The invention aims at providing a
reagent for assisting the identification of arabis
mosaic virus and an application thereof. The
reagent provided by the invention comprises a specific primer consisting of
DNA (Deoxyribonucleic Acid) shown in a sequence 1 in a sequence table,
DNA shown in a sequence 2 in the sequence table, and
DNA shown in a sequence 3 in the sequence table. The
reagent also comprises a specific probe shown in a sequence 4 in a
nucleotide sequence table. The arabis
mosaic virus is an important
quarantine pest in China, and a method for detecting ArMV by semi-nested-RT (
Reverse Transcriptase)-Realtime PCR (
Polymerase Chain Reaction) is established by utilizing three nested type PCR primers and a
TaqMan Probe. According to the method, the nested PCR technology and the real-time
fluorescence PCR technology are organically combined; two PCR systems formed by the three primers and the probe are verified with each other, so that the result accuracy is effectively improved; the detection flexibility is effectively improved by the real-time
fluorescence PCR technology; and the accuracy, the flexibility, the simplicity, the convenience and the rapidness are realized, and the minimum
detection limit can reach 0.5fg / mul
plant total RNA (Ribonucleic Acid).