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Method for preparation of pinus massoniana lamb superior individual sterile explant and induction of initial bud

An explant and masson pine technology, applied in the field of plant reproduction, can solve the problems of difficulty in disinfection of explants, low initial bud occurrence rate, poor sprout activity, etc., and achieves high initial bud occurrence rate, good economic benefits and social benefits. Benefit, the effect of rapid sprout elongation

Active Publication Date: 2015-07-08
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical problems such as difficult explant disinfection, browning, low incidence of initial buds, poor activity of sprouts, etc. in the tissue culture of the existing excellent adult Pinus massoniana, and provides a preparation of excellent single-plant aseptic masson pine explants and the method of initial bud induction

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old masson pine tissue culture seedlings as the rootstock, and selecting the adult masson pine excellent plant GL38 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm Take tender shoots as scions. The scion was cut from an adult masson pine mother tree, wrapped in a wet towel, put into an incubator with an ice pack, brought back to refrigerated at 4°C, and grafted within 24 h by conventional methods. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouts reached a length of 3.0-5.0 cm, they were sprayed with a volume concentration of 0.1% carbendazim in a conventional way. After 1-2 days, they were harvested in fine weather and vigorously harvested. Long sprout strips with a length of 3.0-5.0 cm were used as explan...

Embodiment 2

[0027] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old masson pine tissue culture seedlings as the rootstock, and selecting the adult masson pine excellent plant GL38 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm Take tender shoots as scions. The scion was cut from an adult masson pine mother tree, wrapped in a wet towel, put into an incubator with an ice pack, brought back to refrigerated at 4°C, and grafted within 24 h by conventional methods. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouting strips reached 3.0-5.0 cm long, they were sprayed with a volume concentration of 0.1% carbendazim solution by conventional methods. , the long sprout strips with a length of 3.0-5.0 cm are used as explants for future use.

[0028] After the explants were rinsed ...

Embodiment 3

[0032] A method for the preparation of aseptic explants of an excellent single plant of Masson pine and the initial bud induction method, using half-year-old tissue cultured seedlings of Masson pine as rootstock, and selecting the adult masson pine excellent plant GL105 from the excellent forest stand of Guangxi State-owned Paiyang Mountain Forest Farm. Take tender shoots as scions. The scion was cut from an adult masson pine mother tree, wrapped in a wet towel, put into an incubator with an ice pack, brought back to refrigerated at 4°C, and grafted within 24 h by conventional methods. After the grafting survived, the main shoots of the grafted seedlings were knocked out to promote the growth of buds. When the sprouts reached a length of 3.0-5.0 cm, the conventional method was used to spray a 0.1% thiophanate-methyl solution with a volume concentration. Vigorous, long sprouting strips measuring 3.0-5.0 cm were used as explants for future use.

[0033] After the explants were ...

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Abstract

The invention discloses a method for preparation of a pinus massoniana lamb superior individual sterile explant and induction of an initial bud. The method comprises the following steps: grafting by selecting a sprout twig with a newly grown tender top in the current year of an adult superior individual as a scion and taking a pinus massoniana lamb tissue culture seedling as a stock, performing disinfection treatment on the sprout twig by adopting a series of disinfectants to match for disinfection, and putting the explant scion in an optimized induction culture medium to perform initial bud induction to obtain the sterile explant and the pinus massoniana lamb initial bud with rapid growth and vigorous vitality. By adopting the method disclosed by the invention, the technical problems of difficulty in disinfection of the explant, browning, low incidence of the initial bud, poor activity of a bud shoot and the like in tissue culture of the pinus massoniana lamb adult superior individual are solved, the incidence of the initial bud and the activity of the bud shoot are improved, the robust explant which is sterile above 90% can be obtained, and better economic benefits and social benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to tissue culture and seedling raising technology, in particular to a method for preparing excellent single aseptic explants of Pinus massoniana and inducing initial buds. Background technique [0002] Masson pine ( Pinus massoniana Lamb.) belongs to Pinaceae (Pinaceae) Pinus subfamily (Pinoideae) Pinus ( Pinus L.) Dimensional vascular Pinus subgenus ( Subgen Pinus ) plant, which is a dual-purpose tree species unique to native afforestation in subtropical regions of my country. As a tree species with high comprehensive utilization value and broad promotion prospects, masson pine can not only be used to produce three-board, papermaking and chemical fiber industrial manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. In recent years, with the intensification of contradictions between people's growing living needs and the s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 姚瑞玲王胤苏烜雷军芳
Owner GUANGXI FORESTRY RES INST
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