The invention discloses a primer and a probe combination for detecting bovine viral diarrhea virus by a recombinase polymerase application (RPA) technology. The forward primer sequence is as shown in SEQ ID No.1; the reverse primer sequence is as shown in SEQ ID No.2; and the probe sequence is as shown in SEQ ID No.3. The invention also discloses a kit for bovine viral diarrhea virus detection. When the primer and the probe are used for detecting, a clinical sample only needs the treatment steps of virus RNA extraction, reverse transcription by a one-step method to form cDNA, and isothermal amplification, thermal cycle reaction is not needed, amplification does not need to be performed in a polymerase chain reaction (PCR) instrument, and the result can be displayed clearly on a lateral flow assay test strip, so that the primer and the probe have the advantages of high sensitivity, high specificity, simple reaction procedure, short detection time and the like, and are applicable to quick, accurate, simple and convenient diagnosis and treatment on the diseases in cattle farms.