88results about How to "The test results are credible" patented technology

The invention relates to amplification composite for detecting the microdeletion of Y-chromosome and a detection kit, belonging to the field of biotechnical detection. The amplification composite for detecting the microdeletion of Y-chromosome can be amplified to as many as 30 sites related to the microdeletion detection of the Y-chromosome through one reaction. The detection kit detects the microdeletion of the Y-chromosome through the quantitative fluorescent PCR (Polymerase Chain Reaction) method by using the amplification composite. The microdeletion abnormality of the Y-chromosome is determined according to the existence of an amplification product and the quantity of the amplification product. A large quantity of sites enables the detection result to be more convincible and can provide much more and more detailed information for determining the deletion type, and the quantitative detection of partial deletion and repetition can be realized. The detection kit is easier and more convenient to operate, only one PCR amplification and one sequencer detection reaction are needed to complete the detection of one sample, the whole process needs 4-5 h, and the operation intensity and the detection time are greatly reduced.

The invention relates to a test method and device of metal ceiling system intensity, which is especially applied under static pressure load and wind pressure dynamic load. The test device is composed of a sample car, a working system and an operation controlling system. Realize test and characterization of metal ceiling system intensity by simulating practical environment of its enlistment. This method can reflect practical environment of metal ceiling really, which guarantees more scientific and perfect test method and more credible test result. It will be extended as a professional testing criterion.

The invention discloses a centering measurement apparatus for measuring the center distance of two intersecting holes in an intersecting hole system. The centering measurement apparatus comprises a two-hole positioning and centering device including an elastic centering expanding sleeve, a centering pull bar, a centering pressure bar, an eccentric wheel compacting mechanism and a positioning pin, a variable-pitch cam mechanism including a handle, a servo-actuated eccentric cam, a bearing positioning circular section and a cooperative column, and an auxiliary centering mechanism, wherein the two-hole positioning and centering device is inserted in a first hole system of a part to be measured, the eccentric wheel compacting mechanism is rotated to move the centering pull bar and centering pressure bar and open the elastic centering expanding sleeve to center the first hole system, and the variable-pitch cam mechanism and the auxiliary centering mechanism are inserted in two sides of a second hole system of the part to be measured. The handle is rotated to drive the cam to rotate, so that the rim of the cam is tangent to the excircle of the elastic centering expanding sleeve, and angle scales corresponding to different center distances of the first hole system and the second hole system are radially marked on the end face of the servo-actuated eccentric cam. The actual center distance of the intersecting holes of the part to be measured can be rapidly obtained by observing the angle graduation lines, and rapid and accurate measurement is realized.

The invention discloses a multi-system integrated intelligent substation relay protection dynamic regular inspection method. The method comprises the steps: setting an automatic inspection period, anautomatic regular inspection period and an early warning period; initializing regular inspection counting time of each inspection item as initial timing time; and carrying out automatic inspection oneach inspection item in the automatic inspection period to obtain corresponding automatic inspection results; and carrying out result confirmation according to the automatic inspection results and resetting regular inspection counting time. According to the invention, in the automatic regular inspection period and before the early warning period, when inspection items all act correctly, and automatic inspection results are normal, the relay protection device can perform regular inspection completely without stopping, and the regular inspection period is prolonged; if the automatic inspection results of the inspection items are no result all the time, the relay protection device enters the early warning period and needs to be stopped for regular inspection, and only the inspection items which do not act can be inspected according to the inspection items, so that the number of the regular inspection items can be reduced to the minimum, the power failure time is shortened, and the regularinspection workload is reduced.

The invention relates to a kind of enzyme colorimetric method and a method of the coupling method measuring the density of citric acid and a diagnosis reagent box applying the citrate synthetase and coupling malic dehydrogenase enzymatic reaction continues monitoring method / ratio colorimetric method. The reaction of citrate synthetase enzymolysis citric acid generates oxaloacetic acid and then through the effect of coupling malic dehydrogenase at last oxidizes the reduced coenzyme (there is a absorption peak at the site of 340nm) becoming the coenzyme (there is a absorption peak at the site of 340nm) so we can assay the degree / speed of the fall-way absorbance at the place of 340nm. It can measure and calculate the density of citric acid by measuring the degree / speed of the fall-way absorbance at the place of 340nm. The method has high specificity and it is not polluted by the endogenous and exogenous object and the test result is precise and accurate. The invention can get the array result by the ultraviolet / visible light analytic instrument so it is convenience to extend and apply.

The invention discloses a coal face physical similar simulation support having a setting load. The coal face physical similar simulation support comprises a support frame, a support column, a force measuring sensing system and a display meter; the support frame comprises a pedestal, a connecting rod, a shield plate and a top guard plate; the force measuring sensing system is fixed to the pedestal in the support frame; the force measuring sensing system is electrically connected with the display meter via a signal transmission line; and the support column is a length-adjustable telescopic column, a lower end of the support column is fixed to an upper plate surface of the force measuring sensing system, and an upper end of the support column is fixed to a lower surface of the top guard plate of the support frame. According to the coal face physical similar simulation support having the setting load, test precision can be improved, the engineering field can be simulated truly, and result reliability can be achieved.

The invention discloses primers, a probe and a kit for detecting the BRCA1 gene expression. The nucleotide sequences of upstream and downstream primers of BRCA1 gene are respectively represented by the SEQ ID No. 1-2. The nucleotide sequence of Taqman fluorescence probe of BRCA1 gene is represented by the SEQ ID No.3. The invention also discloses a detection kit composed of the primers and probe mentioned above, and an application method of the kit. The provided kit can detect samples in different forms such as fresh frozen tissues, paraffin embedded samples, and the like, and has the advantages of high sensitivity, high specificity, and wide application range. The kit comprises premix, a sample can be added directly, a sample does not need to be mixed with enzyme, the operation is convenient and simple, the judgment method becomes more scientific and reasonable, the error generated during the experiment process is reduced, the precision is high, furthermore, the detection speed of the detection kit is quick, and the detection result can be obtained within two hours.

The invention provides a detection method for an upstream pressure of a Venturi tube. The method comprises the following steps: acquiring the upstream pressure of the Venturi tube and a pressure behind a throttle valve and acquiring a pressure difference value of the upstream pressure of the Venturi tube and the pressure behind the throttle valve when an engine is in a stable working condition andan EGR valve is closed; and when the pressure difference value is smaller than a current judgment threshold value, determining that the detection result of the upstream pressure of the Venturi tube is reliable. According to the detection method provided by the invention, the engine is not required to be in an idle state, the upstream pressure of the Venturi tube is also detected in a process thatthe engine operates, and the effectiveness and the accuracy of the detection result are guaranteed, so that the control accuracy of the EGR is further improved. The invention also provides a detection system for the upstream pressure of the Venturi tube.

The invention discloses a gas sampling enrichment device based on nano-fibers, comprising a gas sampling device (1) and a gas enrichment device (2) and characterized in that the gas enrichment device (2) is in control communication with the gas sampling device (1), and the nano-fibers (21) in which gas components are enriched are arranged in the gas enrichment device (2). The device can accurately fix the collection quantity of gas to enable a detection result to be more reliable, can reduce the collection quantity of the gas, in particular gas with less content, can save the time, the labor and the cost and does not need any power system.

The invention discloses a surface roughness testing method and a tester. The testing method comprises the steps of placing a friction assembly on a tested concrete surface so that the friction assembly rotates or slides on the tested concrete surface, measuring physical quantities such as rotating speed, torque, speed and resistance of the friction assembly in movement, calculating the characteristic quantity of the surface roughness or estimating the surface roughness of the tested concrete according to the measured physical quantities and a corresponding calculating formula. The tester for realizing the method comprises a body, a supporting seat and the friction assembly, wherein the friction area of the friction assembly is annular or rectangular. The testing method and the tester of the invention are used for testing and evaluating the concrete surface roughness and have advantages of clear principle, trustable result, wide application range and convenient operation.

The invention discloses a nucleic acid, kit and method for rapid detection of C3435T polymorphism of an ABCB1 gene. The nucleic acid comprises detection primers and a detection probe, wherein the detection primers comprise a wild downstream primer, a mutant downstream primer and a common upstream primer; a nucleotide sequence of the wild downstream primer is as shown in SEQ ID No.1, a nucleotide sequence of the mutant downstream primer is as shown in SEQ ID No.2, a nucleotide sequence of the common upstream primer is as shown in SEQ ID No.3, and a nucleotide sequence of the detection probe is as shown in SEQ ID No.4. The kit and method for real-time fluorescent quantitative PCR rapid detection of C3435T polymorphism of the ABCB1 gene have the advantages of being convenient to use, simple to operate and high in automatic degree, greatly simplifying the operation process and reducing pollution in the operation process, and the detection effect is good; the kit has the characteristics of high sensitivity, specificity, accuracy and precision.

The invention provides a multifunctional sputum suction device for respiratory medicine. The multifunctional sputum suction device comprises a sputum suction catheter and a cup body, wherein the sputum suction catheter is connected to the upper part of the cup body and comprises a sputum reducing tube with a sputum diluting function, a flow meter with a sputum flow counting function and a hose forconnecting the sputum reducing tube with the flow meter, a sputum collection and detection bottle used for collecting sputum and detecting types of bacteria and viruses contained in the sputum is arranged in the cup body and communicates with the sputum suction catheter through a sputum channel in the cup body, and a driving device is further arranged in the cup body and used for enabling the sputum channel to be in a low-pressure state, so that the sputum suction catheter can suck the sputum. According to the multifunctional sputum suction device, microorganisms contained in sucked sputum can be detected under the condition that the sucked sputum is not polluted by the external environment, meanwhile, the sputum amount of a patient is counted, and better treatment can be conducted according to sputum information.