77results about How to "Shorten the generation interval" patented technology

The invention provides a molecular marker, and particularly relates to cloning of a pig ECI1 gene segment and application as the molecular marker. A nucleotide sequence of the molecular marker is shown in SEQ ID NO.1; a C157-T157 base mutation is formed at the 157th bit, resulting in Bsm I-RFLP (restricted fragment length polymorphisms).

The invention discloses an in vitro embryo production method by lamb superovulation oocytes. The production method provided by the invention is accomplished by the following technical scheme: carrying out in vitro culture on oocytes acquired from lamb body in a maturation medium and maturing into ova, carrying out in vitro fertilization and culture so as to make the ova grow into embryos, and transplanting survived embryos into a surrogate ewe body for breeding. In comparison with the prior art, the method provided by the invention is utilized to adopt the oocytes acquired after the lamb superovulation treatment for the in vitro embryo production and has advantages of large quantity of the acquired oocytes, high production efficiency of embryo and short time for breeding lamb offspring. Therefore, the method provided by the invention has an edge in the application of large scale in vitro embryo production.

The invention discloses a molecular-assisted-selection cultivation method for a green-shin recessive-white-feather chicken line, and belongs to the technical field of molecular breeding. The molecular-assisted-selection cultivation method is adopted for authenticating the genotypes of dominant white feather sites of chickens, the genotypes of the dominant white feather sites can be rapidly determined, the green-shin recessive-white-feather chicken line with the genotypes being ii is produced, troubles of test cross breeding can be avoided, a generation interval is shortened, a breeding process is accelerated, and cultivation cost is reduced. The green-shin recessive-white-feather chicken line cultivated in the molecular-assisted-selection cultivation method has tender and delicious meat, is high in production performance, can be used as commercial chickens to be put into production directly, and can also be applied by being matched with green-shin jute-feather chickens.

The invention discloses a SNP loci combination for growth performance of meat sheep and an application thereof, and relates to the technical field of gene breeding. According to the invention, 2036 SNP loci are provided, and physical position information of the 2036 SNP loci is determined based on comparison of sheep v4.0 genome sequences. The SNP loci are SNP0001-SNP2036. Genetic evaluation is performed on sheep individuals based on the 2036 SNP loci, thereby achieving individual selection on growth traits difficult to measure in the early stage, shortening generation interval, accelerating breeding process and thus saving substantial breeding cost.

The invention discloses a breeding method for a complete set line of red feather pink shell laying hens and provides a primer pair for identifying a red feather-induced mutant genotype of white leghorns. The primer pair consists of a single-stranded DNA molecule shown as a sequence 2 in a sequence table and a single-stranded DNA molecule shown as a sequence 3 in the sequence table. The primer pairis characterized in that primers are designed according to nucleotide sequences on the upstream and downstream of 18,288,303th deoxynucleotide on a positive-sense strand of an eleventh chromosome ina chicken reference genome Gallus_gallus-4.0 version sequence information disclosed by NCBI; a locus genotype is detected by restriction fragment length polymorphism, the white leghorns with homozygous red feather-induced mutant genotype are propagated, and pure line female parents are bred; hybrid hens obtained by female parents and Rhode Island Red male parents are completely in a red feather phenotype; the breeding method can meet market demand and has a broad popularization prospect.

The invention belongs to the technical field of animal molecular marker preparation and specifically relates to a molecular marker related to the character of butter-fat content of milk goat and an application thereof. The molecular marker is acquired in the manner of cloning a goat SPARC gene; the sequence table of the nucleotide sequence is shown as SEQ ID NO.1; a G / A mutation is located at the No.142 locus basic group shown as the sequence table SEQ ID NO.1; Bgl II-RFLP polymorphism is caused by the mutation. The invention also discloses a preparation method for the molecular marker and an application of a polymorphism detection method. A new molecular marker is supplied for the marker auxiliary breeding of the milk goat.

The invention discloses a specific amplification primer for microsatellite locus M900 of sika deer and an application of the specific amplification primer. The nucleotide sequence of the specific amplification primer for microsatellite locus M009 is shown in SEQ. ID. NO: 1 and SEQ. ID. NO: 2. The microsatellite locus M009 for the sika deer is applied to the analysis of antler growth traits in thesika deer. The growth traits of the antler are selected according to the genotype, and individuals with high yield traits and low yield traits of the antler can be screened, high accuracy, simple operation and low cost are achieved, the growth traits of the antler can be selected early in the life of the sika deer, the generation interval is shortened, the selection intensity is improved, and thebreeding process of the sika deer is sped up; the specific amplification primer for the microsatellite locus M900 of the sika deer and the application of the specific amplification primer are applicable to the field of breeding of the sika deer.

The invention discloses a molecular marker of fat deposition and meat quality traits of a lean meat type Dingyuan pig strain. The nucleotide sequence of the molecular marker is shown in SEQ ID NO:1, a398th base in the above sequence is a base mutation site, and the base mutation site is G or C. The invention discloses a primer pair for detecting the above molecular marker. The invention disclosesa molecular marking method for the fat deposition and meat quality traits of the lean meat type Dingyuan pig strain. The method comprises the following steps: extracting genomic DNA containing the above molecular marker in pigs; performing a polymerase chain reaction by using the above primer pair, the genomic DNA and a PCR reaction buffer solution to obtain an in-vitro amplification product; performing an enzyme digestion reaction on the in-vitro amplification product by using a restriction enzyme to obtain an enzyme-digested product; and performing polymorphism detection on the enzyme-digested product to obtain genotypes of each individual. The invention discloses an application of the above molecular marker and / or the above primer pair in the breeding of live pigs of the lean meat typeDingyuan pig strain.

The invention relates to the technical field of corn breeding, in particular to a method for breeding drought-enduring male parent germplasm based on a corn PB group. The method comprises the following steps: mixing PB group drought-resistant corn germplasm with a male parent material to construct a germplasm improvement basic group, and selfing to generate an S1 generation; performing inter-ear-row drought resistance screening on the S1 generation in the seedling stage, dividing the screened drought-resistant ear rows into two parts, respectively transplanting the two parts, selecting ear rows correspondingly planted in a normal irrigation area according to the identification result of the drought resistance of the ear rows after planting in an arid area, and performing selfing to obtain an S2 generation and form a DH1 line; taking a small amount of DH1 lines to form a test mating group TC1, performing multi-environment drought resistance phenotype identification on the test mating group TC1 and parent DH1 lines thereof, and respectively establishing whole genome selection models; a whole genome selection model is utilized to predict phenotypes of a large number of DH1 lines, and a screened excellent inbred line is a drought-resistant corn male parent germplasm. The invention aims to solve the problems of long breeding period, low selection efficiency and difficulty in balance of yield and drought tolerance of the existing corn male parent germplasm.

The invention relates to a molecular marker method for growth traits of pigs. The operation steps are as follows: extract porcine genomic DNA from porcine ear margin tissue; design a pair of primers between -1201bp and -654bp of the initiation transcription site of porcine calcium-binding protein 2 (CALB2) gene, that is, upstream primers and downstream primers Obtain the 548bp long in vitro amplification product between the upstream -1201bp and -654bp of the pig CALB2 gene transcription start site by polymerase chain reaction; Carry out the restriction endonuclease HhaI enzyme digestion reaction to the in vitro amplification product; The cut product was tested for polymorphism (RLFP), and the AA type was obtained as the genotype with excellent growth speed, that is, the mutant type. Therefore, the piglets with the AA type were selected for breeding, and the growth and fattening cycle of the AA type pigs was shorter than that of the GG type. Pigs shortened by 3.6 to 4.0 days.