67 results about "Insertion deletion" patented technology

The invention relates to a high-flux sequencing data analysis method and a device. The high-flux sequencing data analysis method comprises the steps of acquiring high-flux sequencing data of a sampleand a reference genome sequence, comparing the high-flux sequencing data with the reference genome sequence, and respectively acquiring locus data of single nucleotide variation (SNV) and locus data of insertion deletion mutation (Indel), and respectively filtering noise points of the SNV data and the Indel data through comparing the difference substantial degrees of the variation and the background, thereby obtaining the variation data. The invention further provides a device for analyzing the high-flux sequencing data and a computer-readable storage medium for storing instructions.

Provided herein are methods and kits for modulating the amplification efficiency of nucleic acids, which are useful in multiplex reactions where the amplification efficiency of one or more nucleic acids in the mixture are desired to be modulated relative to one or more other nucleic acids. Embodiments relate to molecular diagnostics, including detecting sequence variants, such as SNPs, insertions deletions, and altered methylation patterns, as well as the modulation of the amplification efficiency of internal control sequences to provide more accurate control sequences for amplification reactions.

The embodiment of the invention provides a multi-star emergent task planning method and device. The method comprises the steps that according to the task completion time limit of an emergent task, a ground station and a visible time window of each remote sensing satellite, the latest observation completion time of each emergent task on each remote sensing satellite, then, according to the latest observation completion time, the usable visible time window for observing the emergent task is screened out, finally, the emergent task is inserted into the usable visible time window where the emergent task can be inserted, and therefore the task completion time limit for the emergent task is applied to an emergent task plan so as to ensure that the emergent task can be completed before the task completion time limit; compared with an existing scheme, the method is more reasonable. In addition, when the emergent task cannot be directly inserted, conflict insertion deletion is designed according to the conflict priority substitution degree, one or more conflict tasks are deleted, the emergent task and conventional task insertion probability is increased, and the benefits of the whole schemeare increased.

The invention provides a method for identifying reality and purity of pepper male sterile three-line mating hybrids based on InDel (insertion-deletion) molecular markers. The method includes the steps: culturing the pepper hybrids and parents to a bud and seedling stage; extracting DNA (deoxyribonucleic acid); screening the hybrids and the parents by core primers. Primers of co-dominant markers can serve as molecular markers for identifying the reality of the hybrids, and co-dominant markers of the pepper hybrids and the parents continues to be screened by combining a secondary core primer library until the co-dominant markers are screened out if the co-dominant markers of the pepper hybrids and the parents cannot be screened out in a core primer library. According to the method, a systemcapable of rapidly and simultaneously identifying the reality and the purity of the pepper male sterile three-line mating hybrids is built and cannot be limited by time and seasons, indoor rapid identification of the reality and the purity of the hybrids is achieved, and molecular biology bases are provided for protection and market supervision of the pepper male sterile three-line mating hybrids.

The invention discloses TuVIPP1 protein and an encoding gene and application of the TuVIPP1 protein. The protein is the protein in (1) or (2). The protein in (1) is composed of amino acid residues shown in the sequence 2 in a sequence table; the protein in (2) has the same function, is derived from (1) and is obtained after an amino acid sequence residue sequence in the sequence 2 in the sequence table is subjected to replacing and / or deleting and / or adding of one or more amino acid residues. Experiment results show that the TuVIPP1 gene is cloned in diploid Urartu wheat, the key function of the gene in inner capsule generation is verified by transgenic complementary of arabidopsis thaliana Atvipp1T-DNA insertion deletion mutant, plant photosynthesis can be promoted, and albino plants are greened.

The invention discloses a hybrid capture kit for detecting tumor individually-medicated sixteen gene hot spots. The hybrid capture kit comprises a hybridization reagent, a PCR amplification reagent and an enrichment degree detection reagent, wherein the hybridization reagent comprises an SEQ NO.1 to SEQ NO.396 probe mixture, a molar ratio of every two probes is 1:1, and the use volume of the probemixture with the concentration being 10 nM each during detection is 1 [mu]l. The kit is gene detection based on a second-generation sequencing technology, can detect three variation types (mutation,insertion deletion and fusion) one time, and uses a high sequencing depth to cover micro-scale gene variation in order to accurately detect the mutation condition of a patient on the premise of limited samples, and the mutation frequency of 1% can be accurately detected. Polygene and high-sensitivity parallel detection improves the detection rate of medicated gene variation, so the medication opportunity is not missed; and hot spots, rare and even unknown gene variation cane be detected during one-time detection, and relevant drug-resistant mutations (such as KRAS and ALK point mutation) are detected when drug-sensitive variation is found, so the sensitivity and drug resistance information of the medicated patient is accurately reflected.

The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, and Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.

The invention discloses an insertion deletion marker site for identifying a shape of a watermelon fruit, a primer and application, and belongs to the technical field of biology. The insertion deletionsite is located in an 159bp area between nucleotide 26847023 and nucleotide 26847181 of a third chromosome of a watermelon genome 97103, and a sequence of the insertion deletion area is shown as SEQID NO. (sequence identifier number) 1. The invention further discloses a PCR amplification primer pair for identifying the shape of the watermelon fruit, a method of identifying the shape of the watermelon fruit, and application of the method. The insertion deletion marker site is developed on a basis of a candidate gene and tightly linked with the length and circularity of the fruit; and an insertion deletion marker can be used for accurately and quickly identifying the shape of the watermelon fruit in a seedling stage and has the advantages of convenient detection and stable amplification products.

The invention belongs to the technical field of creature identification, and particularly relates to an identification method for a hybrid rice variety hybrid source superior 69 based on an InDel (insertion-deletion length polymorphism) marker. In the method, the whole genome DNA sequence of a japonica rice variety Nipponbare and the whole genome DNA sequence of an indica type rice variety 93-11 are compared to obtain 14 pairs of specific DNA primers designed for an insertion / deletion differential fragment; the hybrid source superior 69 and parent hybrid sources 5A and JP69 of the hybrid source superior 69 are subjected to DNA extraction, DNA fragment amplification and electrophoretic separation, and the electrophoretogram is analyzed for identifying the hybrid source superior 69; to be specific, a finger-print spectrum obtained through polymerase chain reaction and gel vertical slab electrophoresis is analyzed by virtue of the combination of the 14 pairs of InDel primers, and further, whether the variety is the hybrid rice variety hybrid source superior 69 or not is determined according to the electrophoretic band type of 14 InDel sites. According to the invention, the sample amount required to be detected is small, the method is simple, convenient and quick, and the identification result is accurate, so that the method can be applied to the variety identification of the hybrid source superior 69 on the market.

T cells, notably CD8 T cells, are known to be essential players in tumor eradication as the presence of tumor-infiltrating lymphocytes (TILs) in several cancers positively correlates with a good prognosis. To eliminate tumor cells, CD8 T cells recognize tumor antigens, which are MHC I-associated peptides present at the surface of tumor cells, with no or very low expression on normal cells. Described herein a proteogenomic approach using RNA-sequencing data from cancer and normal-matched mTEChi samples in order to identify non-tolerogenic tumor-specific antigens derived from (i) coding and non-coding regions of the genome, (ii) non-synonymous single-base mutations or short insertion / deletions and more complex rearrangements as well as (iii) endogenous retroelements, which works regardless of the sample's mutational load or complexity.