85 results about "Analysis dna" patented technology

Processes are disclosed that use 3′-reversibly terminated nucleoside triphosphates to analyze DNA for purposes other than sequencing using cyclic reversible termination. These processes are based on the unexpected ability of terminal transferase to accept these triphosphates as substrates, the unexpected ability of polymerases to add reversibly and irreversibly terminated triphosphates in competition with each other, the development of cleavage conditions to remove the terminating group rapidly, in high yield, and without substantial damage to the terminated oligonucleotide product, and the ability of reversibly terminated primer extension products to capture groups. The presently preferred embodiments of the disclosed processes use a triphosphate having its 3′-OH group blocked as a 3′-ONH₂ group, which can be removed in buffered NaNO₂ and use variants of Taq DNA polymerase, including one that has a replacement (L616A).

A biometric mobile device is capable of interacting with existing cellular, wireless, and wired telecommunication and other communication networks to support intelligence gathering, human body identification, special operations and other applications. A method of collecting biometric data at an accident or crime scene may comprise, for example, utilizing a camera to photograph the accident scene, collecting key entered data that may not be otherwise obtainable, using a fingerprint scanner to collect, digitize and store fingerprint data, using a lab-on-a-chip DNA profile device for collecting and analyzing a DNA specimen and generating identification and DNA profile data for bar code entry and other means for collecting any known form of biometric data including, but not limited to, vascular facial structure, dental structure, cornea, iris or other data which may be unique or limiting for identification purposes.

The invention disclosed herein improves upon existing tissue microarray technology by using frozen tissues embedded in tissue embedding compound as donor samples and arraying the specimens into a recipient block comprising tissue embedding compound. Tissue is not fixed prior to embedding, and sections from the array are evaluated without fixation or post-fixed according to the appropriate methodology used to analyze a specific gene at the DNA, RNA, and/or protein levels. Unlike paraffin tissue arrays which can be problematic for immunohistochemistry and for RNA in situ hybridization analyses, the disclosed methods allow optimal evaluation by each technique and uniform fixation across the array panel. The disclosed arrays work well for DNA, RNA, and protein analyses, and have significant qualitative and quantitative advantages over existing methods.

Provided is a method for obtaining human DNA for genetic analysis, by taking the epidermis of testee by means of an adhesive sheet, and by extracting DNA from the epidermis stuck on the adhesive sheet. Provided are also combined sheets for conveniently storing DNA and a kit for taking the epidermis and analyzing DNA. Along with the kits, the method allows DNA to be easily obtained and stably stored for a long period of time. In addition, both the identification and the DNA analysis of a testee can be conducted at the same time by taking epidermal scraps from the testee, along with a figured epidermal print.

The present invention relates to a microfluidic device for extracting and isolating DNA from cells. The device includes a support having an inlet port for receiving a sample containing a cell, an outlet port for dispensing DNA isolated from the cell, and a microfluidic channel disposed within the support and extending from the inlet port to the outlet port. The microfluidic channel includes a micropillar array, an inflow channel disposed between the inlet port and the micropillar array, and an outflow channel disposed between the micropillar array and the outlet port. The micropillar array includes micropillars spatially configured to entrap, by size exclusion, the cell, to immobilize DNA released from the cell, and to maintain the immobilized DNA in elongated or non-elongated form when hydrodynamic force is applied to the microfluidic channel. Systems and methods of making and using the device are also provided herein.

The present invention to provides a solution to problems associated with the use of hybridization for genetic analysis, including but not limited to the use of microarray technology for the analysis DNA. The present invention provides compositions and methods for the use reflections of DNA in genetic analysis. The present invention is also directed to methods for the production of reflections of DNA.

A system, methods, and apparatus are described to collect and prepare single cells and groups of cells from microsamples of specimens and encode spatial information of the physical position of the cells in the specimen. In some embodiment, beads or surfaces with oligonucleotides containing spatial barcodes are used to analyze DNA or RNA. The spatial barcodes allow the position of the cell to be defined and the nucleic acid sequencing information, such as target sequencing, whole genome, gene expression, used to analyze the cells in a microsample for cell type, expression pattern, DNA sequence, and other information, in the context of the cell's physical position in the specimen. In other embodiment, markers such as isotopes are added to a microsample to encode spatial position with mass spectoscopy or other analysis. The spatial encoded information is then readout by analysis such as DNA sequencing, mass spectrometry, fluorescence, or other methods.

The invention relates to a compound amplification system for simultaneously analyzing a plurality of STR (short tandem repeat) loca, which is characterized by being used for carrying out compound amplification on 20 locas: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. The invention also relates to a method and a kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and other applications of the loca.

Reagents and methods for analysis of DNA hydroxymethylation are provided. Methods comprise modification of hydroxymethylated cytosine residues with a bulky moiety to protect hydroxymethylated positions from cleavage with a DNA endonuclease. For example, methods may comprise contacting DNA with a glucosyltransferase to glucosylate hydroxymethylated DNA positions and digesting the DNA with a DNA endonuclease to cleave DNA in positions lacking hydroxymethylation. Reagents and kits for hydroxymethylated DNA analysis are also provided.

This disclosure describes a device for obtaining a blood sample from an intruder at a crime scene to be later analyzed for DNA. A projectile containing a blood-extracting needle for penetrating the skin of said intruder is mounted through a protective chamber and connected to a blood reservoir in the chamber interior. A vacuum is held in both the reservoir and the chamber. The chamber vacuum is released by forward movement of the chamber as it slides within a casing following further penetration of the needle. The vacuum release acts to reduce the ambient pressure within the forward portion of the casing, thus assisting in propelling the needle into the intruder. As the needle reaches maximum extension, a loaded spring within the casing is released, propelling the needle and reservoir away from the intruder.

Methods of analyzing DNA to identify regions of the genome that are methylated in a genomic sample are disclosed. In one aspect genomic DNA is fragmented using a restriction enzyme with a degenerate recognition site, methylated restriction fragments are separated from unmethylated fragments by affinity purification. The complexity of the methylated fragments is reduced by amplification of a subset of the fragments using adaptors that ligate to a subset of the fragments. The amplified product is fragmented, labeled and hybridized to an array of probes. The hybridization pattern is analyzed to determine methylation status of cytosines.

Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers.

The present invention provides an analysis apparatus including: an irradiation section for irradiating the chromatin structure with terahertz waves; a detection section for acquiring a set of terahertz wave spectral information from the chromatin structure; a memory section for memorizing the sets of terahertz wave spectral information corresponding to the states of the chromatin structure; and a data processing section for analyzing the state of the chromatin structure by comparing the set of spectral information acquired in the detection section and the sets of spectral information memorized in the memory section.

The present invention relates to compositions, methods and systems for analyzing the methylation state of nucleic acids. Some embodiments relate to a compositions, methods and systems for analyzing the methylation state of DNA with a gene array.