The invention relates to a
feline coronavirus fluorescence EMA detection primer group, a kit and a detection method. The
feline coronavirus fluorescence EMA detection primer group comprises an upstream primer, a downstream primer and a probe, the kit comprises a reconstitution fluid, a lyophilized
enzyme tube containing primers, a positive
quality control material and a negative
quality control material. The detection method comprises the steps that the kit is used for performing a normal-temperature
nucleic acid amplification technology, through the multiple enzymatic reactions of M-MLV
reverse transcriptase, Rnase inhibitor,
DNA helicase,
single stranded DNA binding protein,
DNA polymerase and the like, to-be-detected
DNA is reversely transcribed to cDNA within 10-30 minutes under the constant temperature of 37-45 DEG C, the cDNA is amplified by millions of times, and rapid
verification of the to-be-detected DNA can be achieved by cooperating with a
fluorescence detection technology.The
feline coronavirus fluorescence EMA detection primer group, the kit and the detection method have the advantages that the operation is simple, the time is short, the requirements for an instrument are low, and the feline
coronavirus fluorescence EMA detection primer group, the kit and the detection method are suitable for the rapid diagnosis for pet infectious diseases.