77 results about "Short Tandem Repeat Profile" patented technology

This invention provides compositions and methods for genetic testing of an organism and for correlating the results of the genetic testing with a unique marker that unambiguously identifies the organism. The markers may be internal markers, such as for example single nucleotide polymorphisms (SNPs), short tandem repeats (STRs), or other sites within a genomic locus. Alternatively, the markers may be external, such that they are separately added to the genetic sample before testing.

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, i.e. aneuploidy. In addition, the present invention provides methods to determine when there are insufficient fetal cells for a determination and report a non-informative case. The present invention involves quantifying regions of genomic DNA from a mixed sample. More particularly the invention involves quantifying DNA polymorphisms from the mixed sample.

The invention relates to a composite amplification system of 23 short tandem repeat sequences and a kit, is used for detecting heredity mark gene of polymorphism in a mankind genome, and belongs to the biology technical field. The invention relates to a scheme that a plurality of short tandem repeat sequences are simultaneously amplified in a PCR system; a specific gene locus comprises 22 short tandem repeat sequences with high level heredity polymorphism and 1 gender determination locus; primers are respectively designed and a fluorophore is marked; the system has very high individual recognition rate and non-father elimination rate; the system and kit can be used for legal medical individual recognition, paternity tests, and colony genetics analysis, is high in accuracy, and good in sensitivity.

The invention discloses a method for non-invasive preimplantation hereditary detection of embryos, and belongs to the technical field of biological detection. The method comprises the following steps:carrying out whole genome amplification on a blastocyst culture solution sample by using a kit, carrying out short tandem repeat sequence analysis on an amplification product and DNA samples of parents to detect maternal pollution, carrying out library preparation and next-generation sequencing detection on the amplification product to determine whether the number of chromosomes is abnormal or not; and optimizing a pre-amplification mixed solution and an amplification mixed solution by the provided kit. According to the method provided by the invention, the blastocyst culture solution can besubjected to parent source pollution detection, so that whether the chromosome aneuploidy detection result of the culture solution is accurate and reliable or not is judged. The invention provides a detection method for judging whether granular cells are completely removed or not, and the inhibition effect of components in the culture solution on amplification is effectively avoided through optimization of the kit, so that amplification uniformity is good, and the single cell amplification yield is high. The detection method is simple, the result is accurate, and data quality is improved.

The application discloses an accurate human DNA typing method, a reagent and application. According to the accurate human DNA typing method, a short tandem repeat sequence, polynucleotide polymorphicsites covering a whole genome, a mitochondrial DNA hypervariable region I, a mitochondrial DNA hypervariable region II and an Amel enamel gene are detected simultaneously to obtain accurate typing andbase sequences of all the sites, thereby realizing accurate typing of human DNAs. The disclosed method having high resolution ratio is capable of carrying out efficient and accurate individual recognition and has the extremely high individual recognition capacity for difficult detection materials and highly-degraded detection materials; the non-parent exclusion rate is larger than 99.999999% in paternity identification and the accuracy is high; the compatibility is high, the existing individual recognition detection kit can be covered, and the method can be used for analyzing race groups in different regions or countries by combining human genome data. Besides, the method disclosed by the invention is relatively high in adaptability of detection materials and the human DNA extracted by various experimental methods can be detected.

The invention provides a multiplex amplification kit of 18 short tandem repeats (STR), relating to a multiplex amplification system which simultaneously analyzes a plurality of STR loca. The multiplex amplification kit is characterized by carrying out multiplex amplification on the following 18 loca: Amelogenin, D6S477, GATA198B05, D15S659, D8S1332, D3S3045, D17S1290, D14S608, D2S441, D18S535, D13S325, D10S1435, D11S2368, D1S1656, D7S3048, D10S1248, D19S253 and D3S1358. The invention also relates to a method and kit for simultaneously analyzing DNA (deoxyribonucleic acid) samples and applications thereof.

The invention provides a preparation method of a placenta source matrix mesenchymal stem cell. The preparation method comprises the following steps: (1) separating the placenta source matrix mesenchymal stem cell; (2) cultivating the placenta source matrix mesenchymal stem cell, wherein a trypLE<TM> enzyme solution is utilized to process a placenta tissue in two steps in the step (1), and a serum-free medium is utilized in the step (2). By adopting the method provided by the invention, the placenta source matrix mesenchymal stem cell is a complete matrix source by short tandem repeat sequence (STR) atlas analysis, and is qualified by chromosome karyotype examination, pathogenic microorganism examination of bacteria, funguses and viruses, cell purity identification and cell biology function identification. Therefore, the placenta source matrix mesenchymal stem cell obtained by the method provided by the invention does not contain foreign protein, can meet the requirements of clinical use, is especially suitable for use by mothers, and also provides a technical support for building an excellent placenta source matrix mesenchymal stem cell bank.

The invention relates to a composite amplification system and a detection kit of a mouse short tandem repeat and belongs to the technical field of biotechnology. The invention relates to mouse short tandem repeats (D2Mit395.1, D4Mit170.1, D5Mit201.1, D7Mit40, D11Mit4, D15Mit16 and D17Mit51.1) and a sex determination site (Jarid1) for simultaneously amplifying mice in a PCR (polymerase chain reaction) system, carrying out composite amplification and capillary electrophoresis detection on mouse DNA (deoxyribonucleic acid). The system can be used for genotyping of a mouse cell, each site adopts fluorescence-labeled primers, so that an obtain product is provided with a fluorescence label, and the detection can be carried out on an apparatus such as a genetic analyzer, so that the obtain segment length is more exact, and higher in repeatability. The system is simple to operate and convenient for large-scale popularization, the whole process has automation equipment, and the whole detectiontime only needs about 6 hours.

The invention provides a new STR (short tandem repeat, Short tandern repeat,) preparation method of allele ladder (Allele ladder, AL), which comprises extracting DNA from human nucleated cells, and synthesizing corresponding to these STRs The primers of the site, select the DNA of individuals with different alleles, use PCR technology to specifically amplify the corresponding amplification products, use HPLC to purify and separate the amplified products, and perform PCR amplification→purification→PCR for multiple cycles Amplification→purification, and finally the purified products with different alleles at each site are mixed in equal proportions to form each STRAL. The present invention also provides a typing kit with the allele ladder prepared by this method. The preparation method and typing kit described in the invention can be used in aspects such as individual identification, paternity identification and gene diagnosis in the fields of forensic science, anthropology, genetics and diseases.

In a method of deducing the number of repeat units in a selected short tandem repeat (STR) in a genomic sample, at least a single stranded target DNA generated from a genomic sample comprising a selected STR, an STR probe (P1,P1′), a reference probe (P2), and two blockers (B1,B2) are provided, and at least three differential hybridization experiments are carried out, based on which the number of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in each differential hybridization experiment is determined. The method further comprises the step of comparing these numbers of STR probe oligonucleotides (P1,P1′) bound per target DNA strand in the differential hybridization experiments for deducing the number of repeat units in the selected STR on the single stranded target DNA strand. Also disclosed are kits for carrying out STR genotyping by differential hybridization.

The invention relates to the field of information analysis of sequencing data, and particularly provides a method for detecting and typing the repetition number of a short tandem repeat sequence based on next-generation sequencing. According to the detection method, two flanking sequences can be compared at the same time across a middle repeated region, and mismatching, insertion and deletion of the flanking sequences are considered at the same time; the typing method is an STR typing method for performing dynamic threshold setting by combining repetition number difference based on detection of an effective peak value, and can be applied to detection of repetition numbers of different next-generation sequencing platforms.

The present invention provides a composite amplification system, for analyzing multiple STR loci at the same time, where it amplifies the 20 loci: Amelogenin, vWA, D21S11, D18S51, D5S818, D7S820, D13S317, D16S539, FGA, D2S1338, D19S433, D6S1043, D12S391, D8S1179, D3S1358, CSF1PO, Penta D, Penta E, TH01 and TPOX. Also provided are the methods and kits for analyzing DNA sample at the same time, as well as the uses thereof.