A strain of bacillus and its fermentation product and application

A fermentation product, Bacillus technology, applied in the field of microorganisms to achieve the effects of short production cycle, stable activity, and improved rational structure

Active Publication Date: 2021-04-20
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the 24-membered macrolide produced by the bacillus of the present invention has not been reported at home and abroad, and the application of this compound to the control of sugarcane smut has not been reported.

Method used

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  • A strain of bacillus and its fermentation product and application
  • A strain of bacillus and its fermentation product and application
  • A strain of bacillus and its fermentation product and application

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0033] The present embodiment provides a strain of bacillus (Bacillus sp) IMDGX0108

[0034] The strain has been preserved, and the preservation information is as follows:

[0035] Deposit number: CCTCC NO: M 2018067; Deposit location: China, Wuhan, Wuhan University, China Collection Center for Type Cultures; Deposit date: January 30, 2018.

[0036] The strain is isolated and purified from the surface of marine organisms in the Beibu Gulf. The specific process of isolation and purification is as follows:

[0037] (1) Rinse the surface of marine organisms collected from the Beibu Gulf with sterile water three times, cut out 1cm×1cm small pieces, add 1mL of sterile water to grind, and store the grinding solution in a sterile centrifuge tube until use;

[0038] (2) Gradiently dilute the grinding solution to 10 -1 、10 -2 and 10 -3 . Spread the diluted solution on 8 types of isolation media including M1, M5, M7, M9, M10, AGG, Isp3, and Isp7, and culture at a constant temperatu...

Embodiment 2

[0042] This embodiment provides a preparation method of a Bacillus sp IMDGX0108 fermentation product, comprising the following steps:

[0043] (1) Activate the bacillus isolated from marine organisms, and when the bacteria grow to the logarithmic phase, vibrate in Isp2 medium at 28°C and 180r / min, and ferment for 7 days to obtain a fermentation broth ; Among them, the preparation method of the improved Isp2 medium is as follows: firstly weigh 2 g / L of glucose, 2 g / L of yeast extract, and 2 g / L of malt extract powder, dissolve them in distilled water, and autoclave at 121 ° C for 20 min;

[0044] (2) crushing the fermentation culture liquid with a cell disruptor, performing centrifugal filtration and separation through a polyamide microporous filter membrane to obtain a fermentation filtrate to obtain a fermentation liquid;

[0045] (3) The fermented liquid obtained in step (2) was extracted four times with equal volume of ethyl acetate, concentrated by a rotary evaporator to obt...

Embodiment 3

[0047] This example provides the application of a fermentation product of Bacillus sp IMDGX0108.

[0048] The fermentation product is mainly used in the inhibition of sugarcane smut.

[0049] The following is an analysis of the activity of the fermentation product against the sugarcane smut:

[0050] (1) Collection and preservation of test bacteria

[0051] The teliospores of sugarcane smut smut were collected from diseased sugarcane plants, and the teliospores on fresh smut whips were lightly tapped, packed in sealed bags, dried at 40°C and stored in a refrigerator at 4°C for later use.

[0052] (2) Preparation of sugarcane whip smut basidiospores and mycelium

[0053] (1) Mix a small amount of teliospores with sterile water, dilute and apply multiple concentrations (10 -3 The best, the best concentration is selected based on the single distribution of colonies after coating, and the degree of sparseness is moderate);

[0054] (2) After it germinates, pick a single colony...

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Abstract

The invention provides a strain of Bacillus sp. and its fermentation product and application, and relates to the field of microbiological technology. The bacterium is named Bacillus sp. IMDGX0108, and the preservation number is CCTCC NO: M 2018067; the preservation location is: China, Wuhan, Wuhan University , China Typical Culture Collection Center, the preservation time is January 30, 2018, the fermentation product of this strain is rich in 24-membered macrolide, and its fermentation product has a strong inhibitory effect on sugarcane smut , the fermentation product can be applied to inhibit Ustilago cane whip and be used to prepare biological pesticides capable of inhibiting Ustilago cane whip, and has a good prospect in the development and research of biopesticides.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms, in particular to a strain of bacillus and its fermentation product and application. 【Background technique】 [0002] Macrolactions are a series of 24-membered macrolide compounds, mainly isolated from Bacillus sp. and Actinomadura sp., many of which come from the ocean. So far, there are about 30 macrolactins that have been discovered. As a class of novel antibiotics, 24-membered macrolides have attracted much attention for their application prospects. [0003] Sugarcane is an important sugar crop in the world and also the main sugar crop in my country. It is mainly distributed in Guangdong, Guangxi, Yunnan, Fujian, Hainan and Taiwan. In addition, sugarcane is also a potential renewable energy crop. China is one of the countries where sugarcane is relatively old. As early as the 4th century BC, it was distributed from the Qinling Mountains to Hainan Island in my country. It is an importa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 高程海易湘茜江蕾米顺利杜瑜欣
Owner GUANGXI UNIV OF CHINESE MEDICINE
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