286 results about "Single copy" patented technology

In a computer system including a CPU, a first storage system coupled to the CPU, a second storage system, and a communication link coupling the second storage system to the first storage system, a method and apparatus for asynchronously mirroring, to the second storage system, a plurality of units of data written by the CPU to the first storage system. In one aspect of the invention, the CPU writes units of data to the first storage system in a first order, and the units of data are asynchronously transmitted over the communication link from the first storage system to the second storage system in a second order that is different than the first order. In another aspect, the units of data are committed in the second storage system in an order that is independent of the order in which the units of data are received at the second storage system. In a further aspect of the invention, a packet of information is transmitted over the communication link to the target storage system to specify a commitment order in which the units of data should be committed in the target storage system. In another aspect of the invention, a single copy of each of the units of data is written into the first storage device, without buffering a copy to support asynchronous mirroring. In a further aspect, the storage locations in the first storage system are organized into a plurality of consistency sets, and the units of data are asynchronously transmitted over the communication link so that each consistency set has a representation in the second storage system that is consistent with a valid representation of the consistency set in the first storage system at some point in time.

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

The present provides a microdissection-based method for identifying a genomic feature present within a visible chromosome region. The method includes steps of: (a) micro-dissecting a single copy of a chromosome to obtain a visible chromosome region; (b) amplifying the visible chromosome region to obtain amplified single chromosome DNA; and (c) subjecting the amplified single chromosome DNA to micro-array analysis whereby such analysis identifies at least one genomic feature present within the visible chromosome region. The method is applicable to determining genomic features including, but not limited to, genomic DNA size, gene content, DNA breakpoint, or DNA polymorphism (e.g., single nucleotide polymorphisms).

Nucleic acid (e.g., DNA) hybridization probes are described which comprise a labeled, single copy nucleic acid which hybridizes to a deduced single copy sequence interval in target nucleic acid of known sequence. The probes, which are essentially free of repetitive sequences, can be used in hybridization analyses without adding repetitive sequence-blocking nucleic acids. This allows rapid and accurate detection of chromosomal abnormalities. The probes are preferably designed by first determining the sequence of at least one single copy interval in a target nucleic acid sequence, and developing corresponding hybridization probes which hybridize to at least a part of the deduced single copy sequence. In practice, the sequences of the target and of known genomic repetitive sequence representatives are compared in order to deduce locations of the single copy sequence intervals. The single copy probes can be developed by any variety of methods, such as PCR amplification, restriction or exonuclease digestion of purified genomic fragments, or direct synthesis of DNA sequences. This is followed by labeling of the probes and hybridization to a target sequence.

The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonculease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.

A system and method for single instance backup. In one embodiment, a method may comprise storing a plurality of duplicate messages on a mail server, where each of the messages includes a copy of an attachment, and storing a copy of each of the plurality of duplicate messages and one or more copies of the attachment to a backup medium, where the number of copies of the attachment stored on the backup medium is less than the number of duplicate messages stored on the backup medium. For example, only a single copy of the attachment may be stored on the backup medium. In one embodiment storing a copy of each of the plurality of duplicate messages and one or more copies of the attachment may comprise extracting metadata from each of the messages, storing the metadata in an attachment index, storing the metadata with each message on the backup medium, and storing the metadata with the copies of the attachment on the backup medium.

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The present invention relates, e.g., to a method for amplifying a small number of copies (e.g. a single copy) of a single-stranded circular DNA molecule (e.g. having a size of about 5-6 kb) by an isothermal rolling circle mechanism, using random or partially random primers and a F29-type DNA polymerase. The method, which can also be used for amplifying DNAs by non-rolling types of multiple displacement amplification, comprises incubating the reaction components in a small volume, e.g. about 10 μl or less, such as about 0.6 μl or less. The degree of amplification can be about 109 fold, or higher. A method for cell-free cloning of DNA, using the rolling circle amplification method of the invention, is described.

The Secure Networked Data Shadowing System is connected to a plurality of monitored computer systems via an existing communication medium to store the shadowed data. The data is encrypted by the monitored computer system using a cryptokey, and the data file is processed using a hash function prior to encryption, so the contents of this file are uniquely identified. Thus, the encrypted file is stored in its encrypted form and the hash index is used to identify the encrypted file. A “data de-duplication” process avoids storing multiple copies of the same files by identifying instances of duplication via the hash index. Files that have the same hash index can be reduced to a single copy without any loss of data as long as the file structure information for each instance of the file is maintained.

A data processing system for providing archiving of individual mail content while maintaining a single copy mail store can include a mail application enabled to maintain a single copy mail store, a primary data store configured for high data throughput and acting as a single copy mail store for the mail application, and a secondary data store configured for mass storage and having a lower data throughput than the primary data store. The system further can include at least one archive implementation of an archive interface, the archive interface defining an archive task and a restore task. In one aspect of the embodiment, the system can include each of a content table, a content map table and a restore queue. Furthermore, the system can include a map view of archived content for a specified user, the map view providing a user interface for activating the restore task.

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

A method for detecting single copies of a gene in-situ using brightfield microscopy is used in detection of nucleic acid sequences. Probes are directly or indirectly labeled with alkaline phosphatase with NBT / BCIP used as the chromogen.