286 results about "Single copy" patented technology

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

A centralized interrupt controller with a single copy of APIC logic provides APIC interrupt delivery services for all processing units of a multi-sequencer chip or system. An interrupt sequencer block of the centralized interrupt controller schedules the interrupt services according to a fairness scheme. At least one embodiment of the centralized interrupt controller also includes firewall logic to filter out transmission of selected interrupt messages. Other embodiments are also described and claimed.

In a computer system including a CPU, a first storage system coupled to the CPU, a second storage system, and a communication link coupling the second storage system to the first storage system, a method and apparatus for asynchronously mirroring, to the second storage system, a plurality of units of data written by the CPU to the first storage system. In one aspect of the invention, the CPU writes units of data to the first storage system in a first order, and the units of data are asynchronously transmitted over the communication link from the first storage system to the second storage system in a second order that is different than the first order. In another aspect, the units of data are committed in the second storage system in an order that is independent of the order in which the units of data are received at the second storage system. In a further aspect of the invention, a packet of information is transmitted over the communication link to the target storage system to specify a commitment order in which the units of data should be committed in the target storage system. In another aspect of the invention, a single copy of each of the units of data is written into the first storage device, without buffering a copy to support asynchronous mirroring. In a further aspect, the storage locations in the first storage system are organized into a plurality of consistency sets, and the units of data are asynchronously transmitted over the communication link so that each consistency set has a representation in the second storage system that is consistent with a valid representation of the consistency set in the first storage system at some point in time.

A method and mechanism is disclosed for implementing storage and retrieval of data in a computing system. Data compression is performed on stored data by reducing or eliminating duplicate values in a database block. Duplicated values are eliminated within the set of data that is to be stored within a particular data storage unit. Rather than writing the duplicated data values to the data storage unit, the on-disk data is configured to reference a symbol table a single copy of each duplicated data value. Column reordering may be performed in an embodiment to further improve compression efficiency. The column reordering may be performed to allow efficient removal of trailing NULL values from on-disk storage.

Single copy sequences suitable for use as DNA probes can be defined by computational analysis of genomic sequences. The present invention provides an ab initio method for identification of single copy sequences for use as probes which obviates the need to compare genomic sequences with existing catalogs of repetitive sequences. By dividing a target reference sequence into a series of shorter contiguous sequence windows and comparing these sequences with the reference genome sequence, one can identify single copy sequences in a genome. Probes can then be designed and produced from these single copy intervals.

A method for detecting single copies of a gene in-situ using brightfield microscopy is used in detection of nucleic acid sequences. Probes are directly or indirectly labeled with alkaline phosphatase with NBT / BCIP used as the chromogen.

A method for retrofitting DNA in a single-copy or high-copy vector, such as a fosmid or BAC, whereby an artificial transposon is used to introduce a conditional multi-copy origin of replication (“ori”) into the DNA in said vector. Following random in vitro or in vivo transposition of the ori-containing transposon into DNA in the single-copy or low-copy vector, the resulting insertion clones are introduced into a special host strain that contains a gene which encodes a polypeptide required for replication from the multi-copy ori. However, since the gene for this polypeptide is expressed from a tightly-regulated inducible promoter, the polypeptide is not expressed in the absence of inducer. On addition of inducer to the culture medium, the host cell synthesizes the polypeptide, which in turn activates replication from the multi-copy ori, thereby increasing the amount of clone DNA synthesized by the cell.

The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonculease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.

The present provides a microdissection-based method for identifying a genomic feature present within a visible chromosome region. The method includes steps of: (a) micro-dissecting a single copy of a chromosome to obtain a visible chromosome region; (b) amplifying the visible chromosome region to obtain amplified single chromosome DNA; and (c) subjecting the amplified single chromosome DNA to micro-array analysis whereby such analysis identifies at least one genomic feature present within the visible chromosome region. The method is applicable to determining genomic features including, but not limited to, genomic DNA size, gene content, DNA breakpoint, or DNA polymorphism (e.g., single nucleotide polymorphisms).

Nucleic acid (e.g., DNA) hybridization probes are described which comprise a labeled, single copy nucleic acid which hybridizes to a deduced single copy sequence interval in target nucleic acid of known sequence. The probes, which are essentially free of repetitive sequences, can be used in hybridization analyses without adding repetitive sequence-blocking nucleic acids. This allows rapid and accurate detection of chromosomal abnormalities. The probes are preferably designed by first determining the sequence of at least one single copy interval in a target nucleic acid sequence, and developing corresponding hybridization probes which hybridize to at least a part of the deduced single copy sequence. In practice, the sequences of the target and of known genomic repetitive sequence representatives are compared in order to deduce locations of the single copy sequence intervals. The single copy probes can be developed by any variety of methods, such as PCR amplification, restriction or exonuclease digestion of purified genomic fragments, or direct synthesis of DNA sequences. This is followed by labeling of the probes and hybridization to a target sequence.

The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonculease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.

Various systems and methods are disclosed to share a single copy of a storage object among clones. For example, one method involves creating a first and second clone. The first and second clones share a single copy of a storage object. The first clone is assigned an identifier based on the value of a variable. After creating the first clone the variable is incremented. The second clone is assigned the value of the incremented variable as an identifier.

The Secure Networked Data Shadowing System is connected to a plurality of monitored computer systems via an existing communication medium to store the shadowed data. The data is encrypted by the monitored computer system using a cryptokey, and the data file is processed using a hash function prior to encryption, so the contents of this file are uniquely identified. Thus, the encrypted file is stored in its encrypted form and the hash index is used to identify the encrypted file. A “data de-duplication” process avoids storing multiple copies of the same files by identifying instances of duplication via the hash index. Files that have the same hash index can be reduced to a single copy without any loss of data as long as the file structure information for each instance of the file is maintained.

The subject application is directed to a print proofing system and method for fee-based document output devices. Electronic document data is first received of a selected electronic document for which tangible output is desired. A user is prompted for input of proof output data representing an instruction to generate a single, tangible copy of the electronic document. Proof output instruction data is received representing a desired sample tangible output corresponding to the electronic document. Output is then commenced of the copy corresponding to the received electronic document. Proof acceptability data is received representing the acceptability of the single, tangible copy of the electronic document data. The receipt of proof output instruction data is then disabled upon receipt of proof acceptability data indicating disapproval of the single, tangible copy. Upon receipt of approval of the single copy, fee data is generated associated with the tangible output of the received electronic document.

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses a circulating tumor cell identification kit and a circulating tumor cell identification method. The circulating tumor cell identification kit comprises a detection probe for detecting mRNA of an epithelial cell marking gene and / or mRNA of a mesenchymal cell marking gene, wherein the epithelial cell marking gene is selected from one or more of CDH2, VIMENTIN, FN1 and AKT2. Multiple RNA probes are adopted in the identification method, specific genes of a plurality of circulating tumor cells (CTCs) can be marked at the same time and are divided into genes I (epithelial genes), genes II (epithelial-mesenchymal genes) and genes III (mesenchymal genes), and false positive results caused by lack of part of the specific genes of the CTCs in a process that the CTCs enter peripheral blood for circulation are reduced; the method can be finished within eight hours, and a single copied mRNA hybridization probe is combined with a corresponding fluorescent probe by virtue of a signal amplification system, so that the detection sensitivity of in-situ RNA (Ribonucleic Acid) hybridization is remarkably improved.

The invention relates to a CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9-system-based Saccharomyces cerevisiae genome concurrent multiplex edition vector and application thereof. Cas9 protein expression cassettes and a plurality of sgRNA (small guide ribonucleic acid) scaffold expression cassettes are integrated into the vector to obtain a vector-Cas9-sgRNA scaffold, and a plurality of sgRNA segments designed and synthesized by using ADE1 as a target are integrated into the vector-Cas9-sgRNA scaffold, thereby obtaining the Saccharomyces cerevisiae genome concurrent multiplex edition vector. By adopting a simple substance granule single-copy vector system, one plasmid is utilized to simultaneously express the Cas9 protein and three sgRNA scaffolds, so that three target genes can be edited simultaneously; and thus, the multigene edition operation is very simple. Besides, the target gene edition system only needs one-step component and conversion, and thus, is simple to operate.

A processor method and apparatus that allows for the overlapped execution of multiple iterations of a loop while allowing the compiler to include only a single copy of the loop body in the code while automatically managing which iterations are active. Since the prologue and epilogue are implicitly created and maintained within the hardware in the invention, a significant reduction in code size can be achieved compared to software-only modulo scheduling. Furthermore, loops with iteration counts less than the number of concurrent iterations present in the kernel are also automatically handled. This hardware enhanced scheme achieves the same performance as the fully-specified standard method. Furthermore, the hardware reduces the power requirement as the entire fetch unit can be deactivated for a portion of the loop's execution. The basic design of the invention involves including a plurality of buffers for storing loop instructions, each of which is associated with an instruction decoder and its respective functional unit, in the dispatch stage of a processor. Control logic is used to receive loop setup parameters and to control the selective issue of instructions from the buffers to the functional units.

A data processing system for providing archiving of individual mail content while maintaining a single copy mail store can include a mail application enabled to maintain a single copy mail store, a primary data store configured for high data throughput and acting as a single copy mail store for the mail application, and a secondary data store configured for mass storage and having a lower data throughput than the primary data store. The system further can include at least one archive implementation of an archive interface, the archive interface defining an archive task and a restore task. In one aspect of the embodiment, the system can include each of a content table, a content map table and a restore queue. Furthermore, the system can include a map view of archived content for a specified user, the map view providing a user interface for activating the restore task.

A job joining capability is used in a Multifunction Peripheral Device (MFP) to conduct continuous Raster Image Processing (RIP) across multiple job boundaries. Print jobs which do not have inter-RIP conflicts are printed back-to-back as a continuous single RIP. This is particularly advantageous for single copy, single page, and other small print jobs.

The present invention comprises, without limitation, systems, methods, and compositions for the detection, identification, and quantification, down to the single copy level, of human papillomavirus (HPV) in biological samples, including but not limited to, mammalian body fluids and cervix scrapings, for purposes of detection, treatment and / or management of cancer and dysplasia.

The embodiment of the invention discloses a method, a device and a sever for writing, modifying and restoring data. The scheme provided by the embodiment of the invention starts from three basic operations of writing, modifying and restoring the data on object servers respectively, and ensures the consistency when a plurality of copies of same object data are simultaneously stored on different object servers through a series of methods, thereby greatly reducing the probability of inconsistent data between the copies, fundamentally preventing the appearance of a single copy, and greatly improving the reliability of a distributed file system.

The invention discloses a CRISPR / Cas9-mediated large DNA fragment assembling method. Specifically, the invention provides a nucleic acid construct capable of carrying out constitutive expression of Cas9 in yeast; the nucleic acid construct contains a yeast Tef1 promoter operationally connected with a Cas9 gene, a replication origin from pBR322 and a screening mark thereof, and a replication region from CEN6ARS4 and a screening mark thereof; and the nucleic acid construct is in the form of single-copy replication in the yeast and in the form of high-copy replication in Escherichia coli. The invention also provides a nucleic acid construct capable of carrying out constitutive expression of sgRNA in yeast, a nucleic acid construct used as a receptor vector and a DNA assembling method. According to the invention, two or more to-be-assembled large DNA fragments are successfully assembled in microzyme to form a large plasmid in one shot; so low-efficiency in-vitro digestion recovery, genetic transformation and the like of large DNA fragments are avoided, and the method provided by the invention is more convenient and efficient compared with traditional methods.

The invention relates to a saccharomyces cerevisiae genome editing carrier based on a CRISPR-Cas9 system and application of the carrier. According to the saccharomyces cerevisiae genome editing carrier, a Cas9 protein expression cassette and an sgRNA scaffold expression cassette are integrated in the carrier, and a carrier-Cas9-sgRNA scaffold is obtained; any one of multiple sgRNA fragments which are designed and synthesized by taking ADE1 as a target site is integrated in the carrier-Cas9-sgRNA scaffold, and the saccharomyces cerevisiae genome editing carrier is obtained. According to the saccharomyces cerevisiae genome editing carrier based on the CRISPR-Cas9 system and the application of the carrier, a single plasmid and single copy carrier system is adopted, one plasmid is adopted to express Cas9 protein and sgRNA scaffold simultaneously, construction and conversion of a target gene editing system only need one step, and operation is easy and convenient.

The invention discloses an upland cotton SNP marker and application thereof, and belongs to the field of upland cotton SNP marker development. The upland cotton SNP marker is obtained by developing a single-copy SNP marker of an upland cotton genome via chip hybridization and homologous sequence comparison, and picking out SNP markers in a linkage disequilibrium recession distance via linkage disequilibrium analysis. The SNP marker provided by the invention can be applied to analysis of genetic diversity and group structure, or to germplasm identification of upland cotton, is single-copy, and has no remarkable linkage disequilibrium among markers, so that the efficiency of detection, adopting the SNP marker, during analysis of genetic diversity, group structure or fingerprint identification can be greatly improved, and the work efficiency is higher.

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.