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Compound amplification kit of 20 short tandem repeats

A technology of compound amplification and compound amplification system is applied in the field of simultaneous amplification of multiple short tandem repeat loci and detection of genetic marker genes with polymorphism in the human genome, which can solve the waste of DNA database data resources and the effectiveness not enough

Active Publication Date: 2010-09-01
BEIJING PEOPLESPOT TECH +1
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AI Technical Summary

Problems solved by technology

Therefore, due to the different loci of each STR kit, some data resources of the DNA database are wasted, and the effectiveness is not enough

Method used

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  • Compound amplification kit of 20 short tandem repeats
  • Compound amplification kit of 20 short tandem repeats
  • Compound amplification kit of 20 short tandem repeats

Examples

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Embodiment 1

[0045] Example 1 Compound amplification of 20 gene loci and analysis of their genotypes

[0046] Blood is donated by volunteers. Template DNA was extracted from blood by chelex-100 method. The amplification reaction was carried out on the ABI 9700 thermal cycler, the electrophoresis and detection were carried out on the ABI 3100 genetic analyzer, and the data analysis was performed using GeneMapper ID v3.2 software. The samples were detected by SinoFiler kit from ABI company and Goldeneye 16A kit from Genepoint Cognitive Co., Ltd. The operation method was carried out according to the instructions of the kits, and the results were used as a control. The reagents and materials used in the present invention, such as allelic ladder (ladder), are conventional materials commonly used by those skilled in the art.

[0047] 1.1. chelex-100 method to extract DNA (specific method refer to "Forensic DNA Protocol", Humana Press, 1998)

[0048] 1) Take 3 μl of blood with anticoagulant in...

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Abstract

The invention relates to a compound amplification system for simultaneously analyzing a plurality of STR (short tandem repeat) loca, which is characterized by being used for carrying out compound amplification on 20 locas: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. The invention also relates to a method and a kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and other applications of the loca.

Description

Technical field: [0001] The present invention relates to the field of biotechnology. Further, the present invention relates to the detection of polymorphic genetic marker genes in the human genome. In particular, the present invention relates to the simultaneous amplification of multiple short tandem repeat loci in one amplification system using polymerase chain reaction. Background technique: [0002] Short tandem repeats (STR for short), also known as microsatellites or simple sequence repeats (SSR), are a type of DNA tandem repeats widely present in eukaryotic genomes, with a core sequence of 2-6 base repeat units. The number of STR loci is large, the division is extensive, accounting for about 3% of the entire genome (International Human Genome Sequencing Consortium, 2001), and the polymorphism is high, and its polymorphism is mainly derived from the difference in the number of repeats of the core sequence between individuals. And this difference follows the Mendelian ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q2600/16C12Q1/6876
Inventor 高阳陈初光马斌王曙光
Owner BEIJING PEOPLESPOT TECH
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