154 results about "Genetic traits" patented technology

Blood plasma of pregnant women contains fetal and (generally>90%) maternal circulatory extracellular DNA. Most of said fetal DNA contains ≦500 base pairs, said maternal DNA having a greater size. Separation of circulatory extracellular DNA of <500 base pairs results in separation of fetal from maternal DNA. A fraction of a blood plasma or serum sample of a pregnant woman containing, due to size separation (e.g. by chromatography, density gradient centrifugation or nanotechnological methods), extracellular DNA substantially comprising ≦500 base pairs is useful for non-invasive detection of fetal genetic traits (including the fetal RhD gene in pregnancies at risk for HDN; fetal Y chromosome-specific sequences in pregnancies at risk for X chromosome-linked disorders; chromosomal aberrations; hereditary Mendelian genetic disorders and corresponding genetic markers; and traits decisive for paternity determination) by e.g. PCR, ligand chain reaction or probe hybridization techniques, or nucleic acid arrays.

A candidate single nucleotide polymorphism (SNP) combination is selected from a plurality of candidate SNP combinations for a gene associated with a genetic trait. Haplotype data associated with this candidate SNP combination are read for a plurality of individuals and grouped into a positive-responding group and a negative-responding group based on whether a predetermined trait criteria for an individual is met. A statistical analysis on the grouped haplotype data is performed to obtain a statistical measurement. The acts of selecting, reading, grouping, and performing are repeated as necessary to identity the candidate SNP combination having the optimal statistical measurement. In one approach, a directed search based on results of previous statistical analysis of SNP combinations is performed until the optimal statistical measurement is obtained. In addition, the number of SNP combinations selected and analyzed may be reduced based on a simultaneous testing procedure.

A method for screening for a trait associated with the altered expression of a gene of interest in plants is provided. The method is a combinatorial approach which uses traditional plant breeding techniques for modifying the patterns of expression of a gene of interest. Kits for use in the method and transgenic plants generated by the method are also provided.

Methods and systems are provided for managing companion animal subjects in order to maximize their individual health and potential performance and to maximize profits obtained in breeding and marketing the companion animal subjects. The methods and systems draw an inference of a phenotype for a genetic trait of a companion animal subject by determining the nucleotide occurrence of at least one companion animal SNP that is determined to be associated with the phenotype. The methods and systems described can be utilized to identify individual animals, determine or verify parentage of a single dog from any breed if the putative parent(s) are also available for testing, and are associated with, and predictive of, canine breeds. The inference is used in some aspects to diagnose a health condition or predisposition of a companion animal subject.

Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species. as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems.

The invention relates to a rapid propagation method for tissue cultivation of a dendrobium candidum stem, belonging to the technical field of artificial cultivation of dendrobe. The method comprises the following steps of preparing an explant, inducing axillary bud and protocorm, conducting propagation cultivation, and rooting and seeding cultivation. The rapid propagation method has the beneficial effects that the technical problems of insufficient explant sterilization, high pollution rate, incapability of conducting axenic cultivation, slow protocorm differentiation, less differentiation, long propagation period, high production cost and the like can be well solved. Compared with the prior art, the rapid propagation method has the advantages that rapid propagation can be achieved; and the propagation period can be reduced by over 60 days. In addition, according to the rapid propagation method, seeding genetic traits are stable, the variation is small, the variety is pure, and the parent characteristics and features can be well kept. Thus, excellent seeding guarantee is provided for development of plant industry of dendrobium candidum, and the rapid propagation method has good economic benefit and social benefit.

The invention relates to a seed tissue culture propagation method of dendrobium candidum and belongs to the technical field of planting of traditional Chinese medicinal materials. The seed tissue culture propagation method of dendrobium candidum comprises the following four steps of: introduction and sterilization, induction culture, differentiation culture as well as strong seedling and rooting culture. The seed tissue culture propagation method of dendrobium candidum has the beneficial effects that seedlings obtained by seed tissue culture are difficult to degenerate, genetic traits are stable, active substance content is close to that of a wild variety, and quality of seeds is guaranteed; quantity of the seeds of dendrobium candidum is high, and propagation quantity of the seeds can be greatly increased; the seed tissue culture propagation method of dendrobium candidum also greatly shortens a tissue culture propagation period of dendrobium candidum, the tissue culture period from seed shearing to rooting and seedling growing is only 4-5 months, production time is greatly shortened, and production cost is reduced.

The invention discloses a cutting and seedling method for phoebe shearer. The cutting and seedling method includes the steps: treating a cutting bed, namely paving cobblestones under a cutting bed and a layer of screen on the cutting bed and paving clean river sand for sterilization and disinfection of soil; collecting and producing cutting slips, namely clipping semi-lignification branches which are normal in growth with full shoots and without diseases and insect pests as cuttings from a phoebe shearer seed tree of 5-8 years from March to April to produce cutting slips; subjecting the produced cutting slips to early stage treatment and cutting; performing management after cutting. The cutting and seedling method is simple in operation, high in survival rate and capable of providing fine phoebe shearer seedlings, shortening breeding time of fine seeds and protecting excellent genetic traits of the seedlings. Therefore, propagation coefficient is greatly increased, and the survival rate reaches 80% at least.

The invention belongs to the field of establishment of a population genetic resource bank, and provides a scientific establishment method of a goat resource population bank, and provides a goat reference family system establishment case, and relates to two national-level genetic resource varieties, namely, a Dazu black goat and an Inner Mongolia Cashmere goat (Alxa type). According to the resource population establishment method, forward cross and reciprocal cross of Dazu black goat and Inner Mongolia Cashmere goat (Alxa type) are utilized, random mating of genetic relationship is avoided for F1, so that Mendel genetic characters are separated, a goat economic character QTL or a functional gene is located by virtue of a genome means, and a relatively-precise genetic mark is provided for the goat breeding. The genetic resource bank comprises goat population, a genealogy inforamtion database, a phenotypic character database, a DNA sample library, an RNA sample library and a genetic variation information database.

The present invention relates to a kind of culture medium composition suitable for blueberry test-tube seedling proliferation and method thereof, the steps are as follows: 1) culture medium comprises basic culture medium and each component of culture medium of each stage of tissue culture and the contained weight per liter is: ( 1) Basic medium: WPM, including sugar 30g/L, agar 7~8g/L, pH5.3; (2) Induction medium: WPM+TDZ1.0~2.0mg/L+IBA0.3~0.5mg/L L; (3) leaf induction medium: WPM+TDZ2.0~3.0mg/L; (4) proliferation medium: WPM+TDZ0.2~0.5mg/L+IBA0.1~0.2mg/L+GA0. 1mg/L. 2) Selection and sterilization of explants; 3) Induction culture: directly induce the primary shoots from the explants; 4) Culture on the leaf induction medium to form embryogenic callus or directly form a complete plant Regeneration of adventitious buds; 5) Proliferation culture. The beneficial effects of the invention are as follows: 1) the culture medium has strong pertinence and good applicability, and the bud induction rate is high; 2) the multiplication coefficient is high, and the tissue culture cost is saved. 3) The genetic traits are consistent, which overcomes the disadvantage of low conventional reproduction coefficient.

Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of a cultivated crop to an undesirable, interbreeding related species.

The invention relates to the field of the aquiculture, in particular to methods of the breed selection of the soft-shelled turtle breed-parent and the breeding of the soft-shelled turtle. The breed selection of the soft-shelled turtle includes obtaining F1 by crossbreeding of the soft-shelled turtle individual and the strip-necked turtle; obtaining the soft-shelled turtle breed-parent F4 after breed selection of three generations, the genetic character thereof is stable at above 99%. The invention also discloses the breeding of the soft-shelled turtle, which includes introducing and breeding F4 as the breed-parent; egg-laying, egg taking, incubating, temporarily breeding of young turtles, breeding of baby turtles, stocking in the outer pond and obtaining the soft-shelled turtle. The bred soft-shelled turtle is characterized in good taste, good mouth feeling and high nutritive value.

The invention provides genetic engineering strain Streptomyces tsukubaensis L21. The fungus preservation unit is CGMCC (China General Microbiological Culture Collection Center), the preservation date is August 19, 2015, and the preservation number is CGMCC No. 11253. According to the genetic engineering strain Streptomyces tsukubaensis L21, the strain genetic character and the fermentation unit are stable, culture and fermentation conditions are suitable for industrial production, and the fermentation unit is high. The copy number of specific controlling genes in the Tacrolimus creature biosynthetic pathway is increased through genetic engineering, and the genes improve rate-limiting enzyme in the Tacrolimus creature biosynthetic pathway so as to increase the Tacrolimus yield, reduce the cost in the industrial production process and improves the Tacrolimus fermentation unit to 945 mg/L.

The invention discloses a typing method of resistance for resisting a subgroup A avian leukosis virus by quality chickens, which adopts an SNP locus at the 619th site of a TVA gene sequence as a research object; PCR-RLFP analysis is performed to detect whether mutation occurs at the SNP locus, and also to detect whether 4 bases are inserted into SNP loci at the 305th-308th sites of the TVA gene sequence; the two detection results are combined together to determine whether the detected chicken is a genetic resistance type or a genetic susceptibility type. The resistance typing method of the invention performs detection from the source, and omits troubles of later-stage detection; the detection method is rapid, accurate, strong in purposiveness, and simple in operation, has practice significance, and can realize rapid selection of chicken genetic characters and improvement of disease resistance of strains.

The invention discloses a tissue culture and rapid propagation method of eucalyptus urophylla DH33 to DH27 varieties. The method comprises the following steps: establishing an explant, sterilizing the explant, carrying out primary culture, carrying out secondary culture, carrying out rooting culture, exercising seedlings, transplanting and the like. A special culture medium is selected for the primary culture, the secondary culture and the rooting culture, the eucalyptus urophylla can grow fast and is high in survival rate due to the reasonable seedling exercising means, and the purposes of high propagation coefficient and good propagation effect can be achieved. Meanwhile, the method is low in production cost, the genetic traits of the obtained tissue-culture seedlings are consistent, the method is an effective way for the tissue culture and rapid propagation of the eucalyptus, and the application range is wide.