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Method for drawing blood from coilia ectenes under anaesthesia

A technology of anchovy and syringe, applied in the field of anesthesia and blood drawing of anchovy, can solve the problems of low efficiency, slow work progress, and easy death, and achieve the effect of low cost, large amount of blood drawn, and easy-to-learn method

Inactive Publication Date: 2010-05-19
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficiency of extracting DNA and RNA from anchovy muscle and various organs and tissues currently used is low, and many work that needs to be measured by blood are progressing slowly
Anchovy is extremely delicate and easy to die. The existing simple treatment methods of only clove oil, tricaine sulfonate (MS-222) and blood drawing from the tail peduncle of common fish are no longer suitable for anesthesia and blood extraction of anchovy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] 1. Anesthesia

[0016] First add 1:1 mixed water of pond water and deep well water into the container, adjust the water temperature to: 10℃; add ordinary Huai salt (produced by Jiangsu Salt Industry Group Co., Ltd.) into the mixed water and add MS-222 to make it An anesthesia solution with a salinity of 5‰ and a MS-222 concentration of 0.01%; put a 50g body weight of Coilia serrata into the anesthesia solution, the anesthesia time is: 2 minutes.

[0017] 2. Blood draw

[0018] After the scalpel is anesthetized and turned its belly, move it into an iron plate with a diameter of 60 cm. The tail of the scalpel is slightly lifted to draw blood, and a 1ml disposable sterile syringe (produced by Changzhou Yuekang Medical Equipment Co., Ltd.) is placed above the anus of the scalpel. The blood is drawn from the bottom of the spine, and the time is 1.5 minutes; the blood volume is 0.3ml.

[0019] 3. Resuscitation

[0020] After the blood is drawn, the fish is transferred to an area of ​...

Embodiment 2

[0022] 1. Anesthesia

[0023] First add a 1:2 mixture of pond water and deep well water to the container, adjust the water temperature to: 15℃; add ordinary Huai salt (produced by Jiangsu Salt Industry Group Co., Ltd.) into the mixed water and add MS-222 to make it An anesthesia solution with a salinity of 6‰ and a percent concentration of MS-222 of 0.02%; put a 100 g body weight of Coilia occidentalis into the anesthesia solution, and the anesthesia time is: 2 minutes.

[0024] 2. Blood draw

[0025] After the scalpel is anesthetized and turned over, move it into a plastic tray with a diameter of 70 cm. The tail of the scalpel is slightly lifted to draw blood, and a 1ml disposable sterile syringe (produced by Changzhou Yuekang Medical Equipment Co., Ltd.) is placed above the anus of the scalpel. The blood is drawn from the bottom of the spine. The blood drawn time is 1.2 minutes; the blood volume is 0.6ml,

[0026] 3. Resuscitation

[0027] After the blood is drawn, the fish is trans...

Embodiment 3

[0028] Embodiment 3: A method for blood drawing with coilia anesthesia using the following process steps:

[0029] 1. Anesthesia

[0030] First add a 1:3 mixture of pond water and deep well water to the container, adjust the water temperature to: 20℃; add ordinary Huai salt (produced by Jiangsu Salt Industry Group Co., Ltd.) into the mixed water and add MS-222 to make it An anesthesia solution with a salinity of 7‰ and a percentage of MS-222 of 0.03%; put a 150 g body weight of Coilia spp. into the anesthesia solution for 2 minutes.

[0031] 2. Blood draw

[0032] After anaesthetizing the scalpel, move it into an enamel dish with a diameter of 65 cm, lift the tail of the scalpel slightly to draw blood, and use a 1ml disposable sterile syringe (produced by Changzhou Yuekang Medical Equipment Co., Ltd.) on the anus of the scalpel. The blood is drawn from the bottom of the spine, and the time is 1 minute; the blood volume is 1.0ml.

[0033] 3. Resuscitation

[0034] After the blood is dra...

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Abstract

The invention relates to a method for drawing blood from coilia ectenes under anaesthesia, and the operation steps are as follows: firstly adding mixed water of pond water and deep well water into a container, regulating the water temperature to 10-20 DEG C, and adding edible salt and tricaine sulfonate into the mixed water for preparing anaesthetic solution, wherein the salt degree is 5-7 per mill and the percentage concentration of the tricaine sulfonate is 0.01%-0.03%. The coilia ectenes is placed in the anaesthetic solution, and the anaesthetic time is 2-4 minutes. A syringe is used for drawing the blood, the part is located at the bottom of the spine above the anus, the time for drawing the blood is 1-1.5 minutes, and the coilia ectenes after drawing the blood is transferred into a temporary cultivation pond for regaining consciousness. The method can successfully carry out anaesthesia and blood drawing on the coilia ectenes against the physiological characteristics of the coilia ectenes, the method is simple, the cost is low and the volume of drawing the blood is large.

Description

Technical field [0001] The invention belongs to the technical field of fish breeding. Specifically, it relates to a method for drawing blood under anesthesia with coilia. Background technique [0002] Coilia nasus (Coilia nasus) is one of the valuable migratory fishes that enjoys the reputation of "Three Fresh Products of the Yangtze River". Research on the germplasm preservation and artificial propagation technology of Coilia nasus has always been the focus and hotspot of domestic and foreign experts. [0003] At present, with the rapid development of modern molecular biology technology and biochemical technology, various high-tech technologies and instruments such as DNA and RNA have also been continuously introduced into various research fields of Coilia. However, the efficiency of extracting DNA and RNA from the muscles and organs of Coilia spp. currently used is low, and many tasks that require blood to be measured are progressing slowly. Coilia vulgaris is extremely delicat...

Claims

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Application Information

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IPC IPC(8): A01K61/00C12Q1/68G01N1/14
CPCY02A40/81
Inventor 徐钢春顾若波徐跑郑金良张呈祥
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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