36 results about "Smut fungus" patented technology

The invention relates to the field of biology breeding and in particular relates to an agrobacterium-mediated ustilago esculenta (Ustilago esculenta) transformant strain as well as a preparation method and application thereof. According to the agrobacterium-mediated ustilago esculenta transformant strain, an agrobacterium infected receptor of the transformant strain refers to ustilago esculenta (Ustilago esculenta). The method for preparing the agrobacterium-mediated ustilago esculenta transformant strain comprises the following steps: (1) preparing a fungal expression vector; (2) preparing agrobacterium competence; (3) performing electrotransformation on the agrobacterium; and (4) performing agrobacterium-mediated transformation, thereby obtaining the transformant. A complex protoplast is not needed, the receptor source is simple, and spores and hypha of the fungi can be directly used for transformation; and the transformation efficiency is high. In addition, the obtained transformant is large in single-copy ratio, and marker genes are conveniently obtained.

The invention discloses an Ustilago esculenta haploid strain UET1 and a use thereof and belongs to the technical field of biology. The Ustilago esculenta haploid strain UET1 has a preservation number of CGMCC No. 11843. Zizania aquatica artificial breeding needs common invasion of two sexual compatible haploid strains. The strain can be used as a zizania aquatica artificial breeding mother strain, through screening, a sexual compatible haploid strain is obtained and artificial zizania aquatica breeding is realized through artificial inoculation. The haploid strain UEMT2 can be used for gene engineering reconstruction and provides an implementation base material for zizania aquatica improvement such as zizania aquatica environmental adaptability and zizania aquatica growth time control in zizania aquatica breeding.

The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.

This invention encompasses the identification and isolation of genes that confer disease control properties in plants, as well as plants comprising such genes. These genes are derived from the following sources: Nicotiana benthamiana, Oryzae sativa (var. Indica IR7), Papaver rhoeas, Saccharomyces cerevisiae and Trichoderma harzianum (Rifai 1295-22). The control conferred is against the one or more of the following phytopathogens: Aspergillus flavus, Cercospora zeae-maydis, Fusarium monilforme, Fusarium graminearum, Helminthosporium maydis, Phoma lingam, Phomopsis helianthi, Phytopthera infestans, Pyricularia oryzae, Pythium ultimum, Rhizoctonia solani, Sclerotinia sclerotiorum, Ustilago maydis, and Verticillium dahliae. Further, this invention encompasses other homologous and heterologous sequences with a high degree of functional similarity.

The invention discloses a method and a special kit for detecting Ustilago maydis. The invention also provides a set of ring-mediated isothermal amplification primers for detecting the Ustilago maydis.The set of primers comprise a primer Pep-F3-2, a primer Pep-B3-2, a primer Pep-FIP(F1c+F2)-2, a primer Pep-BIP(B1c+B2)-2, a primer Pep-LoopF-2 and a primer Pep-LoopB-2. The method can be used for field sample detection, and provide a basis for the prevention and control of common diseases of corn and the resistance breeding. An LAMP detection system developed in the invention can provide effective guidance for the comprehensive prevention and control of the Ustilago maydis, avoids the further occurrence of diseases, and is also an important auxiliary means for the research work of plant-pathogen molecular interaction and disease resistance breeding.

The invention discloses an ustilago esculenta teliospore formation related gene Itd1 and an application thereof and belongs to the technical field of molecular biology. On one hand, the key gene Itd1promoting formation of field zizania latifolia infected with sporidial strain of ustilago esculenta and ustilago esculenta teliospore is provided, on the other hand, the application of the key gene Itd1 to normal pregnant zizania latifolia is provided, and a new direction is provided for zizania latifolia breeding.

The invention discloses a method for improving yield of fruiting bodies of cordyceps takaomontana. The method comprises the steps of activating an ustilago esculenta GZUIFR-DX091 bacterial strain by applying a PDA culture medium, picking three loops for the activated bacterial strain by applying an inoculating loop to access into the ustilago esculenta culture medium, centrifuging culture liquor for 5 minutes at 5000r / min after culturing for 5 days at 28 DEG C at 160r / min, and getting liquid supernatant to obtain fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain; infecting silkworms with cordyceps takaomontana, and starting producing fruiting bodies for later use when the infected silkworms are hardened; pre-sterilizing a culture container, and tiling single layer or double layers of sterilized water-absorbing paper at the bottom of the container; slowly adding the prepared fermentation liquor of the ustilago esculenta GZUIFR-DX091 bacterial strain onto the water-absorbing paper at the bottom of the container, placing the infected silkworms into a container which is added with the cane shoot fermentation liquor, and carrying out culture under light for 12 hours at 20-23 DEG C, wherein the culture temperature can be lowered by 5 DEG C for culturing at a dark culture stage. In the culture process, if the added fermentation liquor is dried, the fermentation liquor is needed to be timely complemented; the fruiting bodies of cordyceps takaomontana can be harvested after culturing for 3-4 weeks. The method disclosed by the invention can greatly improve the yield of the fruiting bodies of cordyceps takaomontana.

The invention discloses a sterilizing composition. The effective components are Sedaxane and captan, the mass ratio of the both is 50:1-1:50. The composition has obvious synergism and is effective totreat diseases caused by rhizoctonia solani, puccinia horiana, pyrenophora graminea, ustilago esculenta, Tilletia, Erysiphe, pyrenophora teres, Septoria musiva, and Ramularia; the composition has special effect to seed-borne disease. The composition can be applied to treat seeds and spray stems and leaves, the effect duration lasts for 4-6 weeks; the composition can be applied to the disease prevention and treatment of cereal type crops; particularly, it is applied to the seed treatment for preventing wheat ustilago esculenta, wheat tilletia, wheat basal rot, sphacelotheca reiliana, corn stalkrot, corn gall smut and rice bakanae disease.

The invention discloses a novel method for rapidly identifying the resistance level of sugarcane smut, which comprises the following steps: selecting a mature plant which grows vigorously, cutting off sugarcane tips, and binding the tail ends of stems with a plastic film; the smut fungus teliospore suspension with the concentration of 5 * 10 < 6 > spores / m L is prepared from smut fungus teliospore with the germination rate of 90% or above. A medical injector is adopted to inject about 25 microliters of smut fungus teliospore (the concentration is 5 * 10 < 6 > spores / mL) suspension into each sugarcane bud of a plant, and about 90 buds are inoculated to each sugarcane material. After inoculation, the sugarcane material is still planted in a greenhouse and normally managed, the morbidity condition of sugarcane smut is investigated regularly, the total morbidity plant number is counted, and the final morbidity of each treatment is calculated. Positive control is set in the test, and resistance evaluation is carried out by meeting sugarcane smut resistance grading standards.

The invention belongs to the field of biopesticides, and discloses a sugarcane endophytic bacillus subtilis CGB12 strain and application thereof. The bacillus subtilis CGB12 strain is preserved in the Guangdong Microbial Culture Collection Center on March 15, 2021, the preservation number is GDMCC No: 61561, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The bacillus subtilis CGB12 strain has a strong inhibition effect on both sugarcane smut and pyricularia grisea, has a good biological control effect, is small in environmental pollution, few in side effect and good in environmental protection effect when being used for biological control, and has a good biological application prospect.

The invention discloses a Ustilago maydis composition. The Ustilago maydis composition comprises a haploid strain UM01 and a haploid strain UM02, wherein the preservation number of the UM01 is CGMCC No. 15263, and the preservation number of the UM02 is CGMCC No. 15387. The ustilago maydis composition is highly pathogenic, high in vaccination success rate, accurate and reliable in identification results of resistance to ustilago maydis, and good in repeatability and consistency; high yield of corn mushroom is achieved when the ustilago maydis composition is applied to the production of the cornmushrooms, the corn mushrooms are wrapped in bracts, and convenience in harvesting and transporting is achieved.

The invention discloses a Ustilago-maydis haploid strain UM02. The collection number of the Ustilago-maydis haploid strain UM02 is CGMCC No.15387. The Ustilago-maydis haploid strain UM02 is combined with a haploid strain matched with the Ustilago-maydis haploid strain UM02 in mating type, then the pathogenicity is high, the success rate of inoculation is high, an identification result of the ustilago maydis resistance of maize varieties is accurate and reliable, and the consistency is good. When the Ustilago-maydis haploid strain UM02 is used for maize mushroom production, the yield of maize mushrooms is high, husk leaves are wrapped with the Ustilago-maydis haploid strain UM02, and harvesting and transportation are convenient.

The invention belongs to the field of biotechnology, and specifically discloses a sugarcane smut SCoT-PCR (polymerase chain reaction) specific primer screening and an application. The primer sequence is shown as SEQ ID NO:1 to 10. Ten primers are used for amplifying ten sugarcane smut matching strains from different areas and hosts, totally, 86 bands are amplified, averagely, each primer amplifies 8.6 bands, the polymorphic rate is 59.3%. The screened specific primer has relatively high polymorphic detection efficiency, and can be used for genetic diversity analysis on sugarcane smut; and theoretical direction for selection of disease-resistant varieties of sugarcane is provided.

The invention discloses a method for breeding zizania latifolia plant seedlings. The method comprises the following steps: extracting DNA (Deoxyribose Nucleic Acid) of a to-be-detected sample containing Ustilago esculenta as an amplification template; a fluorescent quantitative PCR (Polymerase Chain Reaction) method is used for detection, and a primer pair used for detection comprises a forward primer with a nucleotide sequence as shown in SEQ ID No.1 and a reverse primer with a nucleotide sequence as shown in SEQ ID No.2; the nucleotide sequences of the probes used for detecting the MT type Ustilago esculenta and the T type Ustilago esculenta are as shown in SEQ ID No.3 and SEQ ID No.4; predicting the phenotype of the zizania latifolia plant in the mature period according to the ratio of the content of the T-type zizania latifolia to the content of the MT-type zizania latifolia, and breeding zizania latifolia plant seedlings. By analyzing the content of T-type and MT-type zizania esculenta in stem node or stem tip tissues of zizania aquatica plants, whether grey zizania aquatica or male zizania aquatica can be formed in the future can be effectively predicted, so that the zizania aquatica seedling raising process is optimized, and economic losses are reduced.

The invention belongs to the technical field of biology, and particularly relates to a detection primer of Thecaphana schwarzmania, a detection method and application thereof. According to the invention, a specific primer is designed according to the composition difference of basic groups in ITS regions of pathogenic bacteria Thecaphana schwarzmania of the rhubarb smut disease and ustilago fungi;the invention also provides the detection primer for the Thecaphana schwarzmania, the detection method thereof and an application of Thecaphana schwarzmania. The method has the characteristics of rapidness, accuracy and sensitivity, and the convenience, timeliness and accuracy of detecting the disease caused by Thecaphana schwarzmania are effectively improved.

The invention relates to the technical field of microbiology, in particular to ustilago sp. HFJ311 and an application thereof to promotion of plant growth and improvement of tolerance of plants to various stresses. The ustilago sp. HFJ311 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the ustilago sp. HFJ311 is CGMCC No. 18150. The ustilago sp. can promote the growth of overground parts and underground parts of plants and enhance tolerance of the plants to salt, drought and stress of various metal ions. The ustilago sp. can reduce the damage of cadmium ion stress to the plants by promoting the chlorophyll content of plant seedlings, improving in-vivo antioxidant enzyme activity, reducing cadmium ion accumulation and the like. The ustilago sp. HFJ311 can be used for preparing a preparation for promoting plant growth and a soil conditioner, and has a wide application prospect.

The invention discloses CRISPR / Cas9 plasmid as well as a construction method and a use method of the CRISPR / Cas9 plasmid and belongs to the technical field of biotechnology. A nucleotide sequence of the CRISPR / Cas9 is shown in SEQ ID No.3. The CRISPR / Cas9 plasmid for artificial breeding of zizania latifolia is constructed with a CRISPR / Cas9 technology and converts ustilago esculenta, a gene knockout strain can be obtained, and the CRISPR / Cas9 plasmid can be more conveniently and efficiently applied to artificial breeding of the zizania latifolia.

The invention discloses a construction and insertion site analysis method of an Ustilago esculenta T-DNA mutant library, and belongs to the technical field of gene engineering. The method comprises the following steps: by taking a constructed Ustilago esculenta self-fusion strain TSP as a starting strain and a plasmid containing a geneticin resistance gene as a carrier, constructing a Ustilago esculenta T-DNA mutant library through ATMT, carrying out genome re-sequencing on the constructed Ustilago esculenta T-DNA, and analyzing a T-DNA insertion site of the Ustilago esculenta T-DNA. The method lays a foundation for subsequent regulation and control mechanism research of Ustilago esculenta two-form conversion.

The invention discloses an Ustilago esculenta typing identification method and application thereof, and belongs to the technical field of molecular biology. The invention provides a target for typing identification of Ustilago esculenta. The target is derived from T-type Ustilago esculenta, and the nucleotide sequence of the target is as shown in SEQ ID No. 1. According to the Ustilago esculenta typing identification method provided by the invention, T-type Ustilago esculenta in a complex sample can be quantitatively detected, the detection limit is as low as 0.0088 ng / [mu] L (22.36 copies / [mu] L), and meanwhile, the quantitative detection of the T-type Ustilago esculenta in different tissues in different stages of plants is realized.

The invention discloses an ustilago esculenta endogenous promoter pEF as well as an expression vector and application thereof, and belongs to the technical field of gene engineering. The nucleotide sequence of the ustilago esculenta endogenous promoter pEF is shown as SEQ ID NO.1. The invention discloses the expression vector containing the ustilago esculenta endogenous promoter pEF, an application of the ustilago esculenta endogenous promoter pEF to driving transcription and expression of an eGFP gene, constructing a stable expression system and obtaining engineering ustilago esculenta, and an application of the ustilago esculenta endogenous promoter pEF to improving expression intensity and fluorescence stability of eGFP. The ustilago esculenta endogenous pEF promoter and a strong terminator nosT are connected with the eGFP gene, an effective plasmid vector pUe-cbx-EF is constructed, more choices are provided for construction of an ustilago esculenta genetic transformation vector, and a foundation is laid for functional gene research of the ustilago esculenta.