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Method for multiplying vetiver seedlings by means of calluses

A technology for callus and vetiver grass, applied in the field of plant reproduction, can solve the problem that the pollution rate in the cultivation process is not easy to control, and achieve the effects of optimizing the composition of the medium, reducing the probability of pollution, and reducing the number of transfers.

Inactive Publication Date: 2016-06-08
NANYANG NORMAL UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for propagating vetiver seedlings by using callus, aiming to solve the problem that the pollution rate in the cultivation process is not easy to control in the existing method for propagating vetiver seedlings

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  • Method for multiplying vetiver seedlings by means of calluses
  • Method for multiplying vetiver seedlings by means of calluses
  • Method for multiplying vetiver seedlings by means of calluses

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Embodiment Construction

[0021] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] In the method of the invention, only one culture medium is used in the seedling induction process to complete callus induction, callus differentiation, sprouting and rooting, without transfer in the middle, thus reducing the probability of contamination. At the same time, the method also optimizes the composition of the medium and improves the induction efficiency.

[0023] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0024] Such as figure 1 Shown, the method for utilizing callus propagation vetiver seedling of the embodiment o...

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Abstract

The invention discloses a method for multiplying vetiver seedlings by means of calluses. The method comprises the steps of culture medium preparation, explant selection, explant disinfection, explant inoculation, tissue culture seedling culture, seedling transplanting culture medium preparation and seedling separation and transplanting. By means of the method for multiplying the vetiver seedlings by means of the calluses, callus induction and callus differentiation germination and rooting can be completed; meanwhile, culture medium components are optimized, induction efficiency is improved, and eustipes and leafbud callus induction efficiency is improved, wherein the efficiency can reach up to 88.3% counted by the number of the initial inoculating explants; three cultivation links of callus induction and callus differentiation germination and adventitious bud rooting can be completed through one-time inoculated culture, transfer is not needed during the process, sundry bacteria contamination is reduced, and the seedling rate is increased, wherein the seedling rate exceeds 70% and reaches up to 73% counted by the number of the initial inoculating explants.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for propagating vetiver seedlings by using callus tissue. Background technique [0002] Vetiver (Vetiveriazizanioides L.), also known as pedima and vetiver, is a perennial herbaceous plant of the Gramineae family. It originated in India and other countries, and is now mainly distributed in (sub)tropical regions such as Southeast Asia, India and Africa. my country is mainly distributed in Guangdong, Yunnan and other places. Vetiver is extremely difficult to bear fruit or does not bear fruit. It mainly reproduces by tillers. That is, every spring, the roots of vetiver left in the field in the previous year will germinate new tillers. Generally, each pocket of vetiver roots can grow 40% in spring. Tillers around the plant. Theoretically speaking, each tiller can grow into an independent stem with 6-10 nodes. In fact, only the larger tillers (with an a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈吉宝曹苑南增辉董海鸿李丹丹
Owner NANYANG NORMAL UNIV
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