The invention discloses a recombinase-mediated nucleic acid isothermal amplification reaction detecting method. The nucleic acid isothermal amplification reaction detecting method comprises the following step that 1, an amplification reaction system is prepared. The amplification reaction system mainly comprises a, a nucleic acid sample to be detected; b, a pair of oligonucleotides primer 1 and primer 2, wherein the primer 1 and the primer 2 are cross-fertilized with the nucleic acid sample; c, a fluorescent probe, wherein the fluorescent probe is one or multiple of a molecular beacon, a fluorescent resonator, a scorpion probe and a molecular torch, and the concentration of the fluorescent probe ranges from 50-900 nM / L; d, recombinase; e, DNA polymerase; f, single-stranded DNA binding protein; g, amplification reaction buffer. The invention further provides a detection kit based on the nucleic acid isothermal amplification reaction detecting method. According to the nucleic acid isothermal amplification reaction detecting method and the detection kit based on the nucleic acid isothermal amplification reaction detecting method, the complexity of the system is reduced, reagent cost is reduced, sequence synthesis of the probe can be achieved easily, the probe can be designed easily, and actual application and popularization are facilitated.