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Tissue culture and rapid propagation method for paphiopedilum dianthum

A technology of tissue culture and Papudendron longiflorum, applied in the field of plant tissue culture, to achieve the effect of simple medium formula, strong growth of seedlings, simple and easy-to-operate operation process

Active Publication Date: 2017-06-13
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yet in the prior art, there are few reports about the tissue culture technology of P. longiflorum, only Wang Lianhui et al. ): 887-888) carried out aseptic sowing and tissue culture rapid propagation experiments on Papudlum longiflorum, and proved that the medium of 1 / 2MS+100mL·L-1 coconut milk or MS+100mL·L-1 coconut milk is more suitable Seed germination can reach more than 60%; 1 / 2MS+6-BA 0.2+NAA 1.0+100mL·L-1 coconut milk is the best proliferation subculture medium, which can be subcultured once every 80-90 days; 1 / 2MS+indole Inolebutyric acid (IBA) 0.2+2g·L-1 activated carbon is a better rooting medium

Method used

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  • Tissue culture and rapid propagation method for paphiopedilum dianthum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1. Determination of self-pollination and fruit pod optimum harvesting period of P.

[0060] Experimental materials: Papudendron longifolia was obtained from the greenhouse of Vegetable and Flower Research Institute, Chinese Academy of Agricultural Sciences; various reagents or medium components used in all the examples of the present invention were obtained commercially.

[0061] Select the large and colorful Paphiopedilum longifolia for artificial cross-pollination, such as figure 1 As shown, pods were grown after pollination.

[0062] After pollination, pods were collected on the 240th day, the 270th day, the 300th day, the 330th day, the 360th day, the 390th day and the 420th day, respectively.

[0063] After the above-mentioned fruit pods were washed with tap water, they were placed in 10% sodium hypochlorite solution for disinfection for 20 minutes, sterilized with 70% alcohol for 30 seconds, then sterilized with 0.1% mercury liter for 10 minutes, and...

Embodiment 2

[0067] Embodiment 2. Determination of the optimal germination medium of Papudendron longifolium seeds

[0068] Eight different germination media were designed, and the existing MS medium in the prior art was supplemented with sucrose and carrageenan (germination medium No. 9) as a control, as shown in Table 4.

[0069] Table 4. Components and contents of 9 media

[0070]

[0071]

[0072]

[0073] According to the method as described in Example 1, the seeds in the fruit pods collected on the 330th day after pollination were respectively inoculated in the 9 media in the above-mentioned Table 4 for germination and culture, and the germination of the seeds was observed.

[0074] As a result, it was found that the seeds of Paphiopedilum longum could germinate well in 8 kinds of germination media designed by the present invention, and the germination rate was significantly higher than that of the control medium, and the germination rate in No. ⑥ medium was the highest, r...

Embodiment 3

[0075] Embodiment 3. Determination of the best propagation medium for Papudendron longifolium seeds

[0076] Five different proliferation media were designed, as shown in Table 5.

[0077] Table 5. Proliferation and differentiation of the protocorm of P. longifolia on different proliferation media

[0078]

[0079]

[0080] The seedlings cultivated in Example 2 were inoculated into the above-mentioned proliferation medium, subcultured once every 4 weeks, and the proliferation of the seedlings was observed.

[0081] Proliferation culture was carried out for about 8 weeks, and it was found that the proliferation effect of seedlings in the second proliferation medium was good, protocorms grew on calluses, and the seedlings were robust.

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Abstract

The invention provides a tissue culture and rapid propagation method for paphiopedilum dianthum. The tissue culture and rapid propagation method comprises the following steps: (1) carrying out self pollination; (2) picking pods; (3) carrying out asepsis sowing; (4) carrying out propagation subculture; (5) carrying out rooting and strong seedling culture; and (6) transplanting rooted plantlets. The invention also provides a culture medium system applicable to the tissue culture and rapid propagation of the paphiopedilum dianthum, and the culture medium system comprises a germination culture medium, a propagation culture medium and a rooting and strong seedling culture medium and can be applied to the tissue culture and rapid propagation method of the paphiopedilum dianthum. The invention also provides the best picking period of paphiopedilum dianthum pods, namely the pods are picked after the self pollination of the paphiopedilum dianthum is carried out for about 270-330 days. Seedlings cultured by adopting the method provided by the invention are high in germination rate, robust, developed in root system and short in growth cycle; meanwhile, the method provided by the invention is simple and easy to operate and is low in production cost, and a good foundation is provided for commercial production of the paphiopedilum dianthum.

Description

technical field [0001] The present invention relates to the technical field of plant tissue culture, in particular, the present invention relates to a method for rapid propagation of P. The method of the best collection period for the pods of Phyllostachys chinensis. Background technique [0002] Paphiopedilum is one of the most characteristic and primitive groups of Orchidaceae. Paphiopedilum has high ornamental value due to its unique flower shape, gorgeous flower color and long-lasting flowering period. It is a very popular high-end flower in the international flower market and has a large number of lovers in the world. In recent years, more and more Paphiopedilum have appeared in orchid exhibitions and flower shows at home and abroad. With the improvement of people's living standards, more and more people begin to like and consume this precious orchid. The Paphiopedilum industry has quietly started. Many Paphiopedilum hybrids and native species have been able to underg...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01D91/04
CPCA01D91/04A01H4/001A01H4/008
Inventor 贾瑞冬葛红李秋香周妍慧贾雯慧徐玉凤王洪云刘娜
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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