Sugarcane endophytic bacillus subtilis and application thereof

A technology of Bacillus subtilis and Cane stigma, applied in the application, bacteria, biocide and other directions, can solve the problem of not being able to prevent and control at the same time, achieve the elimination of pathogenic risks, low requirements for culture conditions, and easy development and application effect of value

Active Publication Date: 2021-11-05
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But these two strains cannot control sugarca

Method used

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  • Sugarcane endophytic bacillus subtilis and application thereof
  • Sugarcane endophytic bacillus subtilis and application thereof
  • Sugarcane endophytic bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation, purification, identification and preservation of endophytic Bacillus subtilis CGB12 strain in sugarcane leaves

[0045] 1. Preparation of LB medium

[0046] Weigh 10g of tryptone (Tryptone, Oxoid LTD LP0042, England), 5g of yeast extract (Yeasextract, Oxoid LTD LP0021, England), 10g of sodium chloride, add 1000mL of water and stir evenly, add 15g of agar, fully heat and dissolve, and then pack , sterilized at 121°C for 20 minutes and stored for later use.

[0047] 2. Isolation and purification of endophytic Bacillus subtilis from sugarcane leaves

[0048] (1) Leaf collection: The sugarcane leaves used for separation were collected from the sugarcane field of researcher Shen Wankuan of the College of Agriculture, South China Agricultural University, and were roughly divided into new leaves, mature leaves, and old leaves according to the spatial position of the leaves (upper, middle, and lower) Three groups.

[0049] (2) Leaf disinfection: wash 3 t...

Embodiment 2

[0059] Example 2 Determination of the activity of Bacillus subtilis CGB12 strain against sugarcane whip smut and rice blast fungus by confrontation culture method

[0060] 1. Preparation of culture medium

[0061] (1) Preparation of LB liquid medium: except that agar is not added, other formulas are the same as those of LB solid medium in Example 1, and the weighed formula medicament is fully dissolved and subpackaged in conical flasks (100mL culture fluid in each bottle) ), stoppered, bandaged, sterilized at 121°C for 20 minutes, cooled and stored for later use.

[0062] (2) Preparation of PDA medium: PDA broth (purchased from Dingguo), see its instructions for usage.

[0063] (3) Preparation of CM medium: calcium nitrate (10g / 100mL) 10mL, salt solution (2g potassium dihydrogen phosphate, 2.5g magnesium sulfate heptahydrate, 1.5g sodium chloride dissolved in 100mL water) 10mL, yeast extract powder 1.0 g, casein hydrolyzate 0.5g, acid casein hydrolyzate 0.5g, glucose 10g, wa...

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Abstract

The invention belongs to the field of biopesticides, and discloses a sugarcane endophytic bacillus subtilis CGB12 strain and application thereof. The bacillus subtilis CGB12 strain is preserved in the Guangdong Microbial Culture Collection Center on March 15, 2021, the preservation number is GDMCC No: 61561, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The bacillus subtilis CGB12 strain has a strong inhibition effect on both sugarcane smut and pyricularia grisea, has a good biological control effect, is small in environmental pollution, few in side effect and good in environmental protection effect when being used for biological control, and has a good biological application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological control. More specifically, it relates to a sugarcane endophytic Bacillus subtilis and its application. Background technique [0002] Plant disease refers to the phenomenon that plants undergo a series of morphological, physiological and biochemical pathological changes under the influence of biological or abiotic factors, hindering the normal growth and development process, thereby affecting human economic benefits. Plant diseases are antagonistic symbiosis between host plants and pathogens, and their occurrence and prevalence are the result of the interaction between host plants and pathogens. There are many causes of plant diseases, and diseases caused by plant pathogenic fungi account for about 70% to 80% of plant diseases, for example: sugarcane whip smut can be caused by sugarcane whip smut, and rice blast caused by rice blast fungus It is a major disease in rice production and greatly ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/125
CPCC12N1/20A01N63/22
Inventor 邓懿祯毕新萍崔国兵尹凯朱碌媛杨子仪唐金阳
Owner SOUTH CHINA AGRI UNIV
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