The invention discloses a transmission protocol compliance detection device, system and method. Parsing on various current general protocols is implemented, and a private protocol is subjected to identification detection by the flow of feature extraction, feature loading and feature string matching; a detection result can be subjected to showing, statistics and report export by a uniform interface; a user interface is provided, and the transmission protocol compliance detection device, system and method have functions of defining a new protocol, starting or stopping detection, uploading a file, showing the detection result, exporting the detection result and the like; receiving on a user command and a configuration from Web is implemented; when a detection command is executed, the new protocol is detected according to a defined new standard; and newly added protocol features can be directly subjected to dynamic defining and loading, so that the protocol identification expansion abilityis greatly improved, fine management control on the standard compliance test flow and scientific and fair evaluation on a compliance analysis result are implemented, standardized management of construction of the information communication field is effectively supported and propelled, and the interconnection ability between a network system and equipment is promoted.