Molecular marking method of heat-proof major QTL lotus qHTF-1 in rice heading and flowering period and application

Abstract

The invention discloses a molecular marking method of a heat-proof major QTL lotus qHTF-1 in a rice heading and flowering period. According to the method, a primer pair of a molecular marker RM23 or one pair or two pairs of primers in the primer pairs of a molecular marker RM562 is applied to perform PCR amplification on the DNA of a to-be-identified rice material; if a DNA fragment of corresponding size is amplified, heat-proof major QTL qHTF-1 exists. According to the method, by adopting 2 SSR markers in close linkage with a heat-proof major QTL qHTF-1 in the heading and flowering period located on the Teqing 1-st chromosome of a heat-proof rice variety, the high-temperature tolerance of the rice material is predicted, the breeding of a new variety of heat-proof and stress-resistant riceis facilitated, and the selection progress of a heat-proof rice variety is accelerated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a molecular marker method for heat-resistant major QTL site qHTF-1, an important stress-resistant trait of rice, and also relates to the application of the molecular marker method in breeding heat-resistant and stress-resistant rice varieties. Background technique [0002] The continuous warming of the global climate has brought a very serious impact on China's rice production. The period when rice is most sensitive to temperature is the heading and flowering period. During this period, if the daily average temperature is higher than 35°C, it is easy to induce sterility of florets, resulting in obstacles to fertilization, resulting in failure to set seeds and reducing yield. During the heading and flowering stage, the high temperature and heat damage caused a significant drop in the seed setting rate and reduced production. For example, in the summer of 2003, the high temp...

AI Technical Summary

Problems solved by technology

The period when rice is most sensitive to temperature is the heading and flowering period. During this period, if the daily average temperature is higher than 35°C, it is easy to induce infertility of florets and cause fertilization obstacles, resulting in failure to set seeds and reduce yield.

During the heading and flowering stage, the high temperature and heat damage caused a significant drop in the seed setting rate and reduced production. For example, in the summer of 2003, the high temperature above 38°C lasted for more than 20 days in some southern rice regions. In 2006, a severe high temperature disaster occurred in the Sichuan-Chongqing region, resulting in a reduction of more than 25% of rice production in that year; Suffering from a wide range of continuous high temperature hazards above 38°C, resulting in a large-scale reduction in rice and other food crops, and a serious drop in seed setting rate of 50%

Images