Sinorhizobium meliloti strain and composition and application of sinorhizobium meliloti strain

Abstract

The invention discloses a sinorhizobium meliloti strain capable of being applied to fermentation to generate vitamin B12, wherein the preservation number of the sinorhizobium meliloti strain is CGMCC No.9638. The invention also discloses a production and preparation method of vitamin 12 by the sinorhizobium meliloti strain in a fermentation manner. The method comprises a fermentation culture condition of the strain and an extraction method of the vitamin 12 in fermentation liquor; the method comprises the following steps: inoculating the strain on a solid culture medium into a triangular flask as a seed solution; inoculating the seed solution into a fermentation culture medium at 30 DEG C with 10% of inoculation amount; fermenting and cultivating at 200rpm for 200 hours, and then collecting the fermentation liquor; and adding a transforming agent to transform the fermentation liquor into cyano cobalt amine. The vitamin 12 is widely applied to the industries such as feeds, additives, medicines and cosmetics as a special factor in an adjusting organism growth process; and the requirement of the vitamin 12 rises year by year. The strain fermentation process is easy to control, so that the strain fermentation process is not affected by the factors such as seasons; the requirements on raw materials are simple; and fermentation production is developed into a main production mode.

Description

technical field [0001] The invention belongs to the technical fields of biological resources, microbial fermentation and macromolecular natural product biosynthesis. Involves a strain producing vitamin B 12 The strain, Sinorhizobium meliloti ( Sinorhizobium medicae ), and use the strain to ferment and prepare vitamin B 12 The method, more specifically a Sinorhizobium meliloti strain and its composition and application. Background technique [0002] Vitamin B 12 Also called cobalamin (Cobalamin), which belongs to corrin compounds, is currently the only vitamin compound containing metal ions, from vitamin B 12 It can be seen from the structure that its basic structure includes a corrin ring and a ligand. The central corrin ring is composed of 4 pyrroles connected to 1 cobalt atom, and the cobalt is chelated at the 4 pyrrole centers. Different types of ligands above the corrin ring form different types of cobalamins, generally four types can be formed: (1) Cyanocobalamin ...

AI Technical Summary

Problems solved by technology

However, microbial detection takes a long time and has low sensitivity, which is not conducive to detection during fermentation; colorimetry is only suitable for the analysis of samples with relatively simple components such as drugs, but it has interference effects on samples with fermentation or complex components; Calculation of changes in vitamin B 12 Although the detection method is accurate and fast, the sample itself contains cobalt, which will cause a large error; due to the low detection limit of high-efficiency capillary electrophoresis, it will cause trace amounts of vitamin B in food, etc. 12 The detection has limitations; TLC has limitations in quantitative detection due to the inconsistent loading amount; high performance liquid chromatography can detect vitamin B in samples with low content and complex components 12 content, with the advantages of simplicity, speed and accuracy

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