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Method for breeding saline-alkaline-tolerant strain of exopalaemon carinicauda

A technology of ridgetail white shrimp and salt-alkali tolerance, applied in fish farming, application, climate change adaptation, etc., to achieve good market application prospects, strong stress resistance, and suitable for promotion

Pending Publication Date: 2021-01-29
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation of new varieties of white prawns started in 2008, and the breeding was mainly carried out for growth and body color traits. A new fast-growing variety of white prawns "Huangyu No. 1" and a new variety of white prawns with red body color "" have been bred. Kesuhong 1", but there is no report on the breeding method of the salt-alkali tolerance strain of the white shrimp

Method used

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  • Method for breeding saline-alkaline-tolerant strain of exopalaemon carinicauda

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1: The parental source and selection process of the white shrimp "Huang Yu No. 1"

[0024] 1. Parent source

[0025] In 2011, the parents came from 5 wild geographical groups along the coast of my country, including about 5,600 in the Bohai Bay area, about 5,400 in the Laizhou Bay area, about 5,200 in the Jiaozhou Bay area, about 5,800 in the Haizhou Bay area, and about 8,000 in the Xiangshan Bay area. , a total of about 30,000 tails.

[0026] 2. Cultivation process

[0027] A new fast-growing variety of white prawn was selected by group selection method.

[0028] From May to November 2011, wild broodstock in five sea areas of Bohai Bay, Laizhou Bay, Jiaozhou Bay, Haizhou Bay and Xiangshan Bay were collected as basic groups, and genetic diversity analysis was carried out; individuals were selected from each basic group 5,000 large and healthy broodstock without damage were used to construct a core breeding group.

[0029] In 2012, group selection was used to ...

Embodiment 2

[0034] 1. Determination of the semi-lethal alkalinity of 30-day-old white shrimp

[0035] In this embodiment, four carbonate alkalinity gradients of 5mmol / L, 10mmol / L, 15mmol / L, and 20mmol / L were set up for semi-lethal experiments, and the number of dead white shrimps in each experimental gradient group was counted at 12h, 24h, 48h, and 72h , the calculated semi-lethal alkalinity of 30-day-old white prawns at 72 hours was 8.26mmol / L.

[0036] Table 1 Determination of the semi-lethal alkalinity of the white shrimp

[0037] coercion time 12h 24h 48h 72h Half lethal concentration mmol / L 11.83 10.31 9.84 8.26

[0038] 2. Estimation of the heritability of salinity-alkaline tolerance traits in the white shrimp

[0039] The heritability of salt-alkaline tolerance traits in 50-day-old white shrimp was estimated by whole-sib group intracorrelation method. 50 full-sib families (including 42 half-sib families) were constructed by directional mating, and the s...

Embodiment 3

[0044] The breeding method of the saline-alkali-resistant strain of the white shrimp of the present invention comprises the following steps:

[0045] (1) Select the cultivated "Huangyu No. 1" white prawn as the basic population.

[0046](2) At the end of May 2017, according to the requirements of 5% seed retention rate and 1:1 ratio of male to female, healthy juveniles that had grown to 30 days old were selected from the basic population of "Huang Yu 1" with excellent growth characteristics. About 20,000 shrimp were subjected to carbonate alkalinity stress. The salinity stress was carried out in the indoor cement pond, the stress alkalinity was controlled at 8.26mmol / L, and the stress was continued for 72 hours. About 10,000 surviving individuals were screened out to establish a core population for salt-alkali tolerance breeding, and the culture continued in the breeding pond.

[0047] (3) The core group of salt-alkali-tolerant breeding adopts a mating design with a male-to-f...

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Abstract

The invention provides a method for breeding a saline-alkaline-tolerant strain of exopalaemon carinicauda. The method comprises the following steps of selecting artificially bred multi-year exopalaemon carinicauda with excellent growth traits as a basic group; performing an alkalinity stress experiment on young exopalaemon carinicauda growing to 30 days old; performing continuous three-generationalkalinity stress breeding to screen out a saline-alkaline-tolerant strain of the exopalaemon carinicauda; and building a core breeding group capable of being used for culture application after passage propagation. The method provided by the invention has the advantages that a rapid growth group is selected as a basic group; through saline-alkaline-tolerant breeding and polymerization of saline-alkaline-tolerant stress resistance properties, the bred new strain grows rapidly, has high stress resistance, and is suitable for the breeding in saline-alkaline regions; the growth speed is obviouslyaccelerated; the breeding survival rate is obviously improved; the operation of the method provided by the invention is simple and convenient; and the method provided by the invention is suitable forthe breeding of the saline-alkaline-tolerant strain of the exopalaemon carinicauda.

Description

technical field [0001] The invention belongs to the field of aquatic genetics and breeding, and in particular relates to a method for selecting a salt-alkali-resistant strain of white prawn. Background technique [0002] Exopalaemon carinicauda, ​​also known as white shrimp, small white shrimp and winter jasmine, belongs to Arthropoda, Crustacea, Decapoda, Palaemonidae, white shrimp The genus (Exopalaemon) is mainly distributed in the shallow sea and low-salt waters along the coast of the entire mainland of China and the west coast of the Korean Peninsula. The Bohai Sea and the Yellow Sea have the highest production. It is one of the three unique economic shrimps in China. chinensis) and Chinese hairy shrimp (Acetes chinensis). In recent years, the aquaculture area of ​​white prawns has expanded rapidly. According to incomplete statistics, the current aquaculture area of ​​white prawns in the country has reached 400,000 mu, with an output of more than 50,000 tons. It has be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 李吉涛李健刘萍陈萍王佳佳
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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