular marker has extensive use in the aspects such as molecular marker-assisted breeding of the coilia ectenes, artificial discharge effect assessment of fries, dynamic species monitoring and the like.

The invention discloses a soil pond half-flowing-water culture method for Yangtze coilia ectenes. The method comprises the following steps: 1, adjusting the environmental conditions of a pond; 2, culturing fish fries; 3, managing the fish fries daily; 4, culturing adult fish; 5, managing the adult fish daily; 6, preventing and curing diseases; and 7, harvesting. With the adoption of the provided culture method for the Yangtze coilia ectenes, the growth cycle is relatively long, the weight of the adult fish is about 100-150g, the adult fish has a well-developed body and tastes very delicious, and the cultured Yangtze coilia ectenes is very much like wide Yangtze coilia ectenes.

The invention provides a juvenile coilia ectenes feed additive and a preparation method. The additive is prepared from the following raw materials: chitosan, digestive enzyme, carriers, lysine, glycine, glycine betaine, tea polyphenol, organic selenium, vitamin C, fish oil, emulsifying agent, sodium alginate, gelatin and citric acid. The preparation method comprises the following steps: preparing the digestive enzyme into a digestive enzyme solution, adsorbing with the carriers, then adding the sodium alginate, the gelatin and the citric acid into water, heating, preserving the heat, adding the emulsifying agent while stirring to obtain a mixed solution, adding the digestive enzyme carrier solution, the chitosan, the lysine, the glycine, the glycine betaine, the tea polyphenol, the organic selenium, the vitamin C and the fish oil into the mixed solution, homogenizing at the high pressure, spray drying, and preparing the feed additive. The additive provided by the invention provides sufficient nutrition to the juvenile coilia ectenes, can increase the survival rate of the raised juvenile coilia ectenes, capable of enabling the juvenile coilia ectenes to have the raising value, capable of improving the fertilizer efficiency and capable of decreasing the feed coefficient.

The invention discloses an asplanchna eliminating method for early-stage indoor Yangtze river coilia ectenes seedling culture. The method is characterized in that during early-stage culture of coilia ectenes seedlings which are 12-18 days old, when asplanchna multiple rapidly and grow, salt is increased step by step until the salinity reaches 5, after the salinity reaches 5 through salt increasing, stabilization is conducted for 48 hours, during salinity stabilization, brackish water with the salinity of 5 is adopted for water changing, and asplanchna which sink to the bottom are eliminated in time; during asplanchna elimination through salt increasing, feeding with brachionus angularis and brachionus calyciflorus is stopped, and switching is conducted with brachionus urceus which are salvaged and collected with a bolting-silk net of 150 meshes and filtered with a bolting-silk net of 80 meshes; after 95% of the asplanchna sink to the bottom, salt is decreased step by step, pure water is adopted as salt decreasing water, and in the salt decreasing process, bottom suction is conducted once in the morning and afternoon respectively everyday to further eliminate the sunken asplanchna thoroughly; two days after step-by-step salt decreasing, the salinity of water in a seedling culture pond drops to 2 or below, and all original operations of seedling culture are restored.