55results about How to "Visualization of test results" patented technology

The invention belongs to the field of nucleic acid detection and discloses a method for semi-quantitatively detecting pathogenic nucleic acid by adding internal control nucleic acid. Corresponding internal control is added in the whole process of extracting and amplifying target nucleic acid and testing by using a test paper, so that the internal control and a target segment are parallelly operated, and the semi-quantitative detection is performed finally through color development and intensity contrast of three strips, namely a detection line, an internal control line and a quality control line on the test paper. In the method, in the whole process of processing the target nucleic acid, the corresponding internal control is taken as a positive contrast, and false negative results due to links such as extraction, amplification or sample application errors are avoided in the processing of detecting by using the test paper. Meanwhile, by comparing color development intensity of the internal control line and a sample line and introducing the semi-quantitative function on the basis of the qualitative function of the immunochromatographic test paper to estimate the copy number of tested samples, the detection results are more detailed, accurate and reliable. The method has the advantages of convenient and quick operation and capacity of meeting the actual clinical requirement.

The invention discloses a sow side-lying posture real-time detection system based on joint partitioning of sow key parts and environment, and the system comprises a delivery room, a camera, a video storage unit, and a server, and the delivery room is used for placing a to-be-delivered sow; the camera monitors and obtains video data of a delivery room, continuously stores the video data to the video storage unit on one hand, and is directly connected with the server on the other hand; the server calls the backup video data and analyzes the monitoring data in real time; the working steps of thedetection system are as follows: monitoring the postures of the sows in real time, simultaneously detecting three areas of an approval area, a lactation area and a delivery area through a convolutional neural network area identification model, identifying the sows as lying postures when more than two areas are simultaneously detected, and outputting an identification result to a database. Comparedwith a method for recognizing the posture of the sow through a sensor technology, the computer vision technology avoids contact with the sow, stress response is reduced, and the method has the advantages of being low in cost and high in efficiency.

The invention discloses adapter modified nano silver. A nano silver ball with the diameter of 10nm to 30nm is used as a kernel; and the outer surface of the silver ball is bonded with an adapter chain and an oligonucleotide chain. The invention also discloses a preparation method of the adapter modified nano silver, a reagent kit comprising the adapter modified nano silver, and application of the adapter modified nano silver to preparation of a reagent for detecting IgE (Immunoglobulin E). When used for carrying out the detection of IgE, the nano silver material provided by the invention has high sensitivity, short detection time (about 2h to 3h), simple and feasible operation and visual detection result; downhole semi-quantitative detection can be achieved in a visual mode without the need of an especially expensive detection apparatus; and the adapter modified nano silver has low cost, can realize bulk detection, and is suitable for popularization and utilization.

The invention provides a photon crystal gel material for detecting glucose, which comprises polyacrylamide-acrylic acid gel and a photon crystal embedded in the gel, wherein the gel is directly or indirectly chemically grafted with phenylboronic acid groups, and the photon crystal is two-dimensional photon crystal or three-dimensional photon crystal. The invention also provides a glucose detection method based on the photon crystal gel material, and the color information displayed after the photon crystal gel material detects a glucose solution is converted into glucose concentration information. The glucose detection method provided by the invention can qualitatively or quantitatively detect glucose concentration with high selectivity, sensitivity, convenience and visualization, and provide a novel non-invasive detection method for urine glucose monitoring of diabetics.

The invention discloses a rubber plug quality detection device and method. The detection device at least comprises a uniform speed rotating platform, a detection position, a focus-fixing camera, a photoelectric switch, a light source, an industrial personal computer, a display device and a defective good processing device. Rubber plugs to be detected are conveyed to the detection position through the uniform speed rotating platform. When the photoelectric switch identifies that the rubber plugs to be detected are in the detection position, five focus-fixing cameras are triggered to conduct multi-angle shooting on the rubber plugs to be detected, the rear industrial personal computer conducts radius detection processing, roundness detection processing, trimming condition detection processing, thickness detection processing, impurity pot detection processing and other detection processing on the shot and collected rubber plug images, and data statistics, storage and display transmission are conducted on the detected results; the detected defective goods are removed. By means of the rubber plug quality detection device and method, comprehensive automatic quality testing, quality detection, result display and removal processing are finished in one detection device, the precision is high, the speed is high, efficient and reliable functions are achieved, the production cost is low, and the defects generated by manual detection are overcome.

The invention relates to a rape transgenosis detecting kit in the field of detecting articles. The rapeseed transgenosis detecting kit comprises a primer combination and a membrane chip, and is characterized in that the primer combination is a 11-double-starting oligonucleotide primer combination, the membrane chip refers to 11 groups of probe sequences and one group of positive control probe sequences, which are fixed on the surface of a supporting film, and the 5' end of each probe is provided with an amino marker; asymmetric PCR (polymerase chain reaction) amplification primers, which are Tag primers with biotin marks at 5'ends, are included in the primer combination. The rapeseed transgenosis detecting kit provided by the invention overcomes the defects of time and energy waste and high cost in the prior art, the rape transgenosis detecting kit provided is simple to operate, is quick and sensitive, is high in detection flux, has visual detection results and is low in cost.

The invention discloses a device and method for measuring the evenness of an LED area array light source, belongs to the field of LED area array light source measuring, and aims to solve the problems that currently a point selecting testing method is used to measure the LED area array light source, the accuracy of the evenness measuring is hard to guarantee, and a large amount of manpower, material resources and time needs to be consumed when the point selecting testing method is used to adjust the LED area array light source. The device comprises a straight-line motion mechanism, a detecting device, an analog signal collector and a control system. The device has the advantages that the light energy of the LED area array light source in the whole irradiating area can be scanned and detected in a short time, and the device is fast in detection, high in efficiency, accurate in processing result and visual in detecting result.

The invention discloses a livestock component detection kit and method and relates to the technical field of meat product detection. The kit comprises one or a combination of more of the following primer pairs: SEQ ID NO. 1-2, SEQ ID NO. 3-4, SEQ ID NO. 5-6, SEQ ID NO. 7-8, SEQ ID NO. 9-10, SEQ ID NO. 11-12, SEQ ID NO. 13-14, SEQ ID NO. 15-16, SEQ ID NO. 17-18, SEQ ID NO. 19-20, SEQ ID NO. 21-22,SEQ ID NO. 23-24 and SEQ ID NO. 25-26. When adopted to detect livestock source components, the kit has relatively high specificity and sensitivity and is accurate and reliable in detection result.

The invention discloses a kit for detecting genetically modified soybean in the field of test items. The kit for detecting genetically modified soybean comprises a primer combination and a membrane chip. The kit for detecting genetically modified soybean is characterized in that the primer combination is a seven polymerase chain reaction (PCR) primer combinations; each pair of primers comprise Pat, Bar, Cry1Ab, Cry105, EPSPS, P35S and a soybean endogenous gene sequence Lectin; the membrane chip is seven groups of probe sequences, which are orderly fixed on the surface of a support membrane and a group of positive control probe sequences, the probe sequences are respectively a Pat probe, a Bar probe, a Cry1Ab probe, a Cry105 probe, an EPSPS probe, a P35S probe, a Lectin probe and a Positive probe; an asymmetric PCR amplification primer is formed in the primer combination, and is a Tag primer with a biotin marker (biotin) at the 5' end. By adopting the kit for detecting genetically modified soybean, the defects of time and labor waste, and high cost in the prior art are overcome; the provided kit for detecting genetically modified soybean is simple to operate, quick and sensitive, large in detection throughput, visual in detection result and low in cost.

The invention provides a method for detecting hydrogen peroxide based on a bimetallic Co / Mn-MOFs enzyme-like property colorimetric method and belongs to the technical field of environmental monitoring. The method comprises: synthesizing two-dimensional bimetallic Co / Mn-MOFs through a one-step hydrothermal method. Through the synergistic catalysis effects of two metals in the material and a large specific surface area and accessible active sites of the two-dimensional structure, the Co / Mn-MOFs have good enzyme-like activity. When H2O2 exits, the material can promote decomposition of H2O2 so that a hydroxyl radical .OH is produced and the .OH oxidizes a chromogenic substrate TMB into oxTMB and thus the reaction system is changed from colorless to blue. The colorimetric sensing method has a H2O2 linear detection range of 1 to 100 micromoles per liter and a detection limit of 0.85 micromoles per liter. The method has the characteristics of simpleness, visuality, low cost and high sensitivity and provides a novel idea for use of the material in environmental monitoring and biological analysis.

The invention relates to a microbial fluorescent staining solution and an application thereof. The microbial fluorescent staining solution comprises lectin, fluorescein, nucleic acid dye, a buffer solution, an anti-quenching agent, a bacteriostatic agent and water; the microbial fluorescent staining solution can be used in microbial dyeing, specific recognition of agglutinin on glycoprotein and specific staining of nucleic acid by nucleic acid dye are utilized, according to the invention, the fungi are blue, green or red, the bacteria are red, the morphology is combined to achieve the detection on the microorganisms in a vaginal secretion sample, a cervical exfoliated cell sample, a skin sample and a sputum sample, the detection result can be visualized, and the microorganism variety can be directly and reasonably judged.

The invention belongs to the technical fields of bioelectrochemistry and biochip sensors and relates to an electrochemical biochip sensor array for mycobacterium tuberculosis 16SrDNA and for detecting mycobacterium tuberculosis and a preparing method thereof. Starting with molecular level detection of the mycobacterium tuberculosis 16SrDNA, Fe3O4@SiO2 composite nanometer particles and a nanometer Au structure material are adopted as mediators, a novel and functionalized nanometer electrochemical biochip superparamagnetism nano-detection microsphere sensor interface is designed, the mycobacterium tuberculosis is subjected to double enrichment extraction and purification detection, and the electrochemical biochip sensor array for rapidly detecting the mycobacterium tuberculosis is constructed. A clinical sample containing the mycobacterium tuberculosis is directly added into a magnetic reaction micropore. A mycobacterium tuberculosis 16SrDNA specific sequence can be subjected to double enrichment and biological stabilization through utilizing a capture probe labeled by the Fe3O4@SiO2 composite nanometer particles and a nanometer Au labeled detecting probe respectively. A high mycobacterium tuberculosis target molecule separating efficiency and high detection sensitivity are expected to be achieved by utilization of nano-detection microsphere superparamagnetism. In addition, target molecule "on-chip" separation is expected to be achieved through characteristics of the electrochemical biochip sensor array without the need of extra separating steps, thus largely simplifying operation procedures and shortening the detection time.

The invention discloses a nucleic acid probe combination, a kit and a method for detecting common pathogens in genital tracts. The nucleic acid probe combination comprises one or more groups of multiplex specific primer combinations and / or one or more probe combinations sequentially fixed on the surface of a membrane chip. The primer combination comprises primers of common pathogens in genital tracts and primers of endogenous control GAPDH genes. The probe combination comprises a specific probe for common pathogens in genital tracts, a positive dot hybridization probe and an endogenous control GAPDH gene probe. According to the invention, a multiplex PCR and reverse dot hybridization combined technology is adopted to solve problems of low assay accuracy of conventional primers and probes on pathogens in genital tracts and difficulty in realizing parallel assay of single reaction systems for more than seven pathogens in genital tracts. The nucleic acid probe combination of the present invention overcomes defects of easiness in false positive result caused by cross contamination and low accuracy in multiplex reaction systems in the prior art, and has the advantages of quick detection, convenience in operation, high accuracy and sensitivity, good specificity and visible detection results.

The invention discloses a rapid, simple, specific and sensitive detection kit suitable for most laboratories for detecting Candida albicans based on RPA (recombinase polymerase amplification). The kitcomprises a pair of primers and a probe, the nucleotide sequences of the primers are shown as SEQIDNO.1-2, and the nucleotide sequence of the probe is shown as SEQIDNO.3. The kit has two forms: a real-time fluorescence detection kit and a test strip kit. Experiments show that the kit is short in candida albicans detection time and high in sensitivity and specificity, the cost is further saved bycombining a simple nucleic acid crude extraction method, a new technical reference can be provided for field and nursing point detection, and the kit is of great significance in the aspect of pathogenic bacteria detection in areas lack of resource basic equipment.

The invention discloses an RPA primer, reagent and kit for fast detecting BLV (bovine leukemia virus). The RPA primer is characterized in that the DNA sequence of an upstream primer is <210>2; the DNAsequence of a downstream primer is <210>3. An RPA-LFA technology is used for building a BLV infection visual fast detection system; through the detection of a BLV nucleic acid sample, the result proves that the screened BLV RPA visual detection primer has high specificity and sensitivity. Compared with the prior art, the invention provides a fast, simple, convenient and accurate detection reagentfor the bovine BLV infection; particularly, the advantages of low requirement on the instrument equipment, need of only one constant-temperature water bath pot, fast detection speed (about 40 min), visual detection result, direct observation by naked eyes and the like are realized.

The invention discloses the establishment of a visual rapid detection method of a Bartonella henselae genome DNA, and belongs to the technical field of biology. A pair of high-specificity and strong-sensitivity RPA primers, namely an upstream primer (210)2 and a downstream primer (210)3, are screened, and an RPA (recombinase polymerase amplification) detection system of the Bh genome DNA is established. Compared with the conventional PCR method, the RPA-LFA method has the advantages of low requirements on instrument equipment (only one thermostat water bath is needed), high detection speed (about 40min), visualization of a detection result (the detection result can be directly observed by naked eyes) and the like, so that because of the advantages, the RPA-LFA method provided by the invention can be applied to rapid detection and diagnosis of cat Bh infection in a pet hospital.

The invention relates to a novel detection kit for transgenic aspergillus oryzae and a detection method thereof, and relates to the technical field of transgenic microbiological detection. By designing a labeled primer and a probe for specific sequences of transgenic aspergillus oryzae and combining PCR amplification with nucleic acid test strip detection technology, the invention realizes accurate, high-efficient and rapid detection of transgenic aspergillus oryzae. The method has strong specificity, high sensitivity and good stability, and plays an important role in improving accuracy and shortening detection time for transgenic aspergillus oryzae detection. The detection kit is convenient and rapid for use, simple in principle and operation, and applicable to rapid transgenic aspergillus oryzae detection.

The invention discloses a lens module detection method and a lens module detection system, which are used for quickly and conveniently detecting whether the position of a lens module in assembled projection equipment is qualified or not. The lens module detection method comprises the steps of acquiring an image captured by means of the lens module on a calibration image, determining a first picture area Pa1, determining a second picture area Pa2 in the captured image, determining a positional relationship between a calibration mark and the second picture area in the image, and judging whetherthe lens module is qualified. The lens module detection method and the lens module detection system utilize the picture captured from the calibration image for processing and detection, feeds back data to the interface, is simple in the implementation operating process, and can quickly and effectively complete the detection of assembled products; hardware modules of the lenses do not need to be detected in actual detection, the detection result is visualized, the lens module detection method and the lens module detection system can be applied to production quality detection of various lens modules, improve the efficiency of equipment factory delivery detection, can effectively screen out the bad finished products in factory delivery, improve the use of equipment and enhance the user experience.

The invention provides an RPA (recombinase polymerase amplification) reagent and device for detecting antibiotic resistance genes and a detection method of the RPA reagent and device. The invention discloses the RPA reagent for detecting antibiotic drug resistance genes. The antibiotic drug resistance genes are blaNDM, blaKPC, mcr and tetLR, and the reagent comprises a reagent A for detecting the blaNDM drug resistance gene, a reagent B for detecting the blaKPC drug resistance gene and a reagent C for detecting the mcr drug resistance gene; the kit further comprises any one of a reagent D for detecting a tetL drug-resistant gene and a reagent E for detecting a tetR drug-resistant gene. According to the reagent, the device and the detection method provided by the invention, a normal-temperature amplification mode is adopted to amplify and detect the antibiotic drug resistance gene, the dependence of large-scale instruments and equipment can be completely eliminated, portable miniaturization and mobility are realized, and the requirement of quick detection in environmental sample detection is met.

The invention discloses primer groups, a kit and a method for detecting a specific sequence of a glyphosate-resistant transgenic soybean transformant. Two groups of LAMP (loop-mediated isothermal amplification) primers are designed, the primer group 1 is used for detecting an internal reference gene Lectin and can detect soybean ingredients in a sample and simultaneously exclude false negative results, and the primer group 2 is used for detecting the specific sequence of the glyphosate-resistant transgenic soybean transformant, has high specificity and can effectively detect glyphosate-resistant transgenic soybean ingredients, wherein the sequence is a boundary sequence which connects an inserted exogenous gene with a plant gene. When the kit is used for the detection and analysis of a sample, a positive control, a blank control and an internal control are respectively set, so that the production of false positives and false negatives can be avoided. Special equipment is not required for detection, and the detection cost is low; and the detection results can be judged by visual observation of changes in color of a reaction solution, the identification is simple and convenient to perform, the results are visualized, and the primer groups, the kit and the method disclosed by the invention are suitable for field fast detection and grassroots screening of a large number of samples and easy to realize large-range popularization and application.

The invention belongs to the field of biotechnology detection, and particularly provides a Prader-Willi Syndrome detection kit and a use method thereof. According to the present invention, snO-lncRNA1, snO-lncRNA2, snO-lncRNA3, snO-lncRNA4 and snO-lncRNA5 are extremely highly expressed in human embryonic stem cells, and abnormally stable, so that the detection kit prepared by taking snO-lncRNA1, snO-lncRNA2, snO-lncRNA3, snO-lncRNA4 and snO-lncRNA5 as detection genes can detect samples such as common blood, saliva, urine and the like so as to provide guarantee for realizing non-invasive examination of Prader-Willi Syndrome; the use method is simple to operate and has low requirements on instruments and equipment; the input amount of the required sample is small, and the sample source is highly inclusive; the kit has the characteristics of high sensitivity, high specificity and the like; the detection result is visual, simple and easy to understand; and by using the detection method, Prader-Willi Syndrome can be detected in a short time.

The invention relates to a bovine-derived component detection kit, and belongs to the technical field of detection articles, the bovine-derived component detection kit consists of a primer combinationand a membrane chip, and is characterized in that the primer combination is a four-fold PCR primer combination, each pair of primers is a yellow cattle D-loop, a buffalo D-loop, a yak D-loop and an endogenous gene sequence 16 S rRNA, the membrane chip comprises four groups of probe sequences and a group of positive control probe sequences which are sequentially fixed on the surface of a supporting membrane, wherein the probe sequences are a yellow cattle D-loop probe, a buffalo D-loop probe, a yak D-loop probe, a 16 S rRNA probe and a Positive probe respectively; an asymmetric PCR amplification primer is arranged in the primer combination, which is the primer with a biotin marker at the 5' end. The bovine-derived component detection kit overcomes defects of time and labor waste and high cost in the prior art, and the operation of the provided bovine-derived component detection kit is simple, fast and sensitive, the detection flux is large, the detection result is visible and the costis low.

The invention provides a primer for detecting metapneumovirus. Nucleic acid detection of the metapneumovirus is carried out by specifically amplifying an N gene of C-type metapneumovirus. The invention further provides an metapneumovirus detection kit, visualization of a detection result is achieved through RTPCR amplification, nanogold tracing and double signal amplification, the kit is used for metapneumovirus detection, the detection lower limit can be greatly reduced, and meanwhile the specificity, sensitivity, accuracy and repeatability of virus detection are effectively improved. When the kit is used for detecting metapneumovirus, the pretreatment process of a sample is simple, consumed time is short, and a large number of samples can be detected simultaneously.

The invention discloses a split type visualized multi-path signal intelligent detector which comprises a signal detector sending end 5, a signal detector receiving end 6 and a detection socket 7. Thesignal detector sending end 5 comprises a multivibrator 1, a decade counter 2, a power supply 3, a pair of signal cable plugs and multiple LED lamps 4; the multivibrator 1 provides counting pulses forthe decade counter 2; the power supply supplies power to the detection device; the decade counter 2 provides signals for the LED lamps 4; and signal cables to be detected are connected to the detection socket 7. A detection result is visualized and is more visual and clear; the flickering frequency of the LED lamps is adjustable, and use is more human-based; the split design is not limited by theusing distance; a fault occurrence position can be determined intelligently, and convenience is provided for maintenance; and a present loading control system is used, so that test loading is stableand controllable.

The present invention discloses specific LAMP primers, kit and method for detecting streptococcus uberis. The kit comprises the six LAMP primers including outer primers F3 and B3, inner primers FIP and BIP and loop primers LB and LF, and the six primers aim at conserved sequence design of a streptococcus uberis KX389960. 1 gene; and a nucleotide sequence of FIP is as shown in SEQ ID NO.1, a nucleotide sequence of BIP is as shown in SEQ ID NO.2, a nucleotide sequence of F3 is as shown in SEQ ID NO.3, a nucleotide sequence of B3 is as shown in SEQ ID NO.4, a nucleotide sequence of LB is as shownin SEQ ID NO.5, and a nucleotide sequence of LF is as shown in SEQ ID NO.6. The kit solves the problems that an existing breast streptococcus detection technology has the long period and the high detection cost and cannot be applied to on-site rapid detection.

The invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. According to the technical scheme, the invention discloses a PCR (Polymerase Chain Reaction) high-resolution melting detection kit for 15 animal-derived components and a detection method. Through primer optimization screening, three pairs of universal primers are adopted for combined detection, the melting temperature Tm of species is used as a characteristic value to establish a standard library, and 15 animal-derived components of cattle, buffalo, yak, goat, sheep, rabbit, horse, donkey, pig, dog, cat, mouse, fox, mink and raccoon dog in meat and meat products can be rapidly, simply and conveniently detected. The method is simple, convenient and rapid, agarose electrophoresis is not needed, cross contamination is avoided, and corresponding specific primers do not need to be designed for each species; by referring to PCA and LDA analysis methods in statistics, the detection result is more visual; compared with various traditional PCR technologies, the method has the advantages that the problem of competition among primers is solved, the detection efficiency is greatly improved, and the reaction cost is reduced.

The invention discloses an RPA primer and a probe for detecting shell parasitic perkinsus. Sequences of the primer are shown as SEQ ID NO.1 and SEQ ID NO.2, a sequence of the probe is shown as SEQ IDNO.3, a biotin marker is added to 5' terminal of a downstream primer R1, an FAM marker is added to 5' terminal of the probe, a C3Spacer marker is added to 3' terminal, and dSpacer or THF is added intothe probe for modification. The invention further discloses a kit comprising the primer and the probe, application of the primer and the probe in detecting shell parasitic perkinsus and a method of adopting an RPA process to detect shell parasitic perkinsus. The primer, the probe, the kit and the method are short in detection time, high in sensitivity and specificity, low in requirement on operation environment and wide in application range.

The invention discloses an acetone gas detector based on charge transfer and a detection method thereof, wherein the detector comprises a gas input channel, a response cavity, ultraviolet diodes, a MoTe2 sensitive layer, micro metal electrodes, gold wires, a display screen, a current sampling chip and a gas output channel. The MoTe2 sensitive layer generates electron hole pairs under the excitation of the ultraviolet diodes; when the organic gas acetone adsorbs, the electron hole pairs are separated, and a large amount of electrons are transferred to acetone molecules, causing the current to rise sharply; and when other organic gases adsorb, only a small amount of electron holes are transferred to the organic gas molecules, so that the resulting change in the current can be substantially negligible. According to the acetone gas detector based on charge transfer and the detection method thereof, the specific detection of acetone in the mixed organic gas is achieved with the high sensitivity and specific selection for acetone.

The invention provides a method for detecting hydrogen peroxide based on a bimetallic Co / Mn-MOFs enzyme-like property colorimetric method and belongs to the technical field of environmental monitoring. The method comprises: synthesizing two-dimensional bimetallic Co / Mn-MOFs through a one-step hydrothermal method. Through the synergistic catalysis effects of two metals in the material and a large specific surface area and accessible active sites of the two-dimensional structure, the Co / Mn-MOFs have good enzyme-like activity. When H2O2 exits, the material can promote decomposition of H2O2 so that a hydroxyl radical .OH is produced and the .OH oxidizes a chromogenic substrate TMB into oxTMB and thus the reaction system is changed from colorless to blue. The colorimetric sensing method has a H2O2 linear detection range of 1 to 100 micromoles per liter and a detection limit of 0.85 micromoles per liter. The method has the characteristics of simpleness, visuality, low cost and high sensitivity and provides a novel idea for use of the material in environmental monitoring and biological analysis.