50 results about "Common gene" patented technology

The invention discloses a method for producing transgenic mice through a homologous recombination technology. The method comprises the following steps: replacing mouse NGF genes on mouse chromosomes by human NGF genes through a Cas-9/CRISPR gene knock-in technology, and obtaining the genes with homozygous human NGF genes through breeding and knocking the genes in mice, thus obtaining filial generation mice with salivary glands capable of secreting human NGF. Compared with the conventional targeting technology utilizing positive and negative double screening homologous recombination genes, the method disclosed by the invention is simple to operate, capable of being realized only by transfecting mouse embryonic stem cells by three plasmid DNAs or carrying out pronucleus injection on the zygotes of mice, and high in success rate which is up to 2-5% and remarkably higher than the mouse embryonic stem cell positive rate of 0.1% of the common gene targeting technology.

The invention provides a preparation method of organoid spheres. The preparation method comprises the steps that matrigel containing cells and fluorocarbon oil are introduced into a three-way device to obtain cell spheres, and after culture is performed, the organoid spheres are formed; according to the preparation method of the organoid spheres, the micro-fluid control method is adopted for generating mono-disperse biomaterial organs, high-throughput production is achieved, the sizes, shapes and structures of organoids are controllable and uniform, and compared with a 2D tumor sensitivity predicting model, the organoids are combined with tumor micro-environment factors, and the prediction result is more accurate; compared with a PDX mouse tumor sensitivity predicting model, the modeling period is shorter, and the cost is lower; compared with common gene sequencing, the clinic predicting rate is higher; the preparation method of organoid spheres has the important significance and valuefor clinic application, and the important basis is supplied to detection of related results.

A drug-resistance gene film chip for detecting mycobacterium tuberculosis is prepared by designing 54 probe spotting on a nylon film by aiming at the mutant sites of mycobacterium tuberculosis rpoB, kagG, embB, inhA, ahpC, gyrA, rrs, rpsL and pncA gene; 12 pairs of specific primers with biotin-labeled at 5' end are utilized; a sample DNA is subjected to triple PCR and amplified to form a large amount of gene fragment products with biotin; the amplified products and the probes on the film chip carry out specific hybridization; and then film washing, enzyme-linking and color reaction are carried out, thus preparing the chip. The gene film chip and the detection method thereof can detect the common gene mutations of the mycobacterium tuberculosis on the drug resistance of drugs such as isoniazid, rifampicin, streptomycin, ethambutol, pyrazinamide, quinolone and the like at one step, and are applicable to extracorporeal detection sputum sample, clinical isolation strains and mycobacterium tuberculosis multi-drug resistant gene in the organization sample.

The invention relates to a detection method of germs in soybean paste, in particular to a detection method of germs in traditional zymotic soybean paste, solving the problem that the prior PCR-DGGE technology can not accurately detect the germs in the traditional zymotic soybean paste. The detection method comprises the following steps: (1) the DNA extraction of a germ common gene group of a traditional zymotic soybean paste sample; (2) a V3 section of 16S rDNA of PCR amplified germs; and (3) the PCR-DGGE of the germs. The method of the invention thoroughly clear up other contents of the traditional zymotic soybean paste except microbial cells while avoiding the loss of the species and the amount of microorganism whenever possible, and the whole detection process does not change the DNA content, reduces the final analysis bias and ensures the precision of a detection result.

The invention provides a detection method for thyroid cancer related gene fusion mutation and a kit and relates to the technical field of gene engineering. The detection method comprises the followingsteps: performing detection, namely extracting total RNA (ribonucleic acid) or miRNA (micro ribonucleic acid) from a detection sample; performing reverse transcription on the total RNA or mRNA of thesample so as to obtain cDNA (complementary deoxyribonucleic acid), and performing fusion gene target specific PCR (polymerase chain reaction) amplification by using fusion gene target specific PCR amplification primers; performing electrophoresis detection on specific PCR amplification products so as to obtain types and sizes of fragments of the amplification products; and according to electrophoresis results, judging whether sample genes have thyroid cancer related gene fusion mutation or not. By adopting the method provided by the invention, three types of common gene fusion in thyroid cancer can be simply, conveniently and rapidly detected at an RNA level, and the method is high in sensitivity and applicable to clinical use.

The invention discloses a kit for detecting pathogenic bacteria of mucor lungs as well as a use method and an application of the kit. The kit of the present invention comprises a universal primer capable of being present in the form of a mixture, wherein the universal primer is capable of binding to a common gene conserved region of rhizopus oryzae, rhizomucor micranthum, aureobasidium pullulans, rhizopus stolonifer, and Cunninghamia lanceolata. The kit provided by the invention can realize one-time detection of various mucor fungi, the detection time does not exceed 2 hours, and the specific type of mucor can be further determined in one-time detection.

The invention belongs to the technical field of zymomonas mobilis, and discloses a method and an element for screening different-intensity ribosome binding sites and promoters. The method comprises the following steps: firstly, screening genes with strong downstream expression according to different genomics data, carrying out Wayne analysis, and screening common gene of the genomics data; predicting the binding site sequence of ribosome with different intensities; acquiring an RBS sequence promoter with specific intensity; acquiring a double-fluorescence reporter gene system framework; acquiring recombinant plasmid; acquiring a strain of the double-fluorescence reporter gene system plasmid containing the promoter with specific RBS intensity; and performing intensity verification. The method can integrate existing systematic biological data, and quickly and efficiently screen promoters with specific intensity. The intensities of the RBS and the promoter which are predicted and screenedby the method have good correlation with experimental data (respectively is R2)0.9 and R2)0.7).

The invention provides a standard substance for extensive oncogene detection. The standard substance includes 13 mutation sites verified by Droplet Digital PCR (ddPCR) and 500X high-throughput whole exome sequencing verified site information, has more than 700 variation sites of over 330 genes, also includes common structural variations of cancer genomes, such as common gene SNV, base insertion deletion and the like, has corresponding mutation site frequencies within a range of 1% to 100%, and is wide in application scene and platform. The standard substance can be used for evaluating stability, specificity and sensitivity of the working flow from sample extraction to biological information analysis, evaluating each sample treatment method and detecting performance differences among platforms. The standard substance belongs to a major innovation in the industry, and has wide application prospect and great industrial application value.

The invention provides 16 common genes for detecting a human natural killer cell immunoglobulin-like receptor KIR, wherein the 16 common genes comprise 14 KIR functional genes (2DL1, 2DL2, 2DL3, 2DL4,2DL5A/B, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3 and 3DS1) and 2 pseudo genes (2DP1 and 3DP1), and a combination of a PCR amplification primer group and a Taqman probe of the common variant ofthe 2DS4; and the combination comprises six primer groups. The invention also provides a kit containing the combination of the PCR amplification primer group and the Taqman probe. The invention has the following technical effects that: an ARMS analysis method is combined with a Taqman multiplex fluorescence PCR technology to carry out qualitative typing detection on the KIR gene; furthermore, improvement is carried out on the basis of a traditional ARMS; various defects of an existing detection method are overcome; and the method disclosed by the invention has wide application prospects and clinical reference value.

The invention provides a crRNA molecule and a method for detecting D614G mutation in SARS-CoV-2 by using CRISPR-Cas12a. The method for detecting D614G mutation in SARS-CoV-2 by using the CRISPR-Cas12a technology through the crRNA is simple, convenient, easy to implement, rapid and specific; when a recombinase polymerase nucleic acid amplification technology is combined, the sensitivity is 100 copies/[mu]L, the sensitivity is extremely high, compared with a common gene sequencing method, the method can achieve the identification of a sample with lower virus content, and is suitable for large-scale screening.

The invention discloses a genome segment filling method and device based on a segment contiguous group. The method comprises the following steps: calculating to obtain a deletion gene; classifying the maximum deletion gene string; merging the maximum deletion gene strings meeting the conditions; searching a Type-1 type string, and executing a Type-1 string insertion algorithm; searching for a Type-3-II type string without slot, and executing a no-slot-Type-3-II string insertion algorithm; searching Type-2 and Type-3 type strings, processing a common adjacency relation related to the contradictory common gene, and executing Type 2& 3-string insertion algorithm. Herein, the calculation is carried out based on the fragment contigs, the form is more general, and application is wider; the filling method is high in search speed and high in filling efficiency, can reduce the time and space complexity of genome segment filling based on the segment contiguous group, and improves the sensitivity and specificity of filling.

The invention relates to a group of nucleotide sequences for detecting the gene mutants of HBV, which comprise at least one of the nucleotide sequences from SEQ ID No.1 to SEQ ID No.15. The invention also provides the use of the sequences in detection of the gene mutants of HBV. When the nucleotide sequences are used, 10 common gene mutation loci of HBV can be detected. When used for detecting the gene mutants of the HBV, the nucleotide sequences have the advantages of high throughput, high sensitivity and high specificity, are low in detection cost and have a practical clinic promotion and application value.

The embodiment of the invention provides a drug action mechanism analysis method and device and electronic equipment, and relates to the technical field of computers. The method comprises the following steps: determining common gene targets related to a to-be-analyzed drug and a target disease treated by the to-be-analyzed drug; determining a target regulatory pathway between the gene targets in the common gene targets; constructing a first analysis network about the corresponding relationship among the target regulatory pathway, the common gene target and the target compound in the to-be-analyzed drug; and according to the common gene target represented by the first analysis network and the connectivity of the target compound in the to-be-analyzed drug, determining the effective components in the to-be-analyzed drug and the target gene target acted by the to-be-analyzed drug when the to-be-analyzed drug treats the target disease. Compared with the prior art, by applying the scheme provided by the embodiment of the invention, the action mechanism of the traditional Chinese medicine compound can be efficiently and systematically analyzed so as to comprehensively screen the effective components in the traditional Chinese medicine compound and the gene targets on which the effective components act.

The invention discloses a primer construction method for detecting hepatic echinococcosis through ddPCR, an amplification primer and application of the amplification primer. The primer construction comprises the following steps that common gene segments of echinococcus granulosus and echinococcus multilocularis are screened, and a plurality of first primers are designed based on the common gene segments; a plasma cfDNA sample of a first patient group is amplified by adopting the first primers, and the first primers capable of amplifying the plasma cfDNA sample of the first patient group are taken as second primers; and a plasma cfDNA sample of a second patient group is detected by adopting ddPCR of a reaction system comprising the second primers, and the second primers with the positive detection rate greater than a preset value are screened out as a target amplification primer. The amplification primer can amplify DNA fragments of echinococcus which are released into peripheral blood and exist in the form of plasma free DNA, by combining with a ddPCR method, whether human plasma is infected with echinococcosis can be diagnosed by detecting the human plasma, so that the ultra-early diagnosis of echinococcus granulosus and echinococcus multilocularis is realized, and a detection tool which is sensitive enough is provided for research and development of an echinococcosis precise therapy.