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A caps marker method for distinguishing banana fusarium wilt resistant varieties

A labeling method and molecular labeling technology, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of long screening period, difficult selection of new varieties, high cost of labor and land, etc. Achieve the effect of improving selection efficiency, saving field breeding costs, and improving selection process

Active Publication Date: 2021-01-19
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

The second is the lack of breeding methods, and it is difficult to breed new varieties
The main variety of banana planted is triploid, so it is difficult to adopt cross-breeding in the breeding method, while the banana breeding method based on radiation mutagenesis needs to maintain a large number of screening populations, and the selection of superior plants is mostly based on morphological markers, resulting in the existence of screening in its breeding. The cycle is long, and the cost of labor and land is too high

Method used

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  • A caps marker method for distinguishing banana fusarium wilt resistant varieties
  • A caps marker method for distinguishing banana fusarium wilt resistant varieties
  • A caps marker method for distinguishing banana fusarium wilt resistant varieties

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Embodiment Construction

[0022] Referring to the accompanying drawings, the present invention will be further described in conjunction with specific embodiments, so as to better understand the present invention. If no specific technique or condition is indicated in the examples, it shall be carried out according to the technique or condition described in the literature in this field or according to the product specification. The reagents or instruments used were not indicated by the manufacturer, and they were all commercially available conventional products.

[0023] 1. Genomic DNA extraction

[0024] Randomly select 4 healthy plants of Banana braziliana and Zhongre No. 1 banana respectively, and use the young roll leaves as materials to extract genomic DNA using the polysaccharide and polyphenol plant genomic DNA extraction kit (DP360) of Beijing Tiangen Biochemical Technology Co., Ltd. , 1% agarose gel electrophoresis to detect DNA quality.

[0025] 2. Identification of SNP and InDel mutation sit...

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Abstract

The invention provides a CAPS marking method for distinguishing banana wilt resistant varieties. InDel mutation capable of being used for distinguishing Zhongre No.1 from Brazilian banana is obtainedby sequencing and analyzing the Zhongre No.1 and the Brazilian banana. The mutation causes the difference of the gene sequence on the digestion of a BclI incision enzyme; and therefore, the CAPS marking method for distinguishing banana wilt resistant varieties is designed. The method can directly reflect the resistance of a plant, does not have error identification caused by genetic exchange, caneffectively identify the main cultivated variety Brazilian banana and the disease-resistant variety Zhongre No.1 bred by radiation mutation breeding, and greatly improves the selection efficiency of resistance genes. Moreover, when being used for identification in a seedling stage of bananas, the marking method not only can save field breeding cost, but also can greatly improve the selection process of wilt-resistant banana varieties, and lays a foundation for banana resistance mutation breeding.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a CAPS marking method for distinguishing banana fusarium wilt resistant varieties. Background technique [0002] Cleaved amplified polymorphic sequence (CAPS) is a molecular marker method produced by combining PCR reaction and restriction endonuclease digestion. The basic principle is to design a set of specific PCR primers with the DNA sequence of the known site, and after amplification, use a specific restriction endonuclease to digest the amplified product for restriction fragment length polymorphism. gender analysis. CAPS markers have the characteristics of co-dominance, specificity, simple operation and low cost, and are currently mainly used in the research of plant gene mapping, typing, map-based cloning and identification of varieties and strains. [0003] Banana is an important tropical economic crop with both fruit and food. Bananas are grown in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113C12Q2521/301C12Q2565/125
Inventor 王静毅王卓贾彩红刘菊华徐碧玉金志强张建斌苗红霞
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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