34 results about "Ustilago esculenta" patented technology

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses an Ustilago esculenta haploid strain UET2 and a use thereof and belongs to the technical field of biology. The Ustilago esculenta haploid strain UET2 has a preservation number of CGMCC No. 11844. Zizania aquatica artificial breeding needs common invasion of two sexual compatible haploid strains. The strain can be used as a zizania aquatica artificial breeding mother strain, through screening, a sexual compatible haploid strain is obtained and artificial zizania aquatica breeding is realized through artificial inoculation. The haploid strain UEMT2 can be used for gene engineering reconstruction and provides an implementation base material for zizania aquatica improvement such as zizania aquatica environmental adaptability and zizania aquatica growth time control in zizania aquatica breeding.

The invention discloses an ustilago esculenta teliospore formation related gene Itd1 and an application thereof and belongs to the technical field of molecular biology. On one hand, the key gene Itd1promoting formation of field zizania latifolia infected with sporidial strain of ustilago esculenta and ustilago esculenta teliospore is provided, on the other hand, the application of the key gene Itd1 to normal pregnant zizania latifolia is provided, and a new direction is provided for zizania latifolia breeding.

The invention discloses a method for artificially breeding normal wild rice shoots, and belongs to the technical field of wild rice shoot cultivation. The method comprises the following steps that Ustilago esculenta haploid strains UET1deltaLtd1 and Ustilago esculenta haploid strain solutions are mixed isovolumetrically for inoculation; 3-5 leaf stage wild rice shoot seedlings are selected as inoculation objects; the mixed bacteria solution is injected to the seedling base through an injector till the bacteria solution overflows from the upper portion of sheaths; the processed inoculation seedlings are trimmed; transition culture is performed, and inoculation is performed after two weeks, and transplanting can be performed for outdoor cultivation; transplanting to outdoor cultivation is performed. The Ustilago esculenta of Ustilago esculenta haploid strains UET1delta ltd1 and Ustilago esculenta haploid strains UET2delta ltd 1 is inoculated to wild rice shoots, and normal wild rice shoots can be successfully produced. The method is easy to implement and effective, and a new direction is provided for wild rice shoot breeding.

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

The invention discloses a pair of ustilago esculenta for successfully achieving artificial breeding of normal zizania caduciflora and application of the pair of ustilago esculenta, and belongs to thefield of biotechnology. The pair of ustilago esculenta comprises an ustilago esculenta haploid strain UET1 delta Itd1 and an ustilago esculenta haploid strain UET2 delta Itd1, wherein the ustilago esculenta haploid strain UET1 delta Itd1 has the preservation number of CGMCC No. 16723; and the ustilago esculenta haploid strain UET2 delta Itd1 has a preservation number of CGMCC No. 16724. Accordingto the invention, the sexual compatible ustilago esculenta haploid strain UET1 delta Itd1 and ustilago esculenta haploid strain UET2 delta Itd1 are obtained by an artificial breeding method; the pairof ustilago esculenta can be inoculated in zizania aquatica to successfully produce normal zizania caduciflora, thereby providing a new idea for the artificial breeding of zizania caduciflora.

The invention discloses an Ustilago esculenta haploid strain UEMT2 and a use thereof and belongs to the technical field of biology. The Ustilago esculenta haploid strain UEMT2 has a preservation number of CGMCC No. 11841. Zizania aquatica artificial breeding needs common invasion of two sexual compatible haploid strains. The strain can be used as a zizania aquatica artificial breeding mother strain, through screening, a sexual compatible haploid strain is obtained and artificial zizania aquatica breeding is realized through artificial inoculation. The haploid strain UEMT2 can be used for gene engineering reconstruction and provides an implementation base material for zizania aquatica improvement such as zizania aquatica environmental adaptability and zizania aquatica growth time control in zizania aquatica breeding.

The invention discloses a method for indoor quick recognition of resistance of wheat to wheat root rot Ustilago esculenta black embryo disease. The method comprises the following steps: firstly, performing surface sterilization on wheat seeds, and performing seedling culture on the sterilized wheat seeds; preparing a wheat root rot boehmeria sorokiniana spore suspension, so as to obtain a spore suspension; shaking up the spore suspension liquid, spraying the spore suspension liquid onto wheat leaves with one leaf and one core for inoculation culture, and recording the percentage of the scab area of the first leaf of the wheat seedling in the total leaf area on the tenth day of inoculation culture, namely the scab area percentage; and calculating the wheat black embryo rate according to theequation, and evaluating the resistance of the wheat to the wheat root rot Umbilical Verticillium dahliae black embryo disease by utilizing the black embryo rate. Compared with an existing field recognition method, the recognition method has the advantages that the recognition time is shortened, the recognition link is simplified, the recognition efficiency is greatly improved, and the accuracy and reliability of the recognition result are improved.

The present invention discloses a kind of Ustilago esculenta strain UEZC1 capable of producing antifungal antibiotic and its use. The Ustilago esculenta strain UEZC1 features that the teleutospore is germinated on potato glucose liquid culture medium to form basidium promycelium with 1-4 basidiospore promycelia, and the basidiospore after falling off further grow to form fusiform short hypha for inserting basidiospore. The present invention can produce antifungal antibiotic, and may be used in developing and producing medical and agricultural antifungal antibiotic.

The invention relates to a haploid strain of Ustilago smut UeTSP and an application thereof, belonging to the field of biotechnology. On the one hand, the present invention provides a new Ustilago smut haploid strain UeTSP, and on the other hand provides the use of the Ustilago smut haploid strain UeTSP in pregnant bamboo. Beneficial effects of the present invention: the smut fungus of the present invention ( Ustilago esculenta ) The haploid strain UeTSP can directly infect Zizania japonica plants and make Zizania zizania gestation without producing teliospores, that is, without the unfavorable phenotype of gray horns, which provides a new idea for zizania zizania artificial pregnancy.

The invention relates to a haploid strain of Ustilago smut UET1 and its application, belonging to the field of biotechnology. The smut fungus ( Ustilago esculenta ) haploid strain UET1, the preservation number is: CGMCC No.11843. Zizania artificial breeding requires the co-infection of two sexually compatible haploid strains. This strain can be used as the mother strain of Zizania artificial breeding. Artificially pregnant watermelon. At the same time, the haploid strain can be genetically engineered to provide basic materials for the subsequent improvement of Zizania varieties, such as improving the environmental adaptability of Zizania, controlling the time of mating and so on.

The invention relates to a haploid strain of Ustilago smut UeMTSP and its application, belonging to the field of biotechnology. On the one hand, the present invention provides a new Ustilago smut haploid strain UeMTSP, and on the other hand provides the use of the Ustilago smut haploid strain UeMTSP in pregnant bamboo. Beneficial effects of the present invention: the smut fungus of the present invention ( Ustilago esculenta ) The haploid strain UeMTSP can directly infect Zizania japonica plants and make Zizania zizania gestation without producing teliospores, that is, without the unfavorable phenotype of gray horns, which provides a new idea for zizania zizania artificial pregnancy.

The invention relates to a pair of wild rice smut fungus which successfully realizes the artificial breeding of Zizania and normal Zizania and its application, belonging to the field of biotechnology. The pair of Ustilago smut includes haploid strain UET1△Itd1 and UET2△Itd1. The Ustilago haploid strain UET1△Itd1 has a preservation number of CGMCC No. .16723, the preservation number of the Ustilago smut haploid strain UET2△Itd1 is CGMCC No.16724. The present invention obtains the sexually compatible Ustilago smut haploid strain UET1△Itd1 and Ustilago smut haploid strain UET2△Itd1 through artificial breeding, and uses the pair of Ustilago to inoculate wild bamboo , can successfully conceive normal Zizania, which provides a new idea for artificial inoculation of Zizania.

The invention discloses an endogenous strong promoter pHSP of smut smut and its expression vector and application, belonging to the technical field of genetic engineering. The present invention includes: a strong endogenous promoter pHSP of Ustilago smut, the nucleotide sequence of which is shown in SEQ ID NO.1; an expression vector containing strong endogenous promoter pHSP of Ustilago smut; Driving the transcription and expression of the eGFP gene, constructing a stable expression system and obtaining the application of engineering smut smut has improved eGFP expression intensity and fluorescence stability. The present invention combines endogenous pHSP promoter and strong terminator nosT of Ustilago smut with eGFP The genes are connected to construct an effective plasmid vector pUe-cbx-Hsp, which improves eGFP The expression intensity of the start eGFP Compared with otef and TFIID2, the gene transcription level was enhanced by more than 9 times and 11 times respectively.

The invention relates to the field of biological breeding, in particular to an Agrobacterium-mediated Ustilago esculenta transformant strain and a preparation method and application thereof. Agrobacterium mediates the transformant strain of Ustilago esculenta, and the Agrobacterium infection receptor of the transformant strain is Ustilago esculenta. A method for preparing the above-mentioned Agrobacterium-mediated transformation of Zizania smut transformant strain, the steps are as follows: 1) Preparation of fungal expression vector; 2) Preparation of Agrobacterium competent; 3) Electrotransformation of Agrobacterium; 4) Agrobacterium-mediated transformation Guided transformation to obtain the transformant. The present invention does not need to prepare complex protoplasts, the source of the receptor is simple, and the spores and hyphae of the fungus can be directly used for transformation; the transformation efficiency is high; in addition, the single-copy ratio of the obtained transformants is relatively large, which is beneficial to obtain marker genes .

The invention discloses a culture medium capable of maintaining in-vitro growth of hyphae of ustilago esculenta, and belongs to the technical field of culture media. Every liter of the culture medium comprises the following components: 0.85 to 1.15 grams of K2HPO4, 0.45 to 0.55 gram of MgSO4.7H2O, 0.008 to 0.012 gram of FeSO4.7H2O, 0.45 to 0.55 gram of KCl, 2 to 3 grams of yeast powder, 4 to 6 grams of peptones, 3 to 4 grams of sucrose, 4.5 to 5.9 grams of histidine and 10 to 15 grams of agar. The culture medium capable of maintaining maintaining in-vitro growth of the hyphae of the ustilago esculenta is reasonable in compatibility; optimal carbon and nitrogen sources are added into a basic culture medium, so that in-vitro growth of the hyphae of the ustilago esculenta can be maintained by the culture medium.

The invention discloses an Ustilago esculenta typing identification method and application thereof, and belongs to the technical field of molecular biology. The invention provides a target for typing identification of Ustilago esculenta. The target is derived from T-type Ustilago esculenta, and the nucleotide sequence of the target is as shown in SEQ ID No. 1. According to the Ustilago esculenta typing identification method provided by the invention, T-type Ustilago esculenta in a complex sample can be quantitatively detected, the detection limit is as low as 0.0088 ng / [mu] L (22.36 copies / [mu] L), and meanwhile, the quantitative detection of the T-type Ustilago esculenta in different tissues in different stages of plants is realized.

The invention discloses an ustilago esculenta endogenous promoter pEF as well as an expression vector and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the ustilago esculenta endogenous promoter pEF is shown as SEQ ID NO.1. The invention discloses the expression vector containing the ustilago esculenta endogenous promoter pEF, an application of the ustilago esculenta endogenous promoter pEF to driving transcription and expression of an eGFP gene, constructing a stable expression system and obtaining engineering ustilago esculenta, and an application of the ustilago esculenta endogenous promoter pEF to improving expression intensity and fluorescence stability of eGFP. The ustilago esculenta endogenous pEF promoter and a strong terminator nosT are connected with the eGFP gene, an effective plasmid vector pUe-cbx-EF is constructed, more choices are provided for construction of an ustilago esculenta genetic transformation vector, and a foundation is laid for functional gene research of the ustilago esculenta.

The invention relates to a haploid strain UEMT2 of smut smut and its application, belonging to the field of biotechnology. In the present invention, smut fungus ( Ustilago esculenta ) haploid strain UEMT2, the preservation number is: CGMCC No.11841. Zizania artificial breeding requires the co-infection of two sexually compatible haploid strains. This strain can be used as the mother strain of Zizania artificial breeding and after screening to obtain its sexually compatible haploid strains, it can be achieved by artificial inoculation. Artificially pregnant watermelon. At the same time, the haploid strain can be genetically engineered to provide basic materials for the subsequent improvement of Zizania varieties, such as improving the environmental adaptability of Zizania, controlling the time of mating and so on.