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Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana

A technology for regenerating plants and masson pine, which is applied in the fields of botanical equipment and methods, plant regeneration, and horticultural methods, and can solve the problems of low plant regeneration rate, difficulty in culturing organs, and difficulty in meeting production and application needs.

Active Publication Date: 2010-10-06
NANJING FORESTRY UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to solve the current problems such as the difficulties in tissue culture of Pine massoniana, the low plant regeneration rate, and the extremely low success rate of inducing somatic embryos with mature zygotic embryos of Pine massoniana, which is difficult to meet the needs of production and application. The ESM is a new way to induce the occurrence of somatic embryos and plant regeneration, quickly produce a large number of high-quality seedlings, and meet the needs of forestry production for high-quality masson pine seedlings

Method used

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  • Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana
  • Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana
  • Method for inducing embryonal-suspensor mass (ESM) regeneration plant from immature seed of pinus massoniana

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Embodiment Construction

[0163] The test materials were immature embryos of excellent trees of Pine masson, collected from the seed orchards of Pine masson clones grafted in Nanning, Guangxi and Nanping, Fujian, respectively, and repeated for 3 years. The cones are collected every 10 days from the end of May to the end of September every year. The seed development stages corresponding to each seed collection time point were the developmental stages from immature embryo to mature embryo. After the cones were collected, they were placed in a pre-frozen ice box on site and wrapped with moist gauze to prevent water loss; then they were quickly transported back to the laboratory and stored in a 4°C refrigerator for later use. Pretreatment at 4°C refrigerator for at least one week is conducive to the efficient induction of ESM. Before inoculation, take the spare cones out of the refrigerator, soak and clean them with detergent-containing lotion, and then rinse them with running water for 2 hours. Take out...

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Abstract

The invention relates to a method for inducing an embryonal-suspensor mass (ESM) regeneration plant from an immature seed of pinus massoniana. By using the characteristic of continuous schizogenesis capability of the ESM in the immature seed of the pinus massoniana, solid culture and liquid culture are performed alternatively and ESM forms a completely-grown embryo with a normal structure after a maintaining stage, a multiplication stage, an embryo pre-mature stage and a maturation culture stage, so that a pinus massoniana plant is obtained by germinating and culturing the embryo. Due to the adoption of the method, a large number of regeneration plants of a pinus massoniana embryo of which the hereditary basis is accordant can be obtained and new technology with a short period and high occurring efficiency is provided for the storage of pinus massoniana rare generic resource and the mass production of excellent-gene nursery-grown plants.

Description

1. Technical field [0001] The invention belongs to the technical field of seedling propagation in forestry, and in particular relates to a method for inducing plant regeneration from immature seeds of immature seeds of Pinus massoniana (Embryonal Suspensor Mass, ESM for short). 2. Background technology [0002] Masson pine (Pinus massoniana) is one of the main timber tree species in southern my country, with a wide distribution and a large stock volume. Because of its strong adaptability, fast growth, wide range of uses, easy reforestation and low cost, it has played a great role in greening barren hills and improving the ecological environment. Compared with commercial Chinese fir and northern red pine, Masson pine surpasses Chinese fir and red pine in terms of bending strength, flexural modulus, pressure along the grain, and end surface hardness; and its wood cellulose content is higher, and its wood is widely used Used in construction, panel, furniture, plywood, paper an...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 施季森高燕席梦利边黎明陈金慧郑仁华黄寿先
Owner NANJING FORESTRY UNIV
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