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A method and system for individual identification and paternity identification of unknown specimens

A technology of paternity identification and material inspection, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc. It can solve the problems that cannot meet the requirements of forensic DNA rapid inspection and on-site inspection ability, shorten the detection time, etc.

Active Publication Date: 2016-03-30
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing STR typing technology is limited by various factors (including the state of the sample material, primers, amplification sites, amplification conditions, and the amplification system used, etc.), and the PCR process alone takes more than 3 months. In the past few hours, scholars have conducted research on shortening the PCR amplification time by fast DNA polymerase, fast thermal cycler, microfluidic chip technology, etc., but none of them have achieved the ideal effect of shortening the detection time.
With the increase of emergencies, the existing detection system cannot meet the requirements of public security for the rapid detection and on-site detection of forensic DNA in actual combat

Method used

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  • A method and system for individual identification and paternity identification of unknown specimens
  • A method and system for individual identification and paternity identification of unknown specimens
  • A method and system for individual identification and paternity identification of unknown specimens

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Verification of the accuracy of the method and system for individual identification and paternity identification of unknown specimens of the present invention

[0059] In this example, the unknown specimens are 120 human fresh anticoagulated blood samples (60 males and 60 females). These specimens are samples collected by the applicant from Zhongguancun Community Hospital in Beijing, and their individual sources are known. , but in the implementation process of Example 1 of the present application, its individual source is assumed to be unknown, and the method and system of the present application are used to carry out individual identification and paternity identification, including:

[0060] 1) Utilize the DNA extraction system in the system of the present invention to extract the DNA of unknown specimens, 2) Utilize the composite detection system in the system of the present invention to obtain the DNA including 14 autosomal STR loci and 2 Y chromosome loci...

Embodiment 2

[0082] Example 2 Comparison of the system of the present invention for individual identification and paternity identification of unknown specimens with existing kits for individual identification

[0083] 120 parts of DNA samples in Example 1 were used to type 17 loci with the system of the present application (the typing process was the same as in Example 1, the difference being that the number of cycles of the amplification process was 28), while using the existing DNATyper15 TMThe kit (i.e. the conventional detection system) performs typing on the autosomal locus of the above samples (using the parameters of the kit itself), and the typing result is used as the positive control of the autosomal locus, Yfiler TM The kit performs typing on the Y chromosome locus (using the kit’s own parameters), and the typing result is used as a positive control for the Y chromosome locus, and a blank reagent is set as a negative control, and repeated 3 times in parallel.

[0084] The DNA t...

Embodiment 3

[0089] Embodiment 3 The sensitivity of the method and system result of the present invention

[0090] Get standard DNA sample 2ng, 1ng, 0.75ng, 0.5ng, 0.25ng, 0.125ng respectively, amplify and detect according to the method provided by the present invention, repeat 3 times in parallel, the result is as follows Figure 5 shown.

[0091] The results are shown in Table 4. It can be seen that the optimal amount of DNA template in the method of the present invention is between 0.5 and 2 ng. For samples with a DNA concentration greater than 0.5 ng / μL, the detection rate can reach 100%, which is slightly lower than the detection line. It is lower than the detection level of the STR detection kit (0.125ng) commonly used in the field of forensic science, but the amplification time of about 1 hour is significantly shorter than these conventional kits, which usually require about 3 hours of amplification time.

[0092] Table 4 DNA test results of sensitivity verification

[0093]

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Abstract

The invention provides a method and system for individual recognition and paternity identification of an unknown sample. The method includes: extracting the DNA of the unknown sample; acquiring the typing result of 17 loci contained by the DNA, i.e. 14 autosome STR loci, 2 Y-chromosome loci, and the sex determination locus Amelogenin, with the STR loci being D6S1043, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51 and FGA, the Y-STR locus being DYS448, and the Y-chromosome insertion / deletion site being M134; and carrying out individual recognition and paternity identification according to the genotypes of the 17 loci of the unknown sample. The invention also provides the system for individual recognition and paternity identification of the unknown sample. The scheme involved in the invention can realize simultaneous detection of autosome STR loci and Y-chromosome loci, and also can shorten the detection time, thus improving the efficiency of individual recognition.

Description

technical field [0001] The invention relates to a method and system for individual identification and paternity identification, in particular to a method and system for individual identification and paternity identification of unknown specimens. Background technique [0002] STR typing technology has become the core of the second-generation forensic DNA typing technology since the 1990s due to its advantages of good specificity, high detection sensitivity, and ability to combine multi-site amplification. However, the existing STR typing technology is limited by various factors (including the state of the sample material, primers, amplification sites, amplification conditions, and the amplification system used, etc.), and the PCR process alone takes more than 3 months. In the past few hours, scholars have conducted research on shortening the PCR amplification time by fast DNA polymerase, fast thermal cycler, microfluidic chip technology, etc., but none of them have achieved t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 李彩霞孙敬韩俊萍赵兴春刘鹏
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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