Specific LAMP primers, kit and method for detecting streptococcus uberis

Abstract

The present invention discloses specific LAMP primers, kit and method for detecting streptococcus uberis. The kit comprises the six LAMP primers including outer primers F3 and B3, inner primers FIP and BIP and loop primers LB and LF, and the six primers aim at conserved sequence design of a streptococcus uberis KX389960. 1 gene; and a nucleotide sequence of FIP is as shown in SEQ ID NO.1, a nucleotide sequence of BIP is as shown in SEQ ID NO.2, a nucleotide sequence of F3 is as shown in SEQ ID NO.3, a nucleotide sequence of B3 is as shown in SEQ ID NO.4, a nucleotide sequence of LB is as shownin SEQ ID NO.5, and a nucleotide sequence of LF is as shown in SEQ ID NO.6. The kit solves the problems that an existing breast streptococcus detection technology has the long period and the high detection cost and cannot be applied to on-site rapid detection.

Description

technical field [0001] The invention relates to the field of microorganism detection, more specifically, to a specific LAMP primer, a kit and a method for detecting Streptococcus uberis. Background technique [0002] Streptococcus uberis (Streptococcus uberis) is one of the most common pathogens causing mastitis in dairy cows. It can cause clinical and subclinical mastitis in cows, cause chronic infection of mammary gland tissue, and is highly contagious, eventually leading to low milk production in cows. decline or even stop lactation. Seriously restrict the development of dairy farming and dairy processing, and endanger public health and human health. [0003] Usually, the detection of Streptococcus uberis in clinical samples is still through classical methods such as bacterial isolation, biochemical tests and CAMP tests. However, due to the shortcomings of classic methods such as cumbersome operation, long time-consuming, and low detection rate, it affects the rapid and...

AI Technical Summary

Problems solved by technology

However, due to the shortcomings of classic methods such as cumbersome operation, long time-consuming, and low detection rate, it affects the rapid and accurate detection of the bacteria and the treatment of mastitis caused by it; the existing polymerase chain reaction (polymerase chain reaction, PCR ) technologies include ordinary PCR, multiplex PCR, real-time fluorescent quantitative PCR, etc., among which these methods have high specificity and sensitivity, but require expensive instruments and equipment, and have high technical requirements for testing personnel, making them unsuitable for grassroots popularization and application

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