100 results about "Self-pollination" patented technology

The invention relates to a method for producing parental lines for a hybrid organism. This method is based on the segregation of individual alleles in the spores produced by a desired plant and / or in the progeny derived from the self-pollination of that desired plant, and on the subsequent identification of suitable progeny plants in one generation, or in a limited number of inbred cycles. The invention further relates to a method for reconstructing a partially heterozygous starting organism.

The invention belongs to a cultivation method of apple bonsai realizing current-year fruiting and market-selling in Spring Festival. The method comprises the following steps: adopting the seedling of one to two-years old apple tree or fruit tree of similar variety as root stock in middle Feburary every year, wherein 55-62 cm of the seedling trunk is left upwards from the on-earth part, three to five main branches are left on the seedling trunk, and the length of the main branch is 10-20 cm; cultivating the root stock seedling on a ridge sweet potato insulating bed; selecting the flower buds on the short and dwarf old branch grown in the previous year of a 5-7 years old fruit tree as the scions, and grafting two different dwarf scions on each root stock seedling so as to realize permanent self-pollination; and controlling the growth temperature and conditions of the apple bonsai, sterilizing, disinsectizing, fertilizing, applying growth hormones to the apple bonsai and controlling the maturing and market-selling time of the apple bonsai. The cultivation method provided by the invention can cultivate the bonsai of apple or other fruits, is simple and easy to implement, has the advantages of low cultivation cost, fast growth and high success rate, and increases the bonsai varieties.

The invention discloses a cultivating and planting method of a begonia named Yinjiao. According to the invention, an artificial pollination method is adopted, begonia dryadis irmsch. is adopted as a female parent, B.'White King' is adopted as a male parent, and sexual hybridization is carried out; an offspring individual plant is subject to self-pollination, and the variety Yinjiao is obtained through selective breeding from an F2 generation colony; the chromosome numbers of the parents and the filial generation are determined; and the variety Yinjiao obtained through the previous cultivationsteps is planted in humic soil under a temperature of 15-25 DEG C and an air relative humidity of 65-75%, where in the humic soil is air-permeating and is rich in organic matters. Also, the drainage of the humic soil is good.

The invention discloses a method for rapidly breeding a sweet-waxy double-recessiveness maize selfing line. Self pollination or intraspecific free powder scattering is conducted on existing sweet-waxy maize varieties, seeds with the phenotype being sunken shrinking are selected from clusters obtained through selfing to be planted, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, the sweet-waxy selfing line does not need to be bred by oneself, adopted materials are simple and easy to obtain, operation is easy and convenient, cost is low, and the breeding term is short. The invention further discloses the method for rapidly breeding the sweet-waxy double-recessiveness maize selfing line. Different sweet-waxy maize varieties are respectively planted according to different sweet genes, the interspecific crossing free powder scattering is conducted on varieties the same in sweet gene, the seeds with the phenotype being sunken shrinking are selected, and the stable sweet-waxy double-recessiveness maize selfing line is obtained after continuous selfing. According to the method, gene polymerization is facilitated, the excellent sweet-waxy double-recessiveness selfing line is easier to select, and a good foundation is laid for widening the maize germplasm resources.

The invention relates to the field of plant breeding and specifically discloses a method for improving the self-pollination seed setting percentage of diploid potatoes. The method comprises: carrying out artificial self-pollination on diploid potato flower buds, preferably carrying out artificial self-pollination on the flower buds with a relatively low S-RNase gene expressive quantity. Self-incompatibility of the diploid potatoes is overcome by selecting a proper pollination time for self-pollination. The method is simple and feasible. No exogenous wild genomes are needed to be transferred while self-incompatibility is overcome, so that the obtained self-pollinated seeds can be directly applied to a process of genetic research and breeding.

A method for breeding a fine variety of peaches by bud mutation is composed of a breeding method of a good single plant, a grafting method and a management method. The breeding method of the fine variety of peaches is characterized in that the grafting method comprises the following steps: cutting a mature branch with plump leaf buds from the sunny side of a peach as a scion; soaking the cut scion branch in an imported benzatropine powder liquid of 500 folds for 5h, and then grafting a xylem bud to a common wild peach stock; fixing the grafted bud by a special grafting plastic film which is soaked in the imported benzatropine powder liquid of 500 folds for 10h. The method has the advantages of big fruits, fragrant and sweet taste, bright red and gorgeous appearance, beautiful appearance, indehiscence and no-drop after being mature, long harvesting time, hard solute, long-term storage and transportation without damage, wide adaptability, pollen, and self-pollination bearing capacity. The grafted peach has less disease and pest damages in the same year, good drought resistance and storage water logging resistance, and is a new peach variety with stronger stress resistance. Compared with the peach variety fruit of which become mature in the same term, the new variety can obtain higher economic benefit and better development prospect under the same management conditions.

The invention relates to the field of crop selection and crop breeding, particularly to selection methods for breeding colony varieties of crops involving self-pollination and normal cross-pollination. Selection methods include crossing male parents with female parents to obtain crop populations, wherein the female parents are individual plants in a segregation population or self-crossed descendants of early segregation generations, said segregation population obtained by hybridizing pairs of parental plants with different desired characteristics to produce population F1, and then hybridizing pairs of F1 one more time. The male parents are homozygous breeding lines, varieties, F1, heterozygous plants in the segregation generations or individual plants produced in the same manner as for the female group. Crop populations and colony varieties of crops display characteristics of consistency, stability, specificity, disease resistance, high yield, and high adaptability while permitting farmers to reserve seeds properly and companies to exploit pedigreed seed on a large scale.

The invention discloses a cultivation method for increasing the yield of Gaza sugar oranges. The method comprises the steps of variety selection, land management, field planting, plant management and disease and pest prevention and cure. According to plant management, plant growth modifier liquid added with water is sprayed before the flower bud differentiation period and during the flower falling period and the fruit expansion period, differentiation of flower buds of the Gaza sugar oranges is promoted during the flower bud differentiation period, flower forming genes are induced, the number of female flowers is effectively increased, stigmas and male flowers are protected, repression is removed, self-pollination or cross-pollination and fertilization are promoted, proline synthesis in crop bodies can be improved, protein denaturation caused by plant cells under the low-temperature and high-temperature states can be avoided, the flowers setting the Gaza sugar oranges are effectively protected from cold injury, and can set fruits normally, and the fruits can be effectively prevented from being scorched by the sun, cracking or shrinking; fruit expansion is accelerated, the fruit shape is lengthened, the effect is remarkable, and the yield of the Gaza sugar oranges is increased.

The invention provides a method for inducing male flowers for seed reservation in the middle and late growing period of female strains of cucumbers, which is used in the breeding field of modern agriculture. Axillae parts where have flower bud primordium germinates on the main tendrils of the female strains of the cucumbers in the middle and later growing period are selected, the axillae parts are sprayed with silver nitrate to induce the germination of male flowers which are used for seed reservation after artificial pollination. The method of the invention relieves the restriction on pollination and fertilization on the high nodes of the female strains of the cucumbers for lacking of male flowers, and solves the problem that seed reservation is hard to realize through self pollination in the middle and later growing period of the female strains of the cucumbers.

The present invention discloses a method of large scale cultivating common wheat near isogenic line. Based on the rule that the self pollination crop has gradual selfing gene isozygoty, the target subunit character of each generation during the gene isozygoty process is tracked and detected by means of biological technology. Through 7-8 generations, the agronomic character of the strain become stable, and two isozygoty types as the paired near isogenic lines of target gene are selfing selected. By means of the said method, several pairs of near isogenic lines may be cultivated inside the same combination, and this result in saving in manpower and material, is favorable to target subunit character analysis and has raised accuracy of statistic and analysis results.

This invention describes a new method to generate hybrid seed in any crop plant while also reducing contamination from out-crossings and self-pollination. In contrast to conventional seed production methods, the method of the invention is not dependent on the use of any form of male sterility in the female parent plants, nor the use of isolation distances from unrelated and undesirable plants of the same species, nor the use of male parent plants in proximity to the targeted females. The process involves the delivery of pollen of the male parent at will, as available either in a preserved pollen bank, or using real-time collection from male plants as they become available. Desired pollen is delivered to fertile females during the period when viable pollen from the females and locally proximal unrelated plants is not being released. The delivered male pollen is in such amounts and fortuitously timed that it preferentially pollinates the females and produces relatively pure hybrid seed at levels much higher than if one used current hybrid production practices and did not utilize male sterility practices or prescribed isolation distances. Such fortuitous timing may involve the intentional application of pollen to females a day or two prior to female parent pollen becoming viable, and / or several consecutive mornings prior to female parent pollen or other proximal plant pollen beginning to shed each day.

The invention relates to a high-yield and multi-resistant method of hybrid seed production for corn variety, belonging to the field of taking advantages of corn hybrid seeds. The method comprises seed selection of inbred lines 216 and 86 by using inbred line 216 as female parent and inbred line 86 as male parent or by using inbred line 86 as female parent and inbred line 216 as male parent to prepare hybrid seed Bidan-13 through multi-generations self-pollination purification, and provides corresponding seed production technique and high-yield culture technique. The prepared Bidan-13 has the characteristics of high yield, good quality, multi-resistance, broad adaptability, and the like; with the average yield per mu of 585.12kg, the corn is averagely increased by 15.37% compared with Xinghuangdan-892, and is an ideal variety for current corn production.

The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology. The molecular marker method: getting F2 filial generation by self-pollination of hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; describing the gene type of each strain of the recombinant inbred lines; constructing genetic linkage map for molecular marker; testing the downy mildew resistance of each strain of the recombinant inbred lines; and composite interval mapping for interval possible located in QTL. The application method: hybridizing S94 and derive verities thereof with other cucumbers and multiplying to F2 generation; separating genome DNA of single cucumber plant, and testing whether each cucumber plant has molecular marker method linked with major QTL for cucumber downy mildew resistance. The invention develops an efficient, rapid breeding technology, selects breeding variety with conventional downy mildew, reduces workload of breeder, and improves precise of selection and breeding efficiency.

The invention discloses a seed production method for producing high-purity hybrid rape seeds by using a double-pollination control system, which combines a self-incompatible line with a chemical male killing technique, uses the self-incompatible line as a female parent of the hybrid seeds to perform inter-planting with a male parent restoration line. A chemical male gametocide is sprayed from the time before blossom to the time of first blossom of the female parent, so the probability of self-pollination of the female parent can be significantly reduced. When the female parent is hybridized with the male parent restoration line, the high-purity hybrid rape seeds can be produced.

The invention relates to a method for promoting self-blossom and fruit bearing of bonsai taxus madia. The technical scheme includes the following steps of: a, selection and cultivation of potting rootstocks; b, treatment of the potting rootstocks; c, selection and treatment of scions; and d, grafting operation of the scions. The invention provides the method for transforming diecious taxus madia into monoecious taxus madia under the condition of bonsai cultivation, the problem that in the prior art, the dioecious bonsai taxus madia cannot complete self-pollination, blooming and fruit bearing is solved, and the economical practicability and ornamental value of the bonsai taxus madia; compared with the prior art in which blooming and fruit bearing of a female plant of taxus madia can be achieved through a method of artificial pollination of the dioecious bonsai taxus madia, no operation of artificial pollination is needed in the method provided by the invention, and under the condition of natural pollination, the female plant of taxus madia can blossom and yield fruit, so that the labor cost is reduced, and the work efficiency is improved.

The invention relates to a breeding method for fresh-eating red waxy hybrid maize belonging to the hybrid maize breeding field, comprising the procedures as follows: black pearl waxy corn with perfect plasmid and modena grain chosen as male parent and Darfur waxy corn with perfect plasmid and silvery white grain chosen as female parent are hybridized to establish an underlying group; recurrent selection is carried through in the underlying group in the aspects of red grain, waxy property, sweet taste, single plant production capacity, the ability of anti tan disease and lodging resistance, and repeated anisotropic hybridization and self-pollination are carried through to establish a hybridization group; the self-fertilization of parent strain and retrogression choiceness of the hybridization group are completed, vermeil hard grain is produced after 4-6 generations. The breeding method of the invention can breed the fresh-eating and eatable red waxy corn with high officinal value, good mouthfeel, high contents of natural haematochrome and rich nutrition which can be used in industry and as feed and has the advantages of strong ability to resist disease, drought resistance, flood resistance, high yield and fertility.

The invention relates to the breeding of cucumbers, and especially to a new cucumber plant with a valuable new characteristic. This new characteristic leads to an improvement in the growth properties and so to a simplification and improvement of cucumber cultivation. Seeds according to the invention and methods for introducing the new characteristic into other cucumber plants are also covered. Self-pollination and cross-pollination of the plants according to the invention are described, as well as the production of doubled haploids from these plants.

The invention discloses an acquisition method of a molecular marker closely linked with salt-tolerant main effect QTL (Quantitative Trait Loci) at the tomato seedling stage, comprising the following steps of: positioning by the salt-tolerant main effect QTL at the tomato seedling stage; selecting M82 at the tomato seedling state and a salt-tolerant introgressed line IL7-5 to hybridize to obtain ahybrid F1 for self-pollination to obtain a group F2; screening a polymorphism molecular marker between a salt-tolerant sub-introgressed line 7-5-5 and a salt-sensitive variety M82 by the salt stress processing; screening the F2 group by using the screened side wing marker on the IL7-5-5 and establishing a recombinant F2 group and genetic analysis; and determining the molecular marker closely linked with salt-tolerant main effect QTL at the tomato seedling stage by the salt-tolerant survival rate and the marker detection in a genetic linkage map. The invention is not influenced by environment conditions, can be used for screening progeny and derivative strains of the salt-tolerant materials at the specific seedling stage at early generation, saves the cost and improves the breeding and selecting efficiency.

The invention discloses a robot path planning method based on a chaotic backward flower pollination algorithm. Key nodes are used for expressing a path of a robot; and the key nodes of the path of the robot are optimized by using a chaotic backward flower pollination algorithm. According to the chaotic backward flower pollination algorithm, a backward learning strategy having a Lorenz chaotic weight is introduced; and a Lorenz chaotic sequence is fused into a crossed pollination operation and a self-pollination operation to enhance the global search ability of the flower pollination algorithm and improve the solution quality. Therefore, the robot path planning efficiency is improved.

The invention discloses a method for quickly potting pear trees in the four seasons in the cold areas. The method comprises the following steps: (1) selecting places: the greenhouses and grounds are mainly selected; (2) selecting pots: both the height and the diameter of the pots are 25cm; (3) selecting varieties: the self-pollinated varieties with more pollens are selected; (4) preparing nutritive soil: the farm manures, fertilizers and biological bacterial manures are selected to prepare the nutritive soil; (5) carrying out planting: the soil is filled till the position 5cm from the root during planting; (6) carrying out grafting: carrying out selective multi-variety grafting or fixed grafting (various fruits can be grafted on one tree); (7) carrying out pruning at the right time; (8) bending the branches at the right time; (9) carrying out shaping: the branches and trunks of the pear trees are used for making various patterns and shapes of Chinese characters (such as xi, fu and the like) through designing, pruning and branch bending; (10) bearing fruits by combining chemical control with pruning or combining pruning with chemical control. The method has the following beneficial effects: the time from planting to appearing on the market is shortened to one year by adopting the method; and short cycle, quick effect and high yield are realized.

A method for breeding the new pear germplasm with self pollination features that the earier ripe pear 'Bali' whose S gene type is S2S5 or 'Changshilang' whose S gene type is S2S3, and the pear 'Aogaershishiji' with self pollination are used to hybridize to obtain two excellent lines '98-19-117' and '99-6-39', whose self pollination is more than 70%.

The invention discloses a short-term cultivation method of a peach tree in a pot in the North, which comprises the following steps of: (1) the selection of places: selecting the places which are main a hothouse and the ground; (2) the selection of the pot: selecting the pot with the height of 25cm and the diameter of 25cm; (3) the selection of breed: selecting a breed characterized in much pollen and self-pollination; (4) the preparation of nutrition soil: selecting farmyard manure, fertilizers and biological bacterial fertilizers to prepare the nutrition soil; (5) planting: burying the soil 5 centimeters above the root part of the peach tree during the planting; (6) grafting: selectively or unchangeably grafting a plurality of breeds (one tree can produce a plurality of fruit); (7) trimming: trimming branches at proper time; (8) the bending of shoots: bending the shoots at proper time; (9) shaping: shaping the branches and the trunk of the peach tree into various patterns and characters (such as a Chinese character 'xi (happiness)', a Chinese character 'fu (fortune)', a pattern and the like) through a design, the trimming and the bending of the branches; and (10) the production of fruit: producing the fruit through the matching of the combination of chemical control and the trimming or the combination of the trimming and the chemical control. Through the above technology, the period from the planting to the marketing is shortened to one year; therefore, short period, high efficiency and high yield are realized.

The invention relates to a culturing method for new species of large cherry rock candy cherry, relating to the production of large cherry, solving the problems of low large cherry sweetness, low hardness, no storage and transport-resistance and no fruit cracking resistance. The method comprises the following steps: selecting the Chinese cherry from Qinling Mountain for seeding, which is the large cherry category cultured in Shanxi area with high sweetness, high hardness, storage and transport-resistance and light-resistance fruit cracking; finding a well growing and property individual plant; spreading after grafting into the large cherry; selecting the cultured branch as cion; grafting by green branches; and culturing in Shanxi area. The culturing method has the characteristics of good quality, early fruit, abundant production, half-opened tree posture, green leaves, exuberant tree, good early fruit property, large fruit size, red color, good hardness, storage and transport-resistance, sweet taste, core separation, good taste and short fruit branch; the weight of each fruit is about 9-12 g; the ripening time is slightly later than the red cherry; more than 90% branches are formed with fruits after the full bearing period; the culturing method has late frost-resistance, self-pollination and light fruit cracking-resistance; the comprehensive quality is better than the current red cherry planted in bulk; and the rock candy cherry is the category with good developing foreground.

The invention discloses a quick cultivation method of a fruit tree in a pot at all seasons in the North, which comprises the following steps of: (1) the selection of places: selecting the places which are main a hothouse and the ground; (2) the selection of the pot: selecting the pot with the height of 25cm and the diameter of 25cm; (3) the selection of breed: selecting a breed characterized in much pollen and self-pollination; (4) the preparation of nutrition soil: selecting farmyard manure, fertilizers and biological bacterial fertilizers to prepare the nutrition soil; (5) planting: burying the soil 5 centimeters above the root part of the fruit tree during the planting; (6) grafting: selectively or unchangeably grafting a plurality of breeds (one tree can produce a plurality of fruit); (7) trimming: trimming branches at proper time; (8) the bending of shoots: bending the shoots at proper time; (9) shaping: shaping the branches and the trunk of the fruit tree into various patterns and characters (such as a Chinese character 'xi (happiness)', a Chinese character 'fu (fortune)', a pattern and the like) through a design, the trimming and the bending of the branches; and (10) the production of fruit: producing the fruit through the matching of the combination of chemical control and the trimming or the combination of the trimming and the chemical control. Through the above technology, the period from the planting to the marketing is shortened to one year; therefore, short period, high efficiency and high yield are realized.

This invention provides a novel means of making maize hybrids and inbreds with expanded ear tip phenotypes for promoting increased seed yield from recessive genetic determinants. This is achieved by making standard inbred×hybrid as well as inbred×inbred crosses followed by selection in the subsequent self-pollinated generations from these plants for expanded ear tip phenotype characteristics to produce new parent inbred lines. Next, the resulting inbreds, having a different pedigree but possessing the recessive expanded ear tip genetic determinants, are crossed to cause the expanded ear tip trait to express as a homozygous recessive trait in an otherwise primarily heterozygous hybrid.

The invention discloses a simple isolation method for eggplant flowering stage pollination, which belongs to the technical field of plant hybridization. The method of the present invention comprises the following steps: (1) selecting a cotton thread with a diameter of 0.7-1.0mm and a length of 8-10cm, and beating the cotton thread into a live button that can be pulled back and forth; (3) collect the male parent's anthers, and dry them for later use; (4) take off the cotton thread loops, pollinate, and tie the cotton thread loops on the top of the female parent's flower buds again. The invention makes the eggplant hybridized flowers have better ventilation and light transmission, avoids the phenomenon of fruit drop and rotten fruit, and greatly improves the fruit setting rate of the hybridized flowers. At the same time, the cotton thread is easy to operate and the cost is low, which can effectively improve the time of the producer and improve the economic benefits.

This invention describes a new method to increase grain yields in any crop plant by modifying pollination to effect an increase in grain yield, a change in grain content or characteristics, a decrease in contamination, or a combination of these attributes. The process involves the intentional delivery of pollen of the male plant at will, as available either in a preserved pollen bank, or real-time collection from male plants as they become available, in a growth chamber for example. Desired pollen is delivered to fertile females. The delivered pollenM is in such amounts and fortuitously timed that it preferentially pollinates the females and optionally, avoids or enhances self-pollination or pollination from neighboring plants. The intentional delivery of genetically different pollen will result in increased heterosis and accompanying grain yield increases resulting from increased grain size and the potential to influence grain content and constituents. The invention also permits real-time agronomic decision making in order to maximize grain yield by overcoming biotic and abiotic challenges in the growing season which may or may not have been anticipated. The intentional delivery of self- or sib-pollen results in a decrease in contamination from undesirable outcrossing.

The invention discloses a selfing method of a herbaceous-flower corn poppy, comprising the following steps of: when a first poppyhead of a single plant of a corn poppy blooms on the same day, selecting a single-seed color single plant in a parental propagating field, marking colors by inserting a card near each plant, and picking off the poppyhead at the same time, wherein the bud top of 1 / 5 of first-fourth first-branching poppyheads of each single plant is stapled by a laminater, a flower blooms after stapled by stapling nails on the second day and the third day, the bottom of a sepal cracks, the top of the flower is retained at the top end of a petal, and stamens and pistils are completely coated by the petals to realize self-fruition; ensuring the complete self-fruition of each color by picking off unstapled flowers; selecting the bud with the length of 25-35 mm according to varieties and blooming characteristics of the corn poppy every two days and stapling the bud of 1 / 5 by the laminater; and picking off the unstapled flowers at the same time. The selfing method is adopted to prevent flowers from blooming naturally in a blooming period, and can effectively block the introduction of external pollen and realize self-pollination fruition.