32results about How to "Speed ​​up the selection process" patented technology

The present invention relates to a molecular marking method for pig litter size. The method comprises the steps of pig genome DNA extraction, exon 6 primer design of sex hormone binding globulin (SHBG) genes, amplification in vitro and genotype detection. The present invention first discovers pig SHBG genes, discovers the obvious relationship between the polymorphism of exon 6 and pig litter size, and develops a restriction fragment enzyme digestion polymorphism detection method for detecting exon 6 mutational sites. The method can be used for the auxiliary selection of litter size during pig breeding, can realize the early selection of parent pigs and even can accurately select parent pigs when the pigs are born. Generation interval is largely shortened, and selection progress is accelerated. The method has the advantages of simple operation, low condition requirements in polymerase chain reaction processes, short amplification fragment length (217bp), easy amplification, high amplification efficiency and accurate genotype judgement.

The invention relates to a method for breeding lean-type Chinese Huai pigs in a multi-gene pyramiding manner based on growth traits thereof. The method comprises the following operating steps: 1, carrying out DNA (deoxyribonucleic acid) extraction on the genome of the pig; 2, designing primers, more particularly, designing the PCR (polymerase chain reaction) amplification primers according to the gene sequences of insulin-like growth factor-I (IGF-I), pituitary specific transcription factor-I (PIT-I), liver X receptor alpha (LXR alpha) and melanocortin-4 receptor (MC4R); 3, carrying out the PCR; 4, carrying out restriction fragment length polymorphism (RFLP); and 5, carrying out the correlation analysis on the polymorphism and growth traits of genes. According to the analysis, the method can determine that the single growth rate of the pyramided gene with the gene type thereof being AADDGGFF is the highest one; the method can prevent the genes of other offsprings from being isolated after the selective reservation for breeding, thus achieving the optimal effect of the growth traits of the offsprings on the four gene types; and the method can stabilize the inheritance, particularly lead the genes of the growth traits to become homozygous within one generation, thus accelerating the cultivation of the lean-type Chinese Huai pigs.

The invention relates to a hydraulic stability analysis method for a pump station, in particular to the pump station for multi-mode operation and belongs to the technical field of hydraulic engineering pump stations. Tangential speeds of various points of an inlet section of an outlet passage are obtained on the basis of a three-dimensional steady numerical calculation result of the whole passage of a pump device; a calculation formula of a momentum parameter SM is built; and the tangential speed of the inlet section of the outlet passage is determined by the calculation formula of the momentum parameter SM. The method is advanced and scientific; by the method, the hydraulic stability of the pump device in different schemes can be comprehensively compared; the oneness that only the efficiency of the pump device is adopted as an evaluation index is avoided; the problems of long elapsed time and high requirements on hardware equipment when the whole passage of the pump device is determined by a three-dimensional unsteady numerical calculation method are solved; quick analysis on the actual hydraulic stability of the pump station is facilitated; the time period of comparing pump station schemes is shortened; the target of quickening the engineering scheme selection progress of the pump station is achieved; and a reliable method is also provided for optimization of the pump device in different schemes.

The invention discloses a molecular marking method of a heat-proof major QTL lotus qHTF-1 in a rice heading and flowering period. According to the method, a primer pair of a molecular marker RM23 or one pair or two pairs of primers in the primer pairs of a molecular marker RM562 is applied to perform PCR amplification on the DNA of a to-be-identified rice material; if a DNA fragment of corresponding size is amplified, heat-proof major QTL qHTF-1 exists. According to the method, by adopting 2 SSR markers in close linkage with a heat-proof major QTL qHTF-1 in the heading and flowering period located on the Teqing 1-st chromosome of a heat-proof rice variety, the high-temperature tolerance of the rice material is predicted, the breeding of a new variety of heat-proof and stress-resistant riceis facilitated, and the selection progress of a heat-proof rice variety is accelerated.

The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology. The molecular marker method: getting F2 filial generation by self-pollination of hybrid F1; acquiring F7 generation recombinant inbred lines by filial generation single seed descent method; describing the gene type of each strain of the recombinant inbred lines; constructing genetic linkage map for molecular marker; testing the downy mildew resistance of each strain of the recombinant inbred lines; and composite interval mapping for interval possible located in QTL. The application method: hybridizing S94 and derive verities thereof with other cucumbers and multiplying to F2 generation; separating genome DNA of single cucumber plant, and testing whether each cucumber plant has molecular marker method linked with major QTL for cucumber downy mildew resistance. The invention develops an efficient, rapid breeding technology, selects breeding variety with conventional downy mildew, reduces workload of breeder, and improves precise of selection and breeding efficiency.

The invention relates to a molecular marker tightly linked with a major QTL (Quantitative Trait Loci) of the cotton seed oil content of upland cotton and application thereof. The molecular marker tightly linked with the major QTL of the cotton seed oil content of upland cotton is TMB1216. The molecular marker TMB1216 can be applied to the genotype detection of an upland cotton variety or strain to judge the oil content of the variety or strain. In the invention, the molecular marker TMB1216 is used for assisting in selecting, so that high accuracy is achieved, the selecting speed is increased, the breeding workload is lowered greatly simultaneously, the breeding efficiency is increased, and the defects and disadvantages of long period, high subsequent screening workload, low verification efficiency of the variety or strain and the like existing in the conventional breeding are overcome.

The invention relates to a molecular marker tightly linked with a wheat yellow mosaic resistant major quantitative trait locus (QTL). The molecular marker is characterized in that: polymerase chain reaction (PCR) amplification is performed on deoxyribonucleic acid (DNA) of a wheat variety CI12633 by using PCR primers, namely, Wmc327 and Gwm154; an amplification product is subjected to electrophoretic separation on 12 percent polyacrylamide gel so as to obtain molecular markers, namely, Xwmc327-180 and Xgwm154-105 tightly linked with the wheat yellow mosaic resistant major QTL; the sizes of the Xwmc327-180 and the Xgwm154-105 are 180bp and 105bp respectively; and the molecular markers are used for detecting the genotype of a derivative variety or strain of the wheat variety CI12633 so as to judge whether the derivative variety or strain can resist wheat yellow mosaic or not.

The invention relates to the field of molecular genetics, discloses a molecular marker of a pear PpAIV2 gene locus and further discloses a screening method of the molecular marker of the pear PpAIV2 gene locus. A genome simple sequence repeat (SSR) locus adjacent to pear soluble acid invertase gene PpAIV2 is predicated and a locus specific primer is designed; one SSR allelic variation locus which is tightly interlocked with the PpAIV2 is obtained through amplification in germplasms with different genetic backgrounds and F1-generation population genomes; the SSR allelic variation locus is named as P.zaas-1. The pear soluble acid invertase gene PpAIV2 is a key gene for accumulating pear fruit sucrose; the P.zaas-1 has potential identification and screening effects on materials with remarkable differences on accumulation of the fruit sucrose.

The invention discloses a molecular marking method of a chalkiness-ratio major-effect QTL (Quantitative Trait Locus) in rice of paddy rice. The method comprises the following steps of carrying out PCR (Polymerase Chain Reaction) amplification on the DNA (Deoxyribonucleic Acid) of a to-be-authenticated paddy rice material by utilizing one pair or two pairs of a primer pair of a molecular marker RM20660 or a primer pair of a molecular marker RM7412, carrying out electrophoretic detection on an amplified product, and if a DNA fragment in a corresponding size is amplified, indicating that a low-chalkiness-ratio major-effect QTL qCD6 exists. According to the method, through applying two sieved SSR (Simple Sequence Repeat) markers which are closely interlocked with the chalkiness-ratio major-effect QTL qCD6, which is located on a sixth chromosome of a low-chalkiness-ratio paddy rice variety Lemnot, of the rice, the chalkiness ratio of the paddy rice material is predicted; the selective breeding of a high-quality low-chalkiness-ratio paddy rice new variety is facilitated; the selection progress of a high-quality low-chalkiness-ratio paddy rice variety is quickened.

The invention aims to provide the KASP molecular marker for detecting the color of the waxberry fruit and the application, the SNP site of the molecular marker is located at the 4th, 691st and 249th basic groups of the waxberry chromosome 6, and a KASP primer combination is designed by utilizing the difference of the single basic groups and can be used for quickly and accurately identifying the genetic typing of the site of the waxberry so as to predict the color of the waxberry fruit. The method can be used for selecting germplasm materials with required fruit colors, greatly quickens the selection progress of the myrica rubra germplasm materials, is simple, convenient, efficient and low in cost, and has a good application prospect in the aspects of myrica rubra fruit color identification and auxiliary breeding.

The invention relates to the technical field of Internet, and particularly discloses a Raft-based hot standby multi-master method. Comprising the following steps: step S001, after finishing Leader election, selecting a plurality of standby Leader nodes from a node cluster; step S002, identifying the selected standby Leader node, and marking the selected standby Leader node as a'standby node '; step S003, a plurality of standby node clusters are set as standby node clusters, and standby nodes in the standby node clusters are numbered in sequence in an incremental order; step S004, the selected standby Leader node is forced to be synchronized with the log; and step S005, when the Leader is crashed, the standby nodes vote according to the number sequence from small to large as the priority, and the problem that the master selection time is too long when the traditional Leader is crashed is solved.

The invention discloses a tissue culture method for Pruns avium L intraspecific hybridization F1-generation grown-up seedlings. The tissue culture method comprises hybridization seed production and seed collection. A collected seed comprises the following steps of in sequence: S1: carrying out seed hull-breaking processing and seed embryo dipping; S2: carrying out seed embryo disinfection and inner seed coat removal; S3: carrying out inoculation and culture; S4: checking an embryo pollution situation and secondary disinfection; S5: carrying out browning processing and timely transferring; andS6: carrying out acclimatization and transplantation. The tissue culture method only uses one MS solid culture medium which contains an exogenous growth promoting additive, time for taking roots fromgermination is short, the tissue culture method is simple and convenient to operate, hybridization F1-generation seedling growth time is greatly shortened, a whole seed breeding process is shortened,and Pruns avium L breeding efficiency is improved.

The invention provides a three-primer molecular marking method for detecting an imidazolone herbicide resisting gene of cabbage type rape and belongs to the field of plant molecular breeding. Primer pairs of AP15F / AP18R and AP15F / AP19R are added into two polymerase chain reaction (PCR) reaction systems respectively to subject cabbage type rape deoxyribonucleic acid (DNA) to amplification, and homozygote free of resistance gene BnALS1R, homozygote containing resistance gene BnALS1R and heterozygote containing resistance gene BnALS1R are distinguished. By means of the method, whether rapeseed germplasm resources or breeding population of the rape contain the imidazolone herbicide resisting gene BnALS1R or not can be rapidly and accurately detected, whether the contained herbicide gene is the homozygote or the heterozygote can be accurately distinguished, the selection efficiency of the resistance gene BnALS1R can be improved, and the breeding process of imidazolone herbicide resisting rape breeds can be accelerated.

The invention discloses a molecular marker for an eye muscle pH value-related gene SVEP1 within 24 hours after slaughter of pigs and application of the molecular marker. The molecule of the eye muscle pH value-related gene SVEP1 is marked as a group of molecular markers, comprising a molecular marker SVEP1-PHU1 and a molecular marker SVEP1-PHU2, respectively, wherein the nucleotide sequence of SVEP1-PHU1 has an SNP site A / G at 641bp; the nucleotide sequence of SVEP1-PHU2 has an SNP site C / G at 94bp. According to the invention, the group of molecular markers can be combined to rapidly predict the difference between eye muscle pH values within 24 hours after slaughter of different genotype pigs; meanwhile, the molecular marker can be used as a reliable marker of eye muscle pH value within 24 hours after slaughter in pig breeding work, effectively shortens breeding period, accelerates selection process, and promotes cultivation of new varieties.

The invention relates to the field of molecular genetics, discloses a molecular marker of a pear PpAIV2 gene locus and further discloses a screening method of the molecular marker of the pear PpAIV2 gene locus. A genome simple sequence repeat (SSR) locus adjacent to pear soluble acid invertase gene PpAIV2 is predicated and a locus specific primer is designed; one SSR allelic variation locus which is tightly interlocked with the PpAIV2 is obtained through amplification in germplasms with different genetic backgrounds and F1-generation population genomes; the SSR allelic variation locus is named as P.zaas-1. The pear soluble acid invertase gene PpAIV2 is a key gene for accumulating pear fruit sucrose; the P.zaas-1 has potential identification and screening effects on materials with remarkable differences on accumulation of the fruit sucrose.

The invention discloses a KASP marker primer for identifying imidazolinone herbicide-resistant rape, a kit and application of the KASP marker primer and the kit, and relates to the technical field of molecular genetic breeding of crops. The KASP marker primer group disclosed by the invention comprises a KASP marker primer group BN-PM1.1 and a KASP marker primer group BN-PM1.2 which are used for detecting an AHSH1 gene, and a KASP marker primer group BN-PM2.1 and a KASP marker primer group BN-PM2.2 which are used for detecting an AHSH3 gene. The KASP marker primer group is high in specificity, good in quality, high in sensitivity and high in resolution, and can accurately, quickly, efficiently and specifically identify the variation sites of PM1 and PM2 in the AHAS1 gene and the AHAS3 gene of the rape.

The invention discloses a molecular marker for an eye muscle pH value-related gene SVEP1 within 24 hours after slaughter of pigs and application of the molecular marker. The molecule of the eye muscle pH value-related gene SVEP1 is marked as a group of molecular markers, comprising a molecular marker SVEP1-PHU1 and a molecular marker SVEP1-PHU2, respectively, wherein the nucleotide sequence of SVEP1-PHU1 has an SNP site A / G at 641bp; the nucleotide sequence of SVEP1-PHU2 has an SNP site C / G at 94bp. According to the invention, the group of molecular markers can be combined to rapidly predict the difference between eye muscle pH values within 24 hours after slaughter of different genotype pigs; meanwhile, the molecular marker can be used as a reliable marker of eye muscle pH value within 24 hours after slaughter in pig breeding work, effectively shortens breeding period, accelerates selection process, and promotes cultivation of new varieties.

The invention discloses primers for detecting an anti-sulfonylurea herbicide gene BnALS3R of cabbage type rape and the application of the primers, and belongs to the field of molecular breeding. A method for detecting the anti-sulfonylurea herbicide gene BnALS3R of the cabbage type rape by use of the primers SEQ ID NO.1 and SEQ ID NO.2 comprises the following steps: taking the total DNA of the cabbage type rape as a template and adding a primer for PCR amplification; performing restriction enzyme BsrDI digestion on the PCR product; differentiating homozygous rape containing the resistance gene BnALS3R, hybrid rape containing the resistance gene BnALS3R, and homozygous rape not containing the resistance gene BnALS3R by use of the enzyme digestion product. According to the primers, whether rape planets contain the resistance gene BnALS3R can be detected quickly and accurately, and whether the contained anti-herbicide gene is a homozygous type or a hybrid type also can be detected accurately, and therefore, the breeding objective of breeding a new anti-herbicide rape variety can be achieved quickly and effectively.

The invention relates to the field of molecular genetics and discloses a molecular marker of a peel full-brown trait gene locus of Chinese 'Qingxiang'-variety pears and a screening method of the molecular marker of the peel full-brown trait gene locus of the Chinese 'Qingxiang'-variety pears. A peel full-brown trait and a genotype of each F1 individual plant obtained by hybridization of Chinese 'Qingxiang'-variety pears and Chinese 'Guancui'-variety pears are subjected to genetic linkage analysis to obtain the molecular marker of the peel full-brown trait gene locus Rus.zaas-8 of the Chinese 'Qingxiang'-variety pears. Whether the gene locus is contained in the 'Qingxiang' variety or other derived varieties (strains) or not is detected by means of the molecular marker of the peel full-brown trait gene locus, peel colors of fruits can be predicated, and accordingly selecting efficiency of fruit peel colors of Chinese pears can be greatly improved.

The invention relates to a molecular marker tightly linked with a wheat yellow mosaic resistant major quantitative trait locus (QTL). The molecular marker is characterized in that: polymerase chain reaction (PCR) amplification is performed on deoxyribonucleic acid (DNA) of a wheat variety CI12633 by using PCR primers, namely, Wmc327 and Gwm154; an amplification product is subjected to electrophoretic separation on 12 percent polyacrylamide gel so as to obtain molecular markers, namely, Xwmc327-180 and Xgwm154-105 tightly linked with the wheat yellow mosaic resistant major QTL; the sizes of the Xwmc327-180 and the Xgwm154-105 are 180bp and 105bp respectively; and the molecular markers are used for detecting the genotype of a derivative variety or strain of the wheat variety CI12633 so as to judge whether the derivative variety or strain can resist wheat yellow mosaic or not.

The invention relates to an analysis method for the hydraulic stability of a pumping station, especially for a pumping station operating under multiple working conditions, and belongs to the technical field of hydraulic engineering pumping stations. Based on the three-dimensional constant value calculation results of the whole flow channel of the pump device, the tangential velocity of each point of the inlet section of the outlet channel is obtained; the calculation formula of the momentum parameter SM is constructed; the tangential velocity of the inlet section of the outlet channel is calculated by using the calculation formula of the momentum parameter SM Solve. The method of the present invention is advanced and scientific, and the method can be used to comprehensively compare the hydraulic stability of pump devices of different schemes, avoid the singleness of using only the efficiency of the pump device as an evaluation index, and solve the problem of using three-dimensional unsteady numerical calculations to solve the calculation of the entire channel of the pump device The problems of long time consumption and high requirements for hardware equipment are conducive to the rapid analysis of the hydraulic stability of the actual pumping station, saving the time period of pumping station scheme comparison, achieving the purpose of speeding up the selection of pumping station engineering schemes, and also providing support for different schemes of pumping devices. Preferably a reliable method is provided.

The invention discloses a tissue culture method for rapid growth of sweet cherry intraspecific hybrid F1 generation seedlings, which includes hybrid seed production and seed collection; the collected seeds are sequentially subjected to the following steps: S1, seed breaking treatment and seed embryo soaking; S2 1. Embryo disinfection and inner testa removal; S3, inoculation and cultivation; S4, inspection of embryo contamination and secondary disinfection; S5, browning treatment and timely transfer; S6, seedling hardening and transplanting. The present invention only uses a MS solid medium containing exogenous growth-promoting additives, the time required from germination to rooting is relatively short, the operation is simple and convenient, and the time for seedling formation of the hybrid F1 generation is greatly shortened, the entire breeding process is shortened, and sweetness is improved. Cherry breeding efficiency.

The invention discloses a pig 5-aminolevulinic acid synthetase 1 gene as a genetic marker of pig litter size traits and an application thereof. The nucleotide sequence of the genetic marker is shown as SEQ ID NO.1, and R at a 26-site base in the sequence is T or C. The mutation causes polymorphism of a 5'flanking region of the porcine 5-aminolevulinic acid synthase 1 gene. According to the invention, the SNP locus of the 5'flanking region of the pig ALAS1 gene is discovered for the first time; the relationship between the SNP locus and the pig litter size traits is analyzed; the molecular marker related to the pig litter size is found; and a rapid detection method is established.

The present invention relates to a molecular marking method for pig litter size. The method comprises the steps of pig genome DNA extraction, exon 6 primer design of sex hormone binding globulin (SHBG) genes, amplification in vitro and genotype detection. The present invention first discovers pig SHBG genes, discovers the obvious relationship between the polymorphism of exon 6 and pig litter size, and develops a restriction fragment enzyme digestion polymorphism detection method for detecting exon 6 mutational sites. The method can be used for the auxiliary selection of litter size during pigbreeding, can realize the early selection of parent pigs and even can accurately select parent pigs when the pigs are born. Generation interval is largely shortened, and selection progress is accelerated. The method has the advantages of simple operation, low condition requirements in polymerase chain reaction processes, short amplification fragment length (217bp), easy amplification, high amplification efficiency and accurate genotype judgement.