294 results about "Y-Chromosome Genes" patented technology

In embodiments of the present invention, a system and method of cytometry may include presenting a single sperm cell to at least one laser source configured to deliver light to the sperm cell in order to induce bond vibrations in the sperm cell DNA, and detecting the signature of the bond vibrations. The bond vibration signature is used to calculate a DNA content carried by the sperm cell which is used to identify the sperm cell as carrying an X-chromosome or Y-chromosome. Another system and method may include flowing cells past at least one QCL source one-by-one using a fluid handling system, delivering QCL light to a single cell to induce resonant mid-IR absorption by one or more analytes of the cell, and detecting, using a mid-infrared detection facility, the transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a cell characteristic.

Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains .Itoreq.500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of .Itoreq.500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising .Itoreq.500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.

Isolated non-naturally occurring populations of spermatozoa (15) having high purity and technologies to differentiate spermatozoa (28) based on characteristics such as mass, volume, orientation, or emitted light including methods of analysis and apparatus such as beam shaping optics (30) and detectors (32).

Devices, compositions, and methods for handling, separating, packaging, and utilization of spermatozoa (1) that can be derived from previously frozen sperm samples collected from a male mammal. Specifically, techniques to uniformly stain (2) spermatozoal DNA even when derived from previously frozen sperm and separation techniques to separate and isolate spermatozoa even when derived from previously frozen sperm samples into X-chromosome bearing and Y-chromosome bearing populations having high purity.

The present invention utilizes detection of selected nucleic acid regions from pseudoautosomal regions to identify sex chromosomal aneuploidy and to determine fetal sex. Traditional methods of detecting sex chromosomal aneuploidies and performing sex determination typically involves some analysis of the Y chromosome. The assay systems of the present invention utilizing copy number variant detection of pseudoautosomal regions allows quantification of the sex chromosomes in mixed samples using loci that display autosomal inheritance patterns.

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with "autosomally isogenic" meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both "autosomally" and "allelically" isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.

The invention relates to the chromosome deletion detection field, and especially relates to a multiple real-time fluorescent PCR detection kit for Y chromosome microdeletion. The kit detects gene deletion at sY84 and sY86 loci of a Y chromosome AZFa subregion, sY127 and sY134 loci of a Y chromosome AZFb subregion, and sY254 and sY255 loci of a Y chromosome AZFc subregion by real-time fluorescent PCR. The kit realizes a stable and high-efficient multiple PCR reaction system by using homologous tailed primers and universal primers, adopts nucleic acid probes labeled by different fluorescent genes to indicate the presence of target fragments, and thus realizes the fastest simultaneous detection of sequences on 6 AZF deletion hotspots and 2 internally controlled genes by two tubes. The kit of the invention can be used for detection of male Y chromosome microdeletion genes, and has the advantages of high speed, accuracy, high throughput, low cost, no pollution, and the like.

The invention relates to amplification composite for detecting the microdeletion of Y-chromosome and a detection kit, belonging to the field of biotechnical detection. The amplification composite for detecting the microdeletion of Y-chromosome can be amplified to as many as 30 sites related to the microdeletion detection of the Y-chromosome through one reaction. The detection kit detects the microdeletion of the Y-chromosome through the quantitative fluorescent PCR (Polymerase Chain Reaction) method by using the amplification composite. The microdeletion abnormality of the Y-chromosome is determined according to the existence of an amplification product and the quantity of the amplification product. A large quantity of sites enables the detection result to be more convincible and can provide much more and more detailed information for determining the deletion type, and the quantitative detection of partial deletion and repetition can be realized. The detection kit is easier and more convenient to operate, only one PCR amplification and one sequencer detection reaction are needed to complete the detection of one sample, the whole process needs 4-5 h, and the operation intensity and the detection time are greatly reduced.

Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods of diagnosing or aiding in the diagnosis and / or cause of reduced sperm count and in methods of predicting or aiding in the prediction of the likelihood of success of infertility treatments.