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Kit and usage method thereof

A technology of kits and primer pairs, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as unreasonable primer design, STR grouping, low amplification efficiency, and poor typing effect

Inactive Publication Date: 2017-07-18
北京普利斯康医药技术有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0005] However, the existing STR genetic analysis technology has defects such as unreasonable primer design and STR grouping, complex methods for processing DNA templates, amplification systems, and poor reaction conditions, resulting in low amplification efficiency, long detection time, and poor typing results.

Method used

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  • Kit and usage method thereof

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Embodiment Construction

[0013] One aspect of the present invention relates to a kit, comprising a kit for amplifying STR loci D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, PentaD and Amelogenin One or more of the 16 pairs of upstream and downstream primers, the sequences of the 16 pairs of upstream and downstream primers are shown in SEQ ID NO: 1-32.

[0014] The kit of the present invention involves 16 pairs of upstream and downstream primers. The above 16 pairs of upstream and downstream primers have the advantages of good specificity and high amplification efficiency. Specific amplification and cross-reaction avoid the impact of non-target products on the typing results. At the same time, the amplification efficiency is high and the detection signal is strong. On the one hand, it can shorten the PCR amplification time. On the other hand, due to the high detection signal, it can be quickly Accurate detection of PCR products and STR typing...

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Abstract

The invention provides a kit and a usage method thereof. The kit comprises one or more pairs of 16 upstream and downstream primer pairs for amplifying STR loci, namely, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, vWA, Penta E, Penta D and Amelogenin, wherein the sequences of the 16 pairs of upstream and downstream primers are shown as SEQ ID NO:1 to SEQ ID NO:32. By adopting the kit and the usage method provided by the invention, the related 16 pairs of upstream and downstream primers have the advantages of high specificity and high amplification efficiency, and genotyping of the 16 STR loci can be performed rapidly and accurately.

Description

technical field [0001] The present invention relates to the field of STR detection, in particular to a kit and a method for using the same. Background technique [0002] Short tandem repeats (short tandem repeats, STRs), also known as microsatellite DNAs (microsatellite DNAs) or simple sequence repeats (simple sequence repeats, SSRs), are a type of tandem repeats with several bases as the core unit widely present in the human genome. The resulting DNA sequence. The STR locus is widely distributed, and there is one in every 100-200kb in the human genome on average, and its core repeating unit is generally 2-6 bases, and the number of repeats is between 10-60 times. Due to the difference in the number of repeating units between different individuals, there is a high polymorphism, and this polymorphism follows the Mendelian inheritance law in the genetic process, so STR genetic analysis is widely used in forensic individual identification, kinship identification, Population g...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156C12Q2600/16
Inventor 杨志伟杨雪艳李云
Owner 北京普利斯康医药技术有限公司
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