The invention discloses a seedling culture method for promoting pinus massoniana asexual nursery stock growth. The seedling culture method includes the steps of seedling culture cup selecting, matrix preparing, cup filling, disinfecting, seedling transplanting and water and fertilizer managing; peat soil, pinewood epidermises, yellow soil, pumice stone, fermented pine bark and coco coir are mixed by a ratio to obtain a matrix, an appropriate amount of controlled release fertilizer is added and stirred fully, the yellow soil and the matrix are loaded into non-woven bag seedling culture cups in the mode of putting the yellow soil in center holes and putting light matrix outside, the matrix is disinfected before seedling transplanting, then pinus massoniana asexual seedlings are transplanted into the seedling culture cups, water and fertilizer managing are carried out at regular time after transplanting until nursery stock is output for sell. The method has the advantages of being easy to operate, sufficient in resource, low in cost, high in fertilizer efficiency, good in absorption and beneficial for growth of pinus massoniana asexual nursery stock, the cultured nursery stock is high in quality, strong and high in growth speed, and the method provides technical support for pinus massoniana development and has good economic benefits, social benefits and ecological benefits.