39results about How to "High pollution rate" patented technology

The invention provides a method for cultivating plant tissue under non-aseptic condition, wherein the whole procedure of plant tissue cultivation is conducted under the natural ambient condition of non-asepsis, the method consists of adding disinfection and bactericidal agent into the culture media, preparing bacterium suppression culture media, inoculating under the natural ambient condition of non-asepsis, and placing the culture material in natural ambient condition meeting plant tissue culture.

The invention provides a cultivation method of black fungus. The cultivation method comprises the steps of: (1) preparing a culture material, uniformly mixing crushed sea-buckthorn twigs, sunflower heads, maize meal, bran, oil residues, lime and gypsum in formula ratio, regulating pH value to 8.5, and sterilizing; (2) inoculating, culturing hyphae, and cleaning fungus; (3) moving fungus bags to a previously disinfected cultivation field with temperature of 22-28 DEG C for fruiting cultivation; (4) picking finished products when black fungus sporocarp grows to ripe at eighty percent to obtain the black fungus. The cultivation method provided by the invention can not only increase the output of the black fungus, but also increase the total flavonoid content of the black fungus through measurement. In addition, compared with common field cultivation black fungus, the black fungus has the advantages of short period, low cost, less pollution, uniformity in fungus growth, standardization and industrialization.

The invention discloses a boiler ash-blowing optimization system and a boiler ash-blowing optimization method and relates to the technical field of boiler ash-blowing. The boiler ash-blowing optimization system comprises a DCS (distributed control system) and a monitoring system, wherein the DCS is in communicated connection with the monitoring system, and the monitoring system comprises a water-cooling wall ash deposition monitoring module based on a radiation mode, a horizontal flue heat-exchange area ash monitoring module based on a convection mode and an air preheater ash deposition monitoring module. An automatic ash-blowing decision suggestion system based on the DCS is established to be communicated with a corresponding DCS server, pollution rates of heated surfaces are computed and analyzed in real time, a high pollution rate limit is obtained through further experiments, and light indicator warning or an automatic ash-blowing command is achieved once the high limit is obtained, so that ash-blowing operation in actual running is guided effectively.

The invention discloses a production technology of gardening edible mushroom blocks. The technology comprises the following steps that water is added to a culture medium, stirring is carried out, and full-automatic bag sleeving, bagging and block pressing are carried out; high-pressure sterilization is carried out for 60 min-120 min under the temperature of 105 DEG C-130 DEG C, and then cooling is carried out to room temperature; full-automatic inoculating machine inoculating is carried out, inoculating temperature is 5 DEG C-15 DEG C, and inoculating time is 2 s-5 s per block; a continuous sealing machine is used for heat sealing; and under the condition that temperature is 16 DEG C-26 DEG C, humidity is 60%-85%, and carbon dioxide concentration is 500 ppm-3000ppm, mycelium cultivation is carried out. According to the technology, an edible mushroom bag with a ventilation hole and a ventilation film is used, external contaminating microorganisms are prevented from invading to the maximum degree, and cultivation contaminating rate is lowered to 0.1% from current 5%; and the problem that mechanical operation cannot be achieved on bag cultivation in a bagging link and an inoculation link is solved, mechanical production of the whole technology is achieved, labor cost of a whole production process can be lowered by 50%, and production efficiency is greatly improved.

The invention discloses an induction method of the mature embryo callus of syringa microphylla, relates to a callus culture method, and particularly relates to an induction method of the mature embryo callus of syringa microphylla. The invention aims at solving the problems of high pollution rate and low induction rate of the callus induction using stems, leaves and root tips. The method disclosed by the invention comprises the following steps: (I) disinfection and inoculation of explants; (II) induction of callus. According to the method disclosed by the invention, the explant collection period is centralized, the pollution rate is controlled very low within 1%, and the induction rate of the syringa microphylla callus is high over 75%. The method disclosed by the invention is applied to callus induction.

The invention provides a tissue culture and rapid propagation method for paphiopedilum dianthum. The tissue culture and rapid propagation method comprises the following steps: (1) carrying out self pollination; (2) picking pods; (3) carrying out asepsis sowing; (4) carrying out propagation subculture; (5) carrying out rooting and strong seedling culture; and (6) transplanting rooted plantlets. The invention also provides a culture medium system applicable to the tissue culture and rapid propagation of the paphiopedilum dianthum, and the culture medium system comprises a germination culture medium, a propagation culture medium and a rooting and strong seedling culture medium and can be applied to the tissue culture and rapid propagation method of the paphiopedilum dianthum. The invention also provides the best picking period of paphiopedilum dianthum pods, namely the pods are picked after the self pollination of the paphiopedilum dianthum is carried out for about 270-330 days. Seedlings cultured by adopting the method provided by the invention are high in germination rate, robust, developed in root system and short in growth cycle; meanwhile, the method provided by the invention is simple and easy to operate and is low in production cost, and a good foundation is provided for commercial production of the paphiopedilum dianthum.

The invention discloses a method for culturing and propagating tissues of Taxodium Zhongshanha 302. The method comprises the following steps: (1) selecting an explant and disinfecting the explant; (2) improving an original WPM (wood plant medium) culture medium; (3) after cutting the processed explant, performing primary induction and culture for stems; (4) performing secondary multiplication andculture for new tips obtained in the step (3); (5) performing rooting induction culture for the rootless test-tube plantlet obtained in the step (4); and (6) performing domesticating hardening off culture for the aseptic seedlings obtained in the step (5). The propagating method is adopted to culture seedlings of the Taxodium Zhongshanha 302, the propagating rate is 102-110 times and the seedlingculturing time is short.

The invention relates to the field of separation of stem cells, in particular to an endometrial stem cell isolation method, comprising the following steps: after blood plasma is removed from a sample,conducting diluting, conducting filtering by a sieve of not more than 100 [mu]m, adding antibiotics into an obtained filtrate, then conducting low-temperature treatment, conducting washing and separating, and culturing obtained cells to obtain first endometrial stem cells; washing endometria on the sieve, then adding a red blood cell lysate, conducting separation on lysed endometrial, then conducting shearing, digestion and separation, culturing obtained cells to obtain second endometrial stem cells; and combining the first endometrial stem cells and the second endometrial stem cells to obtain endometrial stem cells. According to the endometrial stem cell isolation method provided by the invention, menstrual blood-derived endometrial stem cells and exfoliated endometrium-derived cells areseparated, more endometrial stem cells are effectively obtained, and the endometrial stem cells meet a clinical use standard through flow cytometry detection.

The invention provides a fermenting agent suitable for edible fungi and a method for fermenting the edible fungi by using the fermenting agent, and relates to the technical field of edible fungus planting. The fermenting agent suitable for the edible fungi is prepared from 97-99wt% of main materials and 1-3wt% of auxiliary materials; the main materials comprise cottonseed hulls, roxburgh rose residues, honeysuckle straws and crop straws, and the weight ratio of the cottonseed hulls to the roxburgh rose residues to the honeysuckle straws to the crop straws is 0.8: 0.6: 0.3: 0.3; and the auxiliary materials comprise lime, gypsum and a fermentation auxiliary agent, and the fermentation auxiliary agent is an 1-3 wt% EM aqueous solution. The invention further provides a method for fermenting the edible fungi by using the fermenting agent. According to the method, exogenous heat is introduced through a vent pipe and cooperates with the fermentation materials for rapid fermentation, so that the fermentation time is greatly shortened, the field is saved, labor is saved, energy consumption is reduced, and the economic efficiency is improved.

The invention relates to a preparation method of a cordyceps militaris coarse food porridge material, and belongs to the technical field of production of foods. The preparation method comprises the following steps of using a mixed material of avena sativa kernel, brown rice, sweet corn, yellow bean and lotus seeds as a culture medium of the coarse food, and using a mixed solution of white granulated sugar, milk powder and water as a nutrient solution; mixing the nutrient solution and the culture medium of the coarse food, sterilizing, inoculating a cordyceps militaris liquid strain, and culturing cordyceps militaris sporocarp; after the cordyceps militaris sporocarp fully grows on the to-be-harvested culture medium surface, fetching out the cultured substrate, drying, crushing, packaging under the vacuum condition, radiating and sterilizing, so as to obtain the cordyceps militaris coarse food porridge material. The preparation method has the advantages that the high-quality cordyceps militaris sporocar is obtained, the substrate after culturing is fully used for simple processing, and the edible coarse food porridge material with higher nutritional value is obtained.

The invention provides a tissue culture and rapid propagation method of actinidia deliciosa root stocks, and belongs to the technical field of plant tissue culture. According to the method, through technical innovation of explant disinfection, adventitious bud regeneration and induction, proliferation, rooting culture and the like, the pollution rate of explants is extremely low, the problem of explant browning is effectively solved, tissue culture seedlings grow strongly, and the growth speed of the tissue culture seedlings is obviously increased. In addition, the method disclosed by the invention not only simplifies working procedures and saves times, but also reduces pollution, and is suitable for industrialized production needs.

The invention provides a transplanting method for tissue culture seedlings of kiwi fruit rootstocks, and belongs to the technical field of plant tissue culture propagation. The method comprises the steps that tissue culture cluster buds of Jinkui of the delicious kiwi fruits subjected to multiplication culture are washed clean, cut open and separated into single seedling plants, then sterilizationtreatment is conducted, the lower ends of the single seedling plants which do not take roots are evenly covered with rooting live root powder, the single seedling plants are directly inserted into aseedling culture plug filled with a matrix for normal seedling culture, and the single seedling plants are transplanted to a nursery after two months. Rooting agent treatment is adopted to replace rooting culture and seedling hardening treatment, through control management in the transplanting process, the relatively high transplanting survival rate is achieved, and meanwhile, the kiwi fruit tissue culture propagation time is greatly shortened, and the seedling culture cost is greatly reduced.

The invention relates to the technical field of tissue culture and high-throughput breeding of seedlings, in particular to a high-throughput breeding method for breadfruit seedlings. The method comprises the following steps: performing cuttage on root sections of breadfruit mother plants, taking stem sections growing on the root sections to prepare explants, and cleaning, disinfecting and sterilizing the explants; inoculating a callus induction culture medium with the explants for callus induction to obtain stem segments with callus; inoculating a cluster bud induction culture medium with the stem segments with the callus for proliferation and differentiation of cluster buds to obtain cluster buds; and dividing the cluster buds, and inoculating a rooting culture medium with the cluster buds for rooting to obtain complete plants. According to the method, the root sections of the mother plants are used for cuttage, and the stem sections growing on the roots are used as explant materials, so that the cluster buds can be induced to obtain the cluster buds.

The invention discloses a phellinus linteus sporocarp hypha separating method. The method comprises the steps of placing complete phellinus linteus sporocarp into a culture bottle; adding liquid until the phellinus linteus sporocarp is immersed; boiling and maintaining for 5 to 20 seconds; culturing for 8 to 72 hours at the temperature of 25 to 37 DEG C; repeating the boiling and culturing processes for a plurality of times until hypha grows in the phellinus linteus sporocarp; separating hypha. Compared with the traditional tissue separation method, the phellinus linteus sporocarp hypha separating method has the characteristics that the raw materials are easily obtained, the preparation is convenient, special devices are saved, the contamination rate in operation is low, the success rate is high, the hypha can grow quickly, and the culture costs a little time; the method is very suitable for field separation of the phellinus linteus sporocarp hypha.

The invention provides a kiwi explant detoxification method. The method sequentially comprises the following steps: explant selecting: removing leaves from kiwi young seedlings, intercepting a buddedstem segment of 1cm to 2cm, cleaning and disinfecting the budded stem segment, sucking up the budded stem segment with sterile filter paper, and stripping explant meristem; induced culture: inoculating an induction culture medium with the explant meristem, and carrying out dark culture prior to light-dark culture, so as to obtain clustered buds; rooting culture: transferring the clustered buds toa rooting culture medium when the height of the clustered buds reaches 1cm to 2.5cm and the clustered buds are accompanied with 2 to 5 young leaves, and carrying out light-dark culture, so as to obtain tissue culture seedlings; and seedling hardening transplanting: culturing the tissue culture seedlings, transplanting the cultured tissue culture seedlings into a mixed matrix, and carrying out culturing, thereby obtaining virus-free seedlings. According to the method, robust virus-free seed seedlings are obtained sequentially through explant selecting, induced culture, rooting culture and seedling hardening transplanting, the production cost is reduced, the quality of nursery-grown plants is improved, the problems in the prior art that kiwi seed seedlings carry viruses and the virus carrying rate is relatively high are effectively solved, and large-area popularization of kiwi planting industry is facilitated.

The invention provides a method for cultivating a passionflower sterile explant, and relates to the technical field of plant tissue culture. The method comprises the following steps: collecting a passionflower tender stem segment as an explant, washing the explant, then soaking the explant in a saturated washing powder solution, and rinsing the explant to obtain a pretreated explant; carrying out sterile pre-culture on the pretreated explant by adopting a rifampicin solution to obtain a pre-cultured explant; cutting the pre-cultured explant into stem segments, and disinfecting the stem segments to obtain sterile stem segments; and culturing the sterile stem segments in an MS basic culture medium to obtain the sterile passionflower explant. According to the cultivation method disclosed by the invention, by combining a two-time pretreatment method of the explant, a mercury bichloride sterilization method, time and the like, the pollution rate is reduced, and meanwhile, the browning rate and the death rate caused by disinfection and sterilization are also reduced, so that the survival rate of the explant is greatly increased, and the production cost of tissue culture is reduced.

The invention discloses a method for multiplying vetiver seedlings by means of calluses. The method comprises the steps of culture medium preparation, explant selection, explant disinfection, explant inoculation, tissue culture seedling culture, seedling transplanting culture medium preparation and seedling separation and transplanting. By means of the method for multiplying the vetiver seedlings by means of the calluses, callus induction and callus differentiation germination and rooting can be completed; meanwhile, culture medium components are optimized, induction efficiency is improved, and eustipes and leafbud callus induction efficiency is improved, wherein the efficiency can reach up to 88.3% counted by the number of the initial inoculating explants; three cultivation links of callus induction and callus differentiation germination and adventitious bud rooting can be completed through one-time inoculated culture, transfer is not needed during the process, sundry bacteria contamination is reduced, and the seedling rate is increased, wherein the seedling rate exceeds 70% and reaches up to 73% counted by the number of the initial inoculating explants.

The invention relates to a shiitake mushroom substitute cultivation substrate and a preparation method thereof, belonging to the technical field of edible fungus cultivation, and is composed of the following proportioned raw materials in parts by weight: 30% to 50% of mulberry twig shavings nutrient material, 30% to 50% of hazelnut shell matrix particles %, 20% decomposed dry material of sesame paste residue, 0.5% to 1% of sheep bone calcification powder, 0.5% of quick-acting fungicide, and 0.1% of mushroom element. Through microbial fermentation, degradation and high-temperature calcination, the nutritional components in crops such as mulberry twigs, hazelnut shells, and sesame paste residues and leftovers in the processing process are fully utilized, and the fungus bags prepared by reasonable proportioning have good air permeability , the pollution rate of mushroom sticks is significantly reduced, the cycle of shiitake mushrooms is significantly shortened, the biological efficiency of shiitake mushrooms is significantly improved, and the aroma is unique, the taste is delicious, and the value of nutrition and health care is excellent.

The invention discloses a method for multiplying vetiver seedlings by means of calluses. The method comprises the steps of culture medium preparation, explant selection, explant disinfection, explant inoculation, tissue culture seedling culture, seedling transplanting culture medium preparation and seedling separation and transplanting. By means of the method for multiplying the vetiver seedlings by means of the calluses, callus induction and callus differentiation germination and rooting can be completed; meanwhile, culture medium components are optimized, induction efficiency is improved, and eustipes and leafbud callus induction efficiency is improved, wherein the efficiency can reach up to 88.3% counted by the number of the initial inoculating explants; three cultivation links of callus induction and callus differentiation germination and adventitious bud rooting can be completed through one-time inoculated culture, transfer is not needed during the process, sundry bacteria contamination is reduced, and the seedling rate is increased, wherein the seedling rate exceeds 70% and reaches up to 73% counted by the number of the initial inoculating explants.

The invention discloses bulk food packaging equipment capable of increasing packaging efficiency, and relates to the technical field of packaging equipment. The bulk food packaging equipment capable of increasing the packaging efficiency comprises a table, wherein the upper surface of the table is fixedly connected with the lower surface of a material box through two support plates. According to the bulk food packaging equipment capable of increasing the packaging efficiency, through the mutual cooperation of a first switch, a stirring rod, an electronic scale, a pedal, a rope, a pulley, a tooth plate, a full gear, a first rotary shaft, a drive wheel, a driven wheel, a valve and a torsional spring, working personnel do not need to stir and mix at first, then shovel bulk foods into a packaging box by virtue of a small shovel, then place the packaging box onto the electronic scale and weigh, and then increase or reduce the bulk foods according to the actual need of a customer, so that the labor intensity of the working personnel is lowered, the pollution of the manual operation of the working personnel on the foods is further reduced, and then the quality of the products is improved,the packaging efficiency of the working personnel for the foods is increased, and convenience is brought to the packaging of the working personnel for the foods.

The invention provides a boiler water wall ash pollution monitoring system. N soot blowers are arranged on a boiler water wall, N is larger than or equal to 1, the N soot blowers are located at M different heights, M is larger than or equal to 1, n soot blowers are arranged at each height, and n is larger than or equal to 1 and smaller than or equal to N. The monitoring system is characterized bycomprising J fin thermocouples, K pipe wall thermocouples, a temperature data collector and a data processing platform. The fin thermocouples are arranged near the soot blowers, on one hand, the number of the required fin thermocouples is small, and on the other hand, once it is monitored that the pollution rate of the water wall area where the thermocouples are located is high, the soot blowers near the thermocouples can conduct soot blowing operation under control, the heating surface is kept clean, and the soot blowing effect is monitored.