77results about How to "Shorten the generation interval" patented technology

The invention discloses a crossing breeding and feeding method of wild boars, domestic pigs and hybrid pigs. The wild boars are not fit for feeding and popularization for the reasons that the number of the wild boars is limited, the meat is not fine and the skin is thick, and mouthfeel is worse. The wild boars are oestrous seasonably, produce fewer piglets, and are not easy to feed wild boars. However, the interest of people of pursuing venison and changing taste is increasing. The method for domesticating wild boars is a task that people pay attention to. The method includes: choosing long and white sows or big and white sows, carrying out crossing breeding through crossing generation I, backcrossing generation II, horizontal crossing generation I, and side crossing generation II of the sows and wild boars, total 6 generations. 12-16 months are required for one generation; or choosing duroc sows, carrying out crossing breeding through crossing generation I, backcrossing generation I, horizontal crossing generation II within lines, and horizontal crossing generation III among lines of the duroc sows and wild boars, total 7 generations, 12-14 months are required for each generation. The crossing breeding and feeding method of wild boars is used for crossing breeding and feeding.

The invention provides a molecular marker, and particularly relates to cloning of a pig ECI1 gene segment and application as the molecular marker. A nucleotide sequence of the molecular marker is shown in SEQ ID NO.1; a C157-T157 base mutation is formed at the 157th bit, resulting in Bsm I-RFLP (restricted fragment length polymorphisms).

The present invention relates to a molecular marking method for pig litter size. The method comprises the steps of pig genome DNA extraction, exon 6 primer design of sex hormone binding globulin (SHBG) genes, amplification in vitro and genotype detection. The present invention first discovers pig SHBG genes, discovers the obvious relationship between the polymorphism of exon 6 and pig litter size, and develops a restriction fragment enzyme digestion polymorphism detection method for detecting exon 6 mutational sites. The method can be used for the auxiliary selection of litter size during pig breeding, can realize the early selection of parent pigs and even can accurately select parent pigs when the pigs are born. Generation interval is largely shortened, and selection progress is accelerated. The method has the advantages of simple operation, low condition requirements in polymerase chain reaction processes, short amplification fragment length (217bp), easy amplification, high amplification efficiency and accurate genotype judgement.

The invention discloses an in vitro embryo production method by lamb superovulation oocytes. The production method provided by the invention is accomplished by the following technical scheme: carrying out in vitro culture on oocytes acquired from lamb body in a maturation medium and maturing into ova, carrying out in vitro fertilization and culture so as to make the ova grow into embryos, and transplanting survived embryos into a surrogate ewe body for breeding. In comparison with the prior art, the method provided by the invention is utilized to adopt the oocytes acquired after the lamb superovulation treatment for the in vitro embryo production and has advantages of large quantity of the acquired oocytes, high production efficiency of embryo and short time for breeding lamb offspring. Therefore, the method provided by the invention has an edge in the application of large scale in vitro embryo production.

The invention discloses a molecular-assisted-selection cultivation method for a green-shin recessive-white-feather chicken line, and belongs to the technical field of molecular breeding. The molecular-assisted-selection cultivation method is adopted for authenticating the genotypes of dominant white feather sites of chickens, the genotypes of the dominant white feather sites can be rapidly determined, the green-shin recessive-white-feather chicken line with the genotypes being ii is produced, troubles of test cross breeding can be avoided, a generation interval is shortened, a breeding process is accelerated, and cultivation cost is reduced. The green-shin recessive-white-feather chicken line cultivated in the molecular-assisted-selection cultivation method has tender and delicious meat, is high in production performance, can be used as commercial chickens to be put into production directly, and can also be applied by being matched with green-shin jute-feather chickens.

The invention discloses a method for cultivating a dominant-green-shin white feather broiler strain based on molecular assistant selection and belongs to the technical field of molecular breeding. According to the method for cultivating the dominant-green-shin white feather broiler strain based on molecular assistant selection, the method of molecular assistant selection is adopted for appraisal of a genotype of a broiler dominant white feather locus, so that the genotype of the white feather locus is rapidly judged, the dominant-green-shin white feather broiler strain with the II genotype is produced, complexity of test cross selective breeding is avoided, a generation interval is shortened, the breeding progress is accelerated, and therefore the cultivation cost is reduced. Meat of the dominant-green-shin white feather broiler strain cultivated through the method is fine and tender, taste of the meat of the broiler strain is delicious, the production performance of the broiler strain is high, color feather residues do not exist on a carcass, and therefore the carcass is beautiful. In addition, the strain can serve as a male parent and be hybridized with a high-yield laying hen strain or a fast-growing large broiler strain in a matched mode so that high-yield or fast-growing large dominant-green-shin white feather broilers can be produced respectively.

The invention discloses a cultivating method of a laying hen with low egg yolk cholesterol content. The cultivating method of the laying hen with the low egg yolk cholesterol content comprises the following steps: 1, using deoxyribonucleic acid (DNA) sequence containing very low density lipoprotein receptor (VLDLR) gene of the laying hen as a template, designing a primer, and using polymerase chain reaction(PCR) to restriction fragment length polymorphism (RELP) method to conduct sample hen individual genotyping; 2, using a gene effect analysis model to determine select and remain genotype; 3, establishing a family of cock and hen individuals conforming to the select and remain genotype; 4, extracting DNA of offspring of hens of a select and remain genotype family, using the primer in the step 2 to conduct PCR-RFLP analysis, and selecting and remaining hens of the select and remain genotype in the step 2; 5, repeating the step 3 and the step 4 subculture and seed selection at least three generations, and obtaining laying hens with the low egg yolk cholesterol content. The cultivating method of the laying hen with the low egg yolk cholesterol content adopts a VLDLR gene molecular marker assisted select breeding method, egg yolk cholesterol contents of the cultivated laying hens can decrease more than 20 percentages compared with the original level.

The invention discloses a SNP loci combination for growth performance of meat sheep and an application thereof, and relates to the technical field of gene breeding. According to the invention, 2036 SNP loci are provided, and physical position information of the 2036 SNP loci is determined based on comparison of sheep v4.0 genome sequences. The SNP loci are SNP0001-SNP2036. Genetic evaluation is performed on sheep individuals based on the 2036 SNP loci, thereby achieving individual selection on growth traits difficult to measure in the early stage, shortening generation interval, accelerating breeding process and thus saving substantial breeding cost.

The invention discloses a screening method of chicken breeding materials, belonging to the field of agricultural livestock and poultry breeding. The screening method of chicken breeding materials comprises the following steps: 1) extracting genomic DNA; 2) performing microsatellite mark and PCR reaction; 3) performing mtDNA PCR reaction and sequencing; 4) performing statistical analysis; and 5) selecting breeding material. The invention adopts molecular biotechnology to analyze genetic diversity and genetic distance of breeding material, selects individuals with rich genetic diversity as breeding material, ensures rich genetic information, reduces blindness and risk of breeding, reduces breeding time, improves breeding efficiency and reduces breeding cost.

The invention relates to a method for breeding lean-type Chinese Huai pigs in a multi-gene pyramiding manner based on growth traits thereof. The method comprises the following operating steps: 1, carrying out DNA (deoxyribonucleic acid) extraction on the genome of the pig; 2, designing primers, more particularly, designing the PCR (polymerase chain reaction) amplification primers according to the gene sequences of insulin-like growth factor-I (IGF-I), pituitary specific transcription factor-I (PIT-I), liver X receptor alpha (LXR alpha) and melanocortin-4 receptor (MC4R); 3, carrying out the PCR; 4, carrying out restriction fragment length polymorphism (RFLP); and 5, carrying out the correlation analysis on the polymorphism and growth traits of genes. According to the analysis, the method can determine that the single growth rate of the pyramided gene with the gene type thereof being AADDGGFF is the highest one; the method can prevent the genes of other offsprings from being isolated after the selective reservation for breeding, thus achieving the optimal effect of the growth traits of the offsprings on the four gene types; and the method can stabilize the inheritance, particularly lead the genes of the growth traits to become homozygous within one generation, thus accelerating the cultivation of the lean-type Chinese Huai pigs.

The invention discloses a breeding method for a high-yield black-skin chicken strain and belongs to the technical field of molecular breeding. The genotype of a black-skin site of a chicken is authenticated with a molecular marker-assisted selection method, so that the genotype of the site can be quickly determined, and the shortcomings of complicated testcross, long generation interval and the like in conventional breeding are overcome. The method disclosed by the invention is used for producing the high-yield black-skin chicken strain of which the genotype is FmFm, so that the generation interval is shortened, the breeding progress is accelerated, and the breeding cost is reduced; furthermore, the egg laying performance of the strain is high, and the nutritional value of eggs is high; the black-skin characters of culled hens are uniform, and carcasses are more attractive. Furthermore, the strain is used as a male parent hybridized with high-laying-rate chickens, so that black-skin chickens with higher production performance can be produced.

An ultrasonic wave transceiving device is provided. The device includes a transmitter for transmitting at least one kind of first detection data ultrasonic signal and a plurality of kinds of second detection data ultrasonic signals, a receiver for receiving echo signals of the first and second detection data ultrasonic signals, and first and second information generators for generating first and second information based on the echo signal of the first and second detection data ultrasonic signals, respectively. The transmitter transmits the first and second detection data ultrasonic signals which transmission period of the first detection data ultrasonic signal intervenes between transmission periods of the second detection data ultrasonic signals in time axis. The second information generator generates the second information by using the echo signals of the second detection data ultrasonic signals.

The invention discloses a meat sheep reproductive performance SNP locus combination and an application thereof, and relates to the technical field of gene breeding. The locus combination provided by the invention comprises 2917 SNP loci, the physical position information of the SNP loci is determined based on sheep v4.0 genome sequence comparison, the SNP loci are SNP0001-SNP2917, and genetic evaluation is performed on sheep individuals based on the 2917 SNP locus combination. Individual selection can be performed on reproductive traits difficult to measure in the early stage, the generation interval is shortened, the breeding process is accelerated, and therefore, a large amount of breeding cost is saved.

The invention discloses a fast balance breeding method for native hen varieties, and belongs to the technical field of poultry breeding. According to the method, a hen strain with 50-percent high-egg-laying consanguinity is subjected to hybridizing matching with the hen strain with 75-percent high-egg-laying consanguinity to obtain a high-quality hen strain with 62.5-percent high-egg-laying consanguinity, the genetic negative correlation properties between the egg quality and the high-egg-laying properties can be well balanced through the high-egg-laying consanguinity contents of the matching strain, the egg laying properties are improved, meanwhile, the inherent egg quality of the native hens are maintained to the maximum degree, and the optimum high yield and high quality are realized. The progenitor male parent or female parent pure strains adopting high-yield hen variety matching combination by external consanguinity are imported, the genotype homogeneity can be favorably accelerated, the matching power of the matching strain is improved, meanwhile, a molecular marker auxiliary selection technology is adopted, the early stage selection is carried out, the generation interval is shortened, and the breeding process is accelerated.

The invention relates to a breeding method of a new Berkshire pig disease-resistant variety. The breeding method comprises the steps that firstly, weaned piglets of a Berkshire pig core group are subjected to Escherichia coli infection experiments by adopting a feed adding method, according to a phenotype identification result, a Berkshire pig Escherichia coli disease-resistant breeding base group is established; secondly, the base group is subjected to molecule breeding with general resistance to diseases by using a proprietary technology obtained in the early stage, meanwhile characters such as breeding performance, growing speed, lean meat percentage and carcass quality are detected and selected, and a first generation is built; thirdly, a group continuous progeny breeding and molecular marker assisted selection method is adopted, after four to five generations, an established breeding aim is achieved step by step, and a new variety core group is established. The new Berkshire pig disease-resistant variety has the advantages of resistance to weaned piglet diarrheal diseases, high stress resistance, high environmental adaptability and the like, and can further provide guidance for disease-resistant breeding and new variety selection and breeding of other pig varieties.

The invention discloses a quick balanced breeding method of chicken breeds with high quality, and belongs to the technical field of poultry breeding. The method comprises the steps of crossing meat chicken cultivated including 50% of blood relationship of local chicken and meat chicken including 75% of blood relationship of the local chicken in a supporting way to obtain a meat chicken breeding series including 62.5% of blood relationship of the local chicken. The external blood relationship content proportion of the local chicken and fast-growing chicken of the breeding series can well balance the negative correlation of inheritance between the meat quality and the growing speed, the inherit meat quality and flavor of the local chicken can be kept maximally when the growing speed can be improved, and the optimized high yield and high quality can be realized; furthermore, either progenitor male parent or female parent pure line of the combination of foreign varieties can be introduced by importing the foreign blood relationship, so as to reduce the determination on combining ability, and accelerate the homozygosis of genotype; in addition, an auxiliary selecting technology of a molecular marker is adopted to develop early selection, therefore the generation interval can be shortened, and the breeding progress can be accelerated.

The invention belongs to the technical field of animal molecular marker preparation and specifically relates to a molecular marker related to the character of milk yield of milk goat and an application thereof. The molecular marker is acquired in the manner of cloning a goat H-FABP gene; the sequence table of the nucleotide sequence is shown as SEQ ID NO.1; an A / C mutation is located at the No.124 locus basic group shown as the sequence table SEQ ID NO.1; Sma I-PFLP polymorphism is caused by the mutation. The invention also discloses a preparation method for the molecular marker and an application of a polymorphism detection method. A new molecular marker is supplied for the marker auxiliary breeding of the milk goat.

The invention provides an SNP molecular marker related with pig growth velocity and application thereof. According to the SNP molecular marker disclosed by the invention, a TRPC1 gene is utilized as acandidate gene which can affect the pig growth velocity; a sequencing technique is utilized to screen and identify SNP loci related with the pig growth velocity to obtain 2 SNP loci which can affectthe pig growth velocity and a gene type effect; the 2 SNP loci are respectively arranged (595bp)th and (539bp)th positions of a sequence shown in SEQ ID NO.1; allelotype of the 2 SNP loci is C and T;furthermore, alleles of C and C and T and T of the 2 SNP loci are completely linked on a genome; thus, any SNP locus can be applied to breeding pig breed growth rate characters; furthermore, the SNP loci cannot be limited by ages, genders and breeds of pigs, can be applied to early breeding of the pigs and can quicken a breeding process.

The invention belongs to the technical field of rex rabbit breeding, and discloses a four-stage rex rabbit breeding method. According to the growth and clothing hair development rules of rex rabbits,the four-stage breeding method for rex rabbits which are 8 weeks old, 13 weeks old, 23 weeks old and 6 / 7 months old is created. When the rex rabbits are 8 weeks old, first selection is carried out, and the reserved breeder quantity is at least 400% the bred core group quantity; when the rabbits are 13 weeks old, second selection is carried out, and the reserved breeder quantity is at least 300% the bred core group quantity; when the rex rabbits are 23 weeks old, third selection is carried out, and the reserved breeder quantity is at least 150% the bred core group quantity; when female rabbitsare 6 months old and male rabbits are 7 months old, fourth selection is carried out, rabbits which have a weight of at least 3500.00 g and at least four pairs of teats are reserved as breeders, and the reserved breeder quantity is at least 100% the bred core group quantity. In the method, staged selection (four times of selection in total) is adopted, more breeders are reserved for fine selection,the selection difference is increased, and the selection response is improved.

The invention discloses a breeding method of a laying hen with low egg yolk cholesterol content. The breeding method comprises the following steps of (1) taking a DNA (deoxyribonucleic acid) sequence including chicken HMGCR (Hydroxymethyl glutaric acyl coenzyme) gene as a template, designing a pair of primers, and carrying out sample chicken individual gene parting by a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method; (2) utilizing a genetic effect analysis model to determine selected and remained genetype; (3) building a family from individual bodies of cocks and hens according with the selected and remained genetype; (4) extracting the DNA in later generation of the selected and remained family hen, carrying out PCR-RFLP analysis on the pair of primers in step (1), selecting and remaining the hens with selected and remained genetype in step (2); and (5) repeating steps (3) and (4) to continuously breed for at least three generations, so as to obtain the laying hen with low egg yolk cholesterol content. The HMGCR gene molecular marker is adopted to assist and select the breeding method; the generation interval is shortened; the breeding accuracy is improved; the breeding flock feeding capacity is reduced; and the feeding cost is also reduced.

The invention provides a specific amplification primer of a sika deer microsatellite site M027 and an application of the specific amplification primer, relates to the specific amplification primer ofthe sika deer microsatellite site M027 and the application of the specific amplification primer, and aims to provide the specific amplification primer of the sika deer microsatellite site M027 and theapplication of the specific amplification primer. The nucleotide sequence of the primer of the sika deer microsatellite site M027 is as shown in SEQ.ID.NO:1 and SEQ.ID.NO:2. The sika deer microsatellite site M027 is applied to analysis of the growth traits of cornu cervi pantotrichum. The growth traits of the cornu cervi pantotrichum are selected according to genotypes, high-yield trait cornu cervi pantotrichum individuals and low-yield cornu cervi pantotrichum individuals can be screened, the accuracy is high, the operation is simple, the expense is low, the selection of the growth traits ofthe cornu cervi pantotrichum can be started at the early stage of life of sika deer, the generation interval is shortened, the selection intensity is improved, and the breeding process of the sika deer is accelerated. The specific amplification primer disclosed by the invention is suitable for the field of breeding of the sika deer.

The invention discloses a gene chip, a molecular probe combination and a kit for analyzing the characteristics of sheep wool and application, and relates to the technical field of biology. A 2849-SNP-site combination for analyzing the characteristics of the sheep wool and the molecular probe combination, the gene chip and the kit prepared on the basis of the site combination are provided, the characteristics difficult to measure in the early stage are detected from the gene level, the breeding process can be accelerated, the breeding cost can be saved, and the molecular probe combination, the gene chip and the kit can be used for screening and identifying sheep varieties, reconstructing sheep pedigree, protecting germplasm resources and improving the germplasm resources.

The invention belongs to the technical field of animal molecular marker preparation and specifically relates to a molecular marker related to the character of butter-fat content of milk goat and an application thereof. The molecular marker is acquired in the manner of cloning a goat SPARC gene; the sequence table of the nucleotide sequence is shown as SEQ ID NO.1; a G / A mutation is located at the No.142 locus basic group shown as the sequence table SEQ ID NO.1; Bgl II-RFLP polymorphism is caused by the mutation. The invention also discloses a preparation method for the molecular marker and an application of a polymorphism detection method. A new molecular marker is supplied for the marker auxiliary breeding of the milk goat.

The invention discloses a specific amplification primer for microsatellite locus M900 of sika deer and an application of the specific amplification primer. The nucleotide sequence of the specific amplification primer for microsatellite locus M009 is shown in SEQ. ID. NO: 1 and SEQ. ID. NO: 2. The microsatellite locus M009 for the sika deer is applied to the analysis of antler growth traits in thesika deer. The growth traits of the antler are selected according to the genotype, and individuals with high yield traits and low yield traits of the antler can be screened, high accuracy, simple operation and low cost are achieved, the growth traits of the antler can be selected early in the life of the sika deer, the generation interval is shortened, the selection intensity is improved, and thebreeding process of the sika deer is sped up; the specific amplification primer for the microsatellite locus M900 of the sika deer and the application of the specific amplification primer are applicable to the field of breeding of the sika deer.

The invention discloses a molecular marker of fat deposition and meat quality traits of a lean meat type Dingyuan pig strain. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:1, a398th base in the above sequence is a base mutation site, and the base mutation site is G or C. The invention discloses a primer pair for detecting the above molecular marker. The invention disclosesa molecular marking method for the fat deposition and meat quality traits of the lean meat type Dingyuan pig strain. The method comprises the following steps: extracting genomic DNA containing the above molecular marker in pigs; performing a polymerase chain reaction by using the above primer pair, the genomic DNA and a PCR reaction buffer solution to obtain an in-vitro amplification product; performing an enzyme digestion reaction on the in-vitro amplification product by using a restriction enzyme to obtain an enzyme-digested product; and performing polymorphism detection on the enzyme-digested product to obtain genotypes of each individual. The invention discloses an application of the above molecular marker and / or the above primer pair in the breeding of live pigs of the lean meat typeDingyuan pig strain.

The invention discloses a molecular marker method of a beef cattle fat color trait. The method comprises steps as follows: DNA extraction of bovine genome, design of a third exon primer of a BCO2 (beta-carotene oxygenase 2) gene, in-vitro amplification and genotype detection. The method can be used for assistant selection of the fat color trait in cattle breeding and can realize early selection of breeder cattle; with the adoption of the gene pyramiding method, the good genotype of the BCO2 gene can be stabilized after one generation, that is, homozygosis of a good gene of the fat color trait of a beef cattle strain is realized through selection of one generation, the generation interval is greatly shortened, and the selection process is accelerated. The molecular marker method is simple and convenient to operate, condition requirements in the polymerase chain reaction process are low, the length of amplified fragments is short (525bp), amplification is easier, and the amplification efficiency and the judgement accuracy of the genotype are improved.

The invention relates to the technical field of corn breeding, in particular to a method for breeding drought-enduring male parent germplasm based on a corn PB group. The method comprises the following steps: mixing PB group drought-resistant corn germplasm with a male parent material to construct a germplasm improvement basic group, and selfing to generate an S1 generation; performing inter-ear-row drought resistance screening on the S1 generation in the seedling stage, dividing the screened drought-resistant ear rows into two parts, respectively transplanting the two parts, selecting ear rows correspondingly planted in a normal irrigation area according to the identification result of the drought resistance of the ear rows after planting in an arid area, and performing selfing to obtain an S2 generation and form a DH1 line; taking a small amount of DH1 lines to form a test mating group TC1, performing multi-environment drought resistance phenotype identification on the test mating group TC1 and parent DH1 lines thereof, and respectively establishing whole genome selection models; a whole genome selection model is utilized to predict phenotypes of a large number of DH1 lines, and a screened excellent inbred line is a drought-resistant corn male parent germplasm. The invention aims to solve the problems of long breeding period, low selection efficiency and difficulty in balance of yield and drought tolerance of the existing corn male parent germplasm.

The invention discloses a breeding method capable of increasing chicken specialized dam line reproductive performance and belongs to chicken breeding methods. The method includes: building a F1 generation colony, building a breeding colony, screening the breeding colony, building a F2 generation colony, continuously breeding to the F(N) generation colony and the like. The method has the advantagesthat systematic breeding is performed by using small-scale colony feeding and natural mating, generation interval is lowered effectively, and the method is convenient and practicable in operation, fast in genetic progress and capable of guaranteeing animal welfare and effectively increasing productivity; the chicken specialized dam line bred by the method is high in reproductive performance, lowin pecking addiction rate and high in survival rate, the colony does not like fight and is quite and high in productivity, and the method has significant scientific and economic values in the increasing of the chicken breeding technology of China and the lowering of chicken breeding cost.

The invention relates to a molecular marker method for growth traits of pigs. The operation steps are as follows: extract porcine genomic DNA from porcine ear margin tissue; design a pair of primers between -1201bp and -654bp of the initiation transcription site of porcine calcium-binding protein 2 (CALB2) gene, that is, upstream primers and downstream primers Obtain the 548bp long in vitro amplification product between the upstream -1201bp and -654bp of the pig CALB2 gene transcription start site by polymerase chain reaction; Carry out the restriction endonuclease HhaI enzyme digestion reaction to the in vitro amplification product; The cut product was tested for polymorphism (RLFP), and the AA type was obtained as the genotype with excellent growth speed, that is, the mutant type. Therefore, the piglets with the AA type were selected for breeding, and the growth and fattening cycle of the AA type pigs was shorter than that of the GG type. Pigs shortened by 3.6 to 4.0 days.