Artificial pollination breeding method of pinus massoniana hybrid
A technology of artificial pollination and masson pine, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, can solve the problems of poor stem shape straightness, great influence of natural conditions, slow growth in the early stage, etc. Straight shape, low cost and rapid growth in the early stage
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Embodiment 1
[0026] In March 2010, the improved generation seed garden of Pinus massoniana in the state-owned Paiyangshan Forest Farm in Guangxi Zhuang Autonomous Region was artificially controlled with Pinus massoniana as the female parent and Pine slash, Pine scum, Pinus caribbean and Pine-resistant pine caterpillar as the male parent. In the pollination cross experiment, a total of 141 combinations were pollinated. From October to November 2011, 131 combinations of hybrid masson pine seeds were harvested, and the remaining 10 combinations failed to pick cones due to branches broken by wind or man-made damage, with a total of 948.26 grams. Conventional methods were used to raise seedlings, and now Afforestation carried out progeny determination test, and the growth of seedlings was good.
Embodiment 2
[0028] In March, 2011, the supplementary test of embodiment 1 was carried out in the improved seed garden of Pinus massoniana in the state-owned Paiyangshan Forest Farm of Guangxi Zhuang Autonomous Region, and 90 combinations were pollinated in total. From October to November 2012, 90 combinations of hybrid masson pine seeds were harvested, totaling 155.44 grams. Conventional methods were used to raise seedlings. Now afforestation has been carried out for progeny testing, and the seedlings are growing well.
Embodiment 3
[0030] In March 2010, 47 Pinus massoniana were selected in the state-owned Paiyangshan Forest Farm of Guangxi Zhuang Autonomous Region to conduct artificial pollination hybridization experiments.
[0031] 1. Preparation before pollination
[0032] The masson pine with good growth and no pests and diseases was selected as the female parent of the plant, and the excellent individual slash pine with good growth and no pests and diseases in the stand was selected as the male plant, and the pollen of slash pine was collected in February. When collecting pollen, use branch scissors to cut 20cm-30cm well-grown branches of male flowers in the middle and upper part of the crown of the selected slash pine tree and bring them back to hydroponic culture. Collect pollen when the male flowers mature and loosen pollen. Put the collected pollen number of the male parent into a high-temperature sterilized brown glass bottle. At the same time, put an appropriate amount of desiccant in the bottl...
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