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294 results about "Y-Chromosome Genes" patented technology
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In general, the human Y chromosome is extremely gene poor—it is one of the largest gene deserts in the human genome, however there are several notable genes coded on the Y chromosome: not including pseudoautosomal genes, genes encoded on the human Y chromosome include:
Non-invasive detection of fetal genetic traits
InactiveUS20050164241A1Facilitates non-invasive detectionComponent separationOther chemical processesPregnancyNon invasive
Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≦500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≦500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.
Owner:SEQUENOM INC
System for identifying and sorting living cells
ActiveUS20120122084A1Bioreactor/fermenter combinationsMaterial analysis using sonic/ultrasonic/infrasonic wavesFlow cellIr absorption
In embodiments of the present invention, a system and method of cytometry may include presenting a single sperm cell to at least one laser source configured to deliver light to the sperm cell in order to induce bond vibrations in the sperm cell DNA, and detecting the signature of the bond vibrations. The bond vibration signature is used to calculate a DNA content carried by the sperm cell which is used to identify the sperm cell as carrying an X-chromosome or Y-chromosome. Another system and method may include flowing cells past at least one QCL source one-by-one using a fluid handling system, delivering QCL light to a single cell to induce resonant mid-IR absorption by one or more analytes of the cell, and detecting, using a mid-infrared detection facility, the transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a cell characteristic.
Owner:1087 SYST
Non-invasive detection of fetal genetic traits
ActiveUS20080071076A1Facilitates non-invasive detectionSugar derivativesOther chemical processesPregnancyNon invasive
Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains .Itoreq.500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of .Itoreq.500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising .Itoreq.500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.
Owner:SEQUENOM INC
High purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa
InactiveUS7371517B2Image distortionHigh refractive indexAnimal reproductionMaterial analysis by optical meansX chromosomeAnalysis method
Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).
Owner:XY
System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
InactiveUS7094527B2Promote divisionQuality improvementAnimal reproductionDead animal preservationX chromosomeBiology
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY
Separation Systems of Frozen-Thawed Spermatozoa Into X-Chromosome Bearing and Y-Chromosome Bearing Populations
InactiveUS20070042342A1Narrow range in magnitudeIncrease the differenceMicrobiological testing/measurementPreparing sample for investigationX chromosomeFreezing thawing
Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.
Owner:XY
Assay systems for detection of aneuploidy and sex determination
InactiveUS20120219950A1Improve throughputEasy to useMicrobiological testing/measurementAutosomal inheritanceChromosomal inheritance
The present invention utilizes detection of selected nucleic acid regions from pseudoautosomal regions to identify sex chromosomal aneuploidy and to determine fetal sex. Traditional methods of detecting sex chromosomal aneuploidies and performing sex determination typically involves some analysis of the Y chromosome. The assay systems of the present invention utilizing copy number variant detection of pseudoautosomal regions allows quantification of the sex chromosomes in mixed samples using loci that display autosomal inheritance patterns.
Owner:ROCHE MOLECULAR SYST INC
Statistical analysis for non-invasive sex chromosome aneuploidy determination
ActiveUS20130275103A1Increase contentBiostatisticsComputation using non-denominational number representationX chromosomeStatistical analysis
The present invention provides methods for non-invasive determination of X and / or Y chromosomal abnormalities indicative of aneuploidy or sex mosaicisms in a maternal sample by detecting and determining the relative contribution of genetic sequences from the X chromosome and / or the Y chromosome in the maternal sample.
Owner:ROCHE MOLECULAR SYST INC
In-Vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
ActiveUS20060281176A1Promote divisionQuality improvementAnimal reproductionDead animal preservationX chromosomeBiology
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
Owner:XY
Method for generating cloned animals using chromosome shuffling
InactiveUS20020174449A1Population uniformImprove quality controlNew breed animal cellsFermentationSpecific chromosomeMammal
The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with "autosomally isogenic" meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both "autosomally" and "allelically" isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.
Owner:ADVANCED CELL TECH INC
Method and system for noninvasive detection of fetus chromosome aneuploid
ActiveCN103525939AImprove scalabilityBirth rate controlMicrobiological testing/measurementSpecial data processing applicationsX chromosomeRelational model
The invention belongs to the medical detection field, and discloses a method and a system for noninvasive detection of fetus chromosome aneuploid. The disclosed detection method and system also relate to a method and a system for elimination of sequencing GC preference in chromosomes and among chromosomes and a method and a system used for the relation model of the Z values of X and Y chromosomes in a normal male fetus. Through elimination of influences of sequencing GC preference in chromosomes and among chromosomes, the relation model of the Z values of X and Y chromosomes in a normal male fetus is built, and the determination threshold of difference between the theoretical value and the actual value of the Z value of the X chromosome is built. The accurate detection of fetus chromosome aneuploid, especially sex chromosome aneuploid is achieved.
Owner:BOAO BIOLOGICAL CO LTD
Animal sex control method based on Rbmy gene editing and application
ActiveCN105861554AIncrease productivityNucleic acid vectorFermentationControl animalPlant Germ Cells
The invention discloses an animal sex control method based on Rbmy gene editing and application. The method controls animal sex by using CRISPR / Cas9 system to specifically cut Rbmy gene on Y chromosome. Rbmy gene encodes a germ cell specific nucleoprotein, this protein is an important factor related to spermatogenesis, and by cutting Rbmy gene, it is possible to deactivate Rbmy gene, thus Y sperm or fertilized ovum with Y sperm is deactivated and cannot develop into a normal embryo, birth ratio of female animals is finally increased and sex control is achieved. Therefore, sex ratio of animal offspring can be controlled through the method so that the ratio of female animals with better economic value is increased and production efficiency and economic effect are greatly improved.
Owner:SOUTH CHINA AGRI UNIV
Forensic medicine compound detection kit based on Y chromosome SNP (single nucleotide polymorphism) genetic marker
ActiveCN103131787AFast personal identificationGood polymorphismMicrobiological testing/measurementAlleleForensic science
The invention belongs to the field of forensic medicine genetics and in particular relates to a forensic medicine compound detection kit based on a Y chromosome SNP (single nucleotide polymorphism) genetic marker for individual recognition and genetic relationship identification by a legal medical expert. The forensic medicine compound detection kit provided by the invention is used for carrying out forensic medicine genetic relationship identification and individual recognition on human biology detection materials by utilizing a Y chromosome SNP genetic marker. According to the technical scheme for solving the technical problem, the forensic medicine compound detection kit based on the Y chromosome SNP genetic marker comprises a separated and packaged compound amplification primer mixture, a multiple single-basic-group extension reaction primer mixture, an allele typing standard mixture, a compound amplification reaction mixture and a single-basic-group extension reaction mixture. The kit provided by the invention can be applied to detection of common degradable materials in forensic medicine.
Owner:SICHUAN UNIV
Kit for multiplex amplification of 24 loci of human genome DNA
ActiveCN103614361AQuick checkImprove detection efficiencyMicrobiological testing/measurementDNA preparationHuman DNA sequencingBlood filter paper
The invention relates to a five-color fluorescence labeling multiplex amplification system for analysis of 24 loci of human genome DNA at the same time. 19 autosome loci, 4 Y chromosome loci and 1 sex determination locus are detected at the same time. The system divides the 24 loci into 4 groups, and relates to fluorescence labeling of 5 colors. The fluorescence labeling multiplex amplification system has high sensitivity, under condition that the DNA template amount is 0.12ng, all the 24 loci can be detected, and the system is suitable for direct amplification of blood filter paper and FTA card collecting samples.
Owner:AGCU SCIENTECH +1
Materials and methods for sperm sex selection
InactiveUS20090208977A1Strong specificityIncrease probabilityMammal material medical ingredientsArtificial cell constructsAntigenSemen sample
Materials and Methods for the separation of X- and Y-chromosome bearing sperm, for example in a semen sample, are provided. The methods involve contacting the semen sample with a binding agent, such as an antibody, that specifically binds to an antigen that is specific for an X- or Y-chromosome. Kits for use in the methods are also provided.
Owner:ANDROGENIX
Composite amplification kit for 26 Y chromosome short tandem repeats
ActiveCN104017895AHigh individual recognition rateImprove compatibilityMicrobiological testing/measurementDNA/RNA fragmentationAnalysis dnaTandem repeat
The invention relates to a composite amplification system for simultaneously analyzing multiple Y-STR loci. The composite amplification kit is characterized by compositely amplifying the following 26 Y chromosome loci: DYS19, DYS388, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a, DYS385b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, DYS460, DYS481, DYS533, DYS549, DYS570, DYS576, DYS643, DYS449 and Y-GATA-H4. The invention also relates to a method and a kit for simultaneously analyzing DNA samples, and applications thereof.
Owner:BEIJING PEOPLESPOT TECH
Method for determining genetic affiliation, substructure and gene flow within human populations
InactiveUS6929911B2Sugar derivativesMicrobiological testing/measurementDNA paternity testingPresent method
The present invention provides novel polymorphisms on the Y chromosome and methods of using these polymorphisms as well as known polymorphisms on the Y chromosome as indicators of evolutionary heritage. The polymorphisms of the present invention clustered to specific regions of the Y chromosome, and polymorphisms of particular use to the present methods are found in the non-recombining region of the human Y chromosome (NRY). These polymorphisms, including SNPs, insertions, and deletions, may be useful for numerous applications, including forensics, paternity testing, diagnosis and the like.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Multiple real-time fluorescent PCR detection kit for Y chromosome microdeletion
ActiveCN102703578ANo pollution concernsReal-time observation of experimental resultsMicrobiological testing/measurementY chromosome microdeletionFluorescence
The invention relates to the chromosome deletion detection field, and especially relates to a multiple real-time fluorescent PCR detection kit for Y chromosome microdeletion. The kit detects gene deletion at sY84 and sY86 loci of a Y chromosome AZFa subregion, sY127 and sY134 loci of a Y chromosome AZFb subregion, and sY254 and sY255 loci of a Y chromosome AZFc subregion by real-time fluorescent PCR. The kit realizes a stable and high-efficient multiple PCR reaction system by using homologous tailed primers and universal primers, adopts nucleic acid probes labeled by different fluorescent genes to indicate the presence of target fragments, and thus realizes the fastest simultaneous detection of sequences on 6 AZF deletion hotspots and 2 internally controlled genes by two tubes. The kit of the invention can be used for detection of male Y chromosome microdeletion genes, and has the advantages of high speed, accuracy, high throughput, low cost, no pollution, and the like.
Owner:郭奇伟
Amplification composite for detecting microdeletion of Y-chromosome and detection kit
ActiveCN102912028AReliable resultsThe test results are credibleMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceFluorescent pcr
The invention relates to amplification composite for detecting the microdeletion of Y-chromosome and a detection kit, belonging to the field of biotechnical detection. The amplification composite for detecting the microdeletion of Y-chromosome can be amplified to as many as 30 sites related to the microdeletion detection of the Y-chromosome through one reaction. The detection kit detects the microdeletion of the Y-chromosome through the quantitative fluorescent PCR (Polymerase Chain Reaction) method by using the amplification composite. The microdeletion abnormality of the Y-chromosome is determined according to the existence of an amplification product and the quantity of the amplification product. A large quantity of sites enables the detection result to be more convincible and can provide much more and more detailed information for determining the deletion type, and the quantitative detection of partial deletion and repetition can be realized. The detection kit is easier and more convenient to operate, only one PCR amplification and one sequencer detection reaction are needed to complete the detection of one sample, the whole process needs 4-5 h, and the operation intensity and the detection time are greatly reduced.
Owner:BEIJING MICROREAD GENE TECH
Probes, method and chip for detecting alpha and/or beta-thalassemia mutation based on whole-gene capture sequencing and application of such probes, such method and such chip
ActiveCN106591441AEnables detection of deletions in large regionsMicrobiological testing/measurementDNA/RNA fragmentationBeta thalassemiaNew mutation
The invention provides primers, a method and a chip for detecting alpha and / or beta-thalassemia point mutation and deletion mutation based on whole-gene capture sequencing and application of such primers, such method and such chip. The primers, the method, the chip and application thereof have the advantages that through designing of capture probes, relevant genes involved in alpha-thalassemia and beta-thalassemia are enriched and all mutation information including SNP and indel in full-length sequences of genes is detected; through addition of autosome, X-chromosome and Y-chromosome regions as well as upstream and downstream regions of coded genes as references, structure variations such as SNV and CNV are detected; compared with existing various hotspot mutation site detection technologies, the method is capable of detecting hotspot mutation information as well as some rare mutations and undiscovered new mutation types to detect and analyze full-length sequence specificity of target genes, fully covers the mutation types and makes up the defect that a conventional detection method easily causes missing detection of low-frequency mutations and rare mutations greatly.
Owner:SHENZHEN E GENE TECH
Markers of alterations in the Y chromosome and uses therefor
InactiveUS20060166228A1Bioreactor/fermenter combinationsBiological substance pretreatmentsInfertility treatmentsBiology
Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods of diagnosing or aiding in the diagnosis and / or cause of reduced sperm count and in methods of predicting or aiding in the prediction of the likelihood of success of infertility treatments.
Owner:WHITEHEAD INST FOR BIOMEDICAL RES
Compound amplification kit for InDel genetic polymorphic sites of human euchromosome and Y chromosome and application thereof
ActiveCN106868150AIncrease the number of detectionsShort ampliconMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceTyping
The invention discloses a compound amplification kit for InDel genetic polymorphic sites of human euchromosome and Y chromosome and application thereof. The kit comprises 47 pairs of euchromosome InDel site loca, 2 pairs of Y chromosome InDel site loca and a pair of specific amplification primers of a sex determination gene. The kit can be used for human individual recognition, paternity identification and degraded detection material recognition. The kit comprises 49 InDel sites which are relatively balanced in types and one sex determination gene. By adopting a six-colored STR fluorescence detection system, the kit is higher than the disclosed legal medical InDel detection kit. The kit disclosed by the invention is suitable for detecting Chinese groups. An amplification system of 50 sites is short in amplification fragment, and the amplicon is controlled at 200bp, so that the system is suitable for InDel typing of high degraded detection material; the amplification system improves the detection numbers of sites in the degraded detection material; the two Y-InDel sites introduced into the system plays an auxiliary judging role on the sex determination gene Amelogenin.
Owner:GUANGZHOU CRIMINAL SCI & TECH RES INST +2
Fluorescent labeling composite amplification kit for 30 STR (short tandem repeat) loci of human Y chromosome and application of kit
InactiveCN106148552AIncrease the number of testsRaise the possibilityMicrobiological testing/measurementFluorescenceY-STR
The invention discloses a fluorescent labeling composite amplification kit for 30 STR (short tandem repeat) loci of human Y chromosome and application of the kit. It is possible to multiple amplify 30 low-mutation-rate STR loci on Y chromosome in single reaction; by designing locus arrangements and specific primer sequences, 30 loci are divided into 5 groups, and six-color fluorescent system marks are used; the Y-STR kit is the domestically first one employing six-color fluorescent detection technology and is an STR kit detecting a highest number of loci, all the loci included are low-mutation-rate Y-STR loci, and the kit is more suitable for examining male families; the composite amplification kit is good in primer specificity, can tolerate a wide range of temperatures and is highly adaptive to subject materials.
Owner:AGCU SCIENTECH
Determination method of fetal DNA content in maternal plasma, based on single-nucleotide polymorphic loci
ActiveCN103215350AEasy to operateImprove accuracyMicrobiological testing/measurementY-Chromosome GenesBiology
The invention provides a determination method of fetal DNA content in maternal plasma, wherein the method is based on single-nucleotide polymorphic loci. The method comprises the steps such as single-nucleotide polymorphic loci screening, plasma locus DNA extraction, PCR amplification reaction, fetal DNA amount calculation, and the like. The operation method of the method is simple. Amplification based on single-nucleotide polymorphic loci is adopted, such that the difficulty of determination of fetal DNA content in plasma of pregnant women with female fetus due to the application of Y chromosome genetic locus marker is avoided. The method is widely applied, and has high accuracy.
Owner:SUZHOU BASECARE MEDICAL DEVICE CO LTD
Fluorescence-labeled composite amplification kit for Y chromosome STR (short tandem repeat) gene loci capable of improving distinguishing capability and application thereof
ActiveCN102703583AEasy to identifyHigh polymorphismMicrobiological testing/measurementFluorescenceCommercial kit
The invention provides a fluorescence-labeled composite amplification kit for Y chromosome STR (short tandem repeat) gene loci capable of improving distinguishing capability. When the kit is used for detecting DNA (deoxyribonucleic acid) gene, not only can 17 STR gene loci of DYS391, DYS389I / II, DYS439, DYS438, DYS456, DYS458, DYS437, DYS635, DYS448, Y-GATA-H4, DYS19, DYS392, DYS393, DYS390 and DYS385a / b, which can be analyzed by the commercial kit, be amplified and analyzed, but also at least one of STR gene loci of DYS449, DYS527a / b, DYS522, DYS388, DYS447 and DYS444 can be simultaneously amplified and analyzed, so that the accumulative individual distinguishing capability and cumulative probability of exclusion of the system are improved, and the individual distinguishing capability is improved overall.
Owner:AGCU SCIENTECH
Detection method for Y chromosome micro-deleted gene
InactiveCN101575647AMeet the experimental conditionsSimple adjustment of primer concentrationMicrobiological testing/measurementMultiplex pcrsY-Chromosome Genes
The invention discloses a detection method for Y chromosome micro-deleted gene. In the method, after human peripheral blood genome DNA is extracted, multiple PCR primer design is used, wherein a multiple PCR reaction system comprises N pairs of chimeric primers for specifically amplifying the STSs site in an AZF segment of a Y chromosome and one pair of universal primers. N+1 pairs of primers are participated in the multiple PCR reaction, and the reactions of DNA template such as denaturalization, anneal and extending in circulating amplification target are carried out in a same reaction system; 10ulPCR products are taken and dye by EB, and electrophoresis is carried out through 3% agarose. The electrophoresis voltage is 4 V / cm, and the electrophoresis time is 20 minutes; and then the electrophoretogram is observed under an ultraviolet transilluminator so that the result is judged. Therefore, a detection method with simplified PCR reaction conditions, high amplification efficiency and easy operation for clinic experiments is established.
Owner:成都军区昆明总医院
A method for detecting sexual development related gene variation and a kit therefor
ActiveCN105734120AComprehensive assessmentImprove throughputBioreactor/fermenter combinationsBiological substance pretreatmentsX chromosomeSolid substrate
The invention discloses a kit. The kit comprises a probe. The probe is fixed to a solid substrate or is free in a liquid phase. The probe can specifically recognize exon regions of specific 29 genes of an X chromosome and at least another region of the X chromosome. A distance, on the X chromosome, of any two adjacent regions in the regions specifically recognized through the probe of the X chromosome is not more than 10 M. The probe can specifically recognize an exon region of an SRY gene of a Y chromosome and at least another region of the Y chromosome. A distance, on the Y chromosome, of any two adjacent regions in the regions specifically recognized through the probe of the Y chromosome is not more than 10 M. In addition, the invention also discloses uses of the kit in detection of sexual development related genes, a method of detecting SRY gene variation, a device for detecting the SRY gene variation and a method of detecting sexual development related gene variation.
Owner:天津华大基因科技有限公司 +1
Specific molecular markers of sex chromosomes of Tilapia nilotica and genetic sex identification method
ActiveCN101962641AEconomic divisionQuick distinctionMicrobiological testing/measurementDNA/RNA fragmentationX chromosomeNile tilapia
Owner:SOUTHWEST UNIVERSITY
Composite amplification kit for 47 human autosome and Y chromosome loci and application thereof
ActiveCN109750110AEffective expansionAmplification specificMicrobiological testing/measurementDNA/RNA fragmentationDNA paternity testingFluorescence
The invention relates to a composite amplification kit of 47 human autosome and Y chromosome loci and an application thereof. The invention provides a composite amplification system of the 47 human autosome and Y chromosome loci, which comprises specific primers for amplifying the 47 loci, wherein the 47 loci comprise 19 autosome STR loci, 27 Y chromosome STR loci and 1 sex recognition locus. The47 pairs of specific primers are subjected to grouping fluorescence labeling by utilizing a six-color fluorescence labeling technology, and the simultaneous high-efficiency, specific and sensitive amplification of the 47 human autosome and Y chromosome loci is achieved through the design and optimization of primer sequences and working concentrations. The detection result of the composite amplification system has high individual identification capability and good data compatibility, and can be used for paternity identification and individual identification in practice, so that the detection cost of human DNA typing is effectively reduced, and the detection working efficiency is improved.
Owner:BEIJING PEOPLESPOT TECH
Multiplex amplification detection kit for detecting 60 loca of autosomes and Y-chromosomes simultaneously
ActiveCN109439765ARapid expansionImprove balanceMicrobiological testing/measurementCapillary electrophoresisMagnetic bead
The invention discloses a multiplex amplification detection kit for detecting 60 loca of autosomes and Y-chromosomes simultaneously. The 60 loca are amplified in a multiplex manner through a polymerase chain reaction, and amplification products of the loca are detected by using a gene sequencer. The kit can be used for detecting the 60 loca of the autosomes and the Y-chromosomes simultaneously, which is a case that most STR loca can be detected by a primary reaction with a capillary electrophoresis method at present, and databases can be built for autosome STR and Y-chromosome STR simultaneously by the primary reaction. The kit has strong adaption to biomaterials, namely, one kit can be used for amplifying various biomaterial samples, wherein different biomaterial samples comprise a male genome DNA extracted by a Chelex100 method, a magnetic bead extracting method or an organic extracting method and male blood or oral cells of human, which is / are collected by any one carrier of filterpaper, an FTA card, a cotton bud, gauze and the like. The kit has higher amplification specificity and higher thermal tolerance.
Owner:江苏苏博生物医学科技南京有限公司 +1
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