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Molecule marker linked with brassica compestris color gene of brassica compestris and application of molecule marker

A technology of seaweed sprouts and seaweed sprouts, which can be applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. Synthetic and regulatory complexity issues

Active Publication Date: 2019-08-06
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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Problems solved by technology

In addition, there are also differences in the genetic mechanism of purple-regulated genes in different varieties. In different studies, it was found that some were qualitative traits controlled by a single gene, while others were quantitative traits with main effect loci.
These studies illustrate the high complexity of anthocyanin synthesis and regulation in Brassica

Method used

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  • Molecule marker linked with brassica compestris color gene of brassica compestris and application of molecule marker
  • Molecule marker linked with brassica compestris color gene of brassica compestris and application of molecule marker

Examples

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Embodiment 1

[0068] This example is the development of an InDel marker closely linked to the porphyra sap color gene.

[0069] Indel markers were derived from the resequencing data of Chinese cabbage and laver sprouts. That is to extract the genomic DNA of cabbage heart and seaweed sprout respectively, the concentration range is 800-1300ng / μl, 10μg of each sample is sent to the company for sequencing, and the two samples are resequenced on the Illumina GenomeAnalyzer sequencing system platform. Among them, about 32Gb double-end (pair-ends, PE) reads were obtained from the two samples respectively.

[0070] Then take Chinese cabbage and seaweed sprouts as F 1 , F 2There are 25 individual plants in each of the medium green and purple, and the extreme pools are constructed and then sequenced. Among them, the two mixed pools respectively obtain about 11Gb pair-ends (PE) reads. The genome of cabbage version 3.0 was selected as the reference genome, and bwa and samtools were used for SNP dev...

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Abstract

The invention discloses a molecule marker linked with a brassica compestris color gene of brassica compestris and an application of the molecule marker. The gene related to the brassica compestris color gene of brassica compestris is positioned at a No. 7 chromosome. A Chinese cabbage 3.0 edition is used as reference, and the gene insertion-deletion (Indel) is at a position between 25181252nd-25181356th from a 5' end of the No. 7 chromosome. The molecule marker disclosed by the invention can be used for identifying the brassica compestris or genotypes thereof, and further can be used for biological technology assisted breeding.

Description

technical field [0001] The invention relates to molecular assisted breeding, in particular to an InDel molecular marker linked with a porphyra sap color gene and its use. Background technique [0002] Cabbage vegetables (Brassica rapa L.) belong to the Brassica genus (Brassica) of the Cruciferae (Cruciferae). Including Chinese cabbage, Chinese cabbage, turnip, cabbage and other subspecies and varieties; it is one of the most widely distributed and planted vegetable crops in China, and occupies a pivotal position in agricultural production. Laver stalk and Chinese cabbage are two different cabbage crops. The veins and leaves of laver stalk are purple while the leaves of Chinese cabbage are green. This is mainly caused by the different anthocyanin content in the two different cabbage crops. [0003] With the gradual improvement of living standards, people pay more and more attention to the nutritional quality of vegetables. Anthocyanins and flavonol glycosides, as important ...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/6895C12Q1/686
CPCC12Q1/686C12Q1/6895C12Q2600/13
Inventor 王晓武程锋武剑张鑫张亢
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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