47 results about "Exopalaemon carinicauda" patented technology

The invention relates to a method for establishing an exopalaemon carinicauda family by applying in-vitro egg culture. The method comprises the steps that parent exopalaemon carinicauda are selected and disinfected, and the parent exopalaemon carinicauda and eggs are separated; shaking culture parameters of the eggs are measured; microscopic observation is performed in the culture process; the progressive decreasing control of salinity is performed in the culture process; young exopalaemon carinicauda after incubation are cultured. According to the method, the probability of incubation failure caused by influence of different factors in the egg laying process of the parent exopalaemon carinicauda can be effectively lowered, the incubation rate of the eggs and the survival rate of the young exopalaemon carinicauda are increased, and therefore the method has good application prospects in the application of establishing the exopalaemon carinicauda family.

The invention discloses a method for synchronous line establishment of small-population parents in artificial breeding of exopalaemon carinicauda. In non-high-breeding season at relatively low water temperature, water temperature is regulated and controlled in three development stages (parent prawn gonad development stage, zygote embryo development stage and larval development stage) by virtue of a temperature control method, so that families established by small-population parents in different gonad development stages can develop into a post-larval stage at the same time as much as possible, and the synchronous line establishment of the small-population parents is finally achieved. By respectively setting temperature gradients according to the suitable temperature ranges of the exopalaemon carinicauda in different development stages, the method disclosed by the invention is conducive to the shortening of development time difference of various parent progenies, and capable of achieving the synchronous line establishment and multi-generation breeding per year of the small-population parents in the artificial breeding of the exopalaemon carinicauda.

The invention provides a method for ecologically and early breeding Yangtze River coilia ectenes in a pond greenhouse, and relates to coilia ectenes cultivation. The method comprises production steps of parent overwintering and strengthening cultivation, breeding parent stocking, mysis inoculation, daily management, fish fry number estimation and parent removing. The method is characterized in that when the water temperature of an outer pond is reduced to 7-8 DEG C from December to January in the following year, healthy two-age coilia ectenes without surface damage is taken as parent fish and transferred to the pond greenhouse for overwintering and strengthening cultivation; after the parent fish enters the greenhouse, 3 kilograms of exopalaemon carinicauda are put into the pound for each mu, and the mysis is inoculated; from the end of March to the middle ten days of April, the water temperature rises to 15-16 DEG C, overwintered and strengthened parent fish is picked and transferred to an ecological breeding pond, growth and feeding conditions of the fish fry are checked every one week, and the density of fish bait in water is adjusted according to the checked conditions; a seine is used for removing the parent fish in the middle ten days and last ten days of June, 1, 000-2, 000 fish fry can be generally seined each mu, the fish fry specification is that the total length is 7-10 cm and the weight is 1.8-2.5 g, and the fish fry can be taken as culturing offspring seeds.

The invention provides a method for constructing an Exopalaemon carinicauda reciprocal backcross family. The method includes the following steps: (1) parent shrimp selection; (2) parent shrimp mating; (3) cultivation and breeding of F1 offspring seeds; (4) parent-child backcross; and (5) cultivation and breeding of backcross progeny offspring seeds. Male and female Exopalaemon carinicauda parent shrimps which are distributed naturally or bred artificially and have excellent characters are selected, and the reciprocal backcross family can be acquired through completely-artificial breeding, aquaculture and parent-child mating; the success rate of construction of the reciprocal backcross family can reach 75%, and can provide material and technique support for quantitative character genetic analysis, near-isogenic line construction and back cross breeding of the Exopalaemon carinicauda.