The invention relates to a
microsatellite multi-PCR (
Polymerase Chain Reaction) for a
turbot paternity test, which comprises the steps of: extracting
DNA from an individual
turbot, screening pleomorphic
microsatellite markers of the
turbot, synthesizing a primer, constructing turbot multi-PCR systems and carrying out genealogy and
paternity tests. In the method, two multi-PCR systems are established for the turbot genealogy and
paternity tests, the steps of multi-PCR amplification,
electrophoresis and silver
staining are carried out on an individual to be tested so as to acquire
microsatellite marker gene type data,
cluster analysis for a genealogy test is carried out by adopting NTsys sofeware, and the paternity test is carried out by adopting Cervus2.0
software. The invention realizes that four microsatellite sites are simultaneously detected in one PCR, thus compared with the traditional
PCR method, the efficiency of the method is improved by around 4 times, and the method can be subjected to the field test in a breeding base. The invention has the characteristics of high efficiency, economy, simplicity, convenience, easy operation and the like and can be popularized and applied in the aspects of
germplasm identification, genealogy management and
selective breeding of fine breeds for the turbots.