Cucumber downy mildew resistance main effect QTL linkage molecule labeling method and applying method

Abstract

The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology. The molecular marker method: getting F2 filial generation by self-pollination of hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; describing the gene type of each strain of the recombinant inbred lines; constructing genetic linkage map for molecular marker; testing the downy mildew resistance of each strain of the recombinant inbred lines; and composite interval mapping for interval possible located in QTL. The application method: hybridizing S94 and derive verities thereof with other cucumbers and multiplying to F2 generation; separating genome DNA of single cucumber plant, and testing whether each cucumber plant has molecular marker method linked with major QTL for cucumber downy mildew resistance. The invention develops an efficient, rapid breeding technology, selects breeding variety with conventional downy mildew, reduces workload of breeder, and improves precise of selection and breeding efficiency.

Description

technical field [0001] The invention relates to a marker method in the field of molecular biology technology, in particular to a molecular marker method and an application method for the main effect QTL linkage of cucumber downy mildew resistance. Background technique [0002] Downy mildew (downy mildew) is one of the important diseases of cucumber (Cucumis sativus L.) producing areas all over the world. In my country, downy mildew is listed as the three major diseases of cucumber along with downy mildew and Fusarium wilt. Cucumber downy mildew is caused by Pseudoperonospora cubensis (Berk. & Curt.) Rostov.], which belongs to the subphylum Flagellate, Peronosporales, and the genus Pseudoperonospora. It mainly harms the leaves of cucumbers, and can be diseased at the seedling stage and the adult plant stage. Under suitable conditions, its disease develops rapidly, and it will spread to the whole field in a few days, causing the leaves to turn yellow and die, and the yield wi...

AI Technical Summary

Problems solved by technology

In conventional breeding practice, it is very difficult to select materials resistant to downy mildew: on the one hand, the breeding cycle is long; on the other hand, the occurrence of downy mildew is affected by many factors, such as: temperature, humidity and pathogen race, etc. , the process of identifying resistant materials is not easily controlled

These are the important reasons for the slow progress of cucumber downy mildew breeding

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