GeXP multiple rapid detection primers for detection of bluetongue virus, bovine viral diarrhea virus and foot and mouth disease virus and detection method

Abstract

GeXP multiple rapid detection primers for detection of bluetongue virus, bovine viral diarrhea virus and foot-and-mouth disease virus and a detection method are provided; the detection method for various cow diseases based on a GeXP multiple gene expression genetic analysis system is intended to be built up; the problems that when detection of the various cow diseases, a serological method has low sensitivity, conventional PCR only detects single pathogens and a gene chip method has relatively high cost are solved; three cow disease DNA positive sample standard plasmids are constructed; a reference obtained by 10-time continuous dilution of the plasmids DNA is used as a detection object, and the detection sensitivity is 10-100 copies; no cross reactions with other cow diseases happen, and no false negative results exist; the number of detected actual simples is 600, and monitoring screening requirements of various diseases of a lot of cows are met.

Description

technical field [0001] The invention relates to the application field of biotechnology, in particular to the simultaneous detection and identification of three imported cow virus diseases. Background technique [0002] Foot and mouth disease (Foot and Mouth Disease, FMD), bovine viral diarrhea (Bovine Viral Diarrhea, BVD) and bluetongue (Bluetongue, BT) are three viral diseases that require isolation and quarantine for imported dairy cows. In recent years, as the national demand for high-quality dairy products has increased, the number of imported dairy cows has increased year by year, which has brought a certain amount of work pressure and financial burden to animal disease testing laboratories at dairy cow entry ports. Therefore, it is of great significance to establish a high-throughput rapid detection method that can simultaneously differentially diagnose multiple dairy cow diseases to reduce the workload of the inspection laboratory at the port of entry of dairy cows, r...

AI Technical Summary

Problems solved by technology

Virus isolation and culture is the gold standard for diagnosis, but there are problems such as long test period and cumbersome operation; virus neutralization test requires specific standard serum or virus strain, which has certain limitations in practical application; imported enzyme-linked immunosorbent immunoassay Kits are generally expensive; molecular biology methods such as ordinary PCR and fluorescent quantitative PCR are all aimed at the detection of a single virus, which cannot meet the detection needs of differential diagnosis of mixed infections with multiple pathogens

Multiplex PCR technology has been applied to the differential diagnosis of various pathogens, but due to the problem of amplification preference, it is impossible to realize high-throughput detection in the true sense

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