62results about How to "Speed ​​up breeding progress" patented technology

The invention discloses a molecular marking method selecting goat growth traits using nucleophosmin gene. According to the method, a goat gene group DNA sequence is used as a template, under the condition of existence of Taq DNA polymerase, buffer environment, Mg <2+>, and dNTPs, four pairs of primer are used for amplifying a Nucleophosmin (NPM) gene 5'UTR region and exon 1 under the condition of PCR condition, and the size of a target segment of an amplified product is determined according to agarose gel electrophoresis; an amplified site of primer P3 is found lacking of 18bp basic group through detection of PCR amplification products of primers P1-P4 by use of polyacrylamide gel electrophoresis, then genotyping and gene frequency analysis are performed on the amplification product of the primer P3, and associated analysis on the amplification production of the primer P3 and growth traits of a milk goat and a Boer goat in the central Shaanxi plain is performed. Results indicate that the SNPs site of the Nucleophosmin gene exon 1 detected by the primer P3 can be used as a molecular mark for goat growth trait selection.

The invention discloses a method for selecting milk production characters to breed a milk goat by utilizing the double gene polymerization effect. Milk goat genome DNA serves as a template, two pairs of primers are used for amplifying the exon 9 of a gene of a PRLR and the intron 1 of a gene of an ELF5 respectively, agarose gel electrophoresis with the concentration of 1.5% is used for judging the sizes of the amplified products, the locus mutations of the amplified products of the two pairs of primers are screened based on the DNA sequencing technique, then, the agarose gel electrophoresis with the concentration of 3.5% is used for carrying out genetic typing and gene frequency analyzing on SNPs of the two locuses of the gene of the PRLR and the gene of the ELF5, the relation between different gene type combinations and the milk production characters is analyzed, and the optimal gene combination is screened out.

The invention discloses a method for evaluating the quality of eggshell colloidal protection membranes with different colors. The method comprises: measuring the change of an XYZ color space by usinga spectrophotometer before and after an eggshell colloidal protection membrane is stained with a MST colloidal protection membrane blue, converting the XYZ color space into a RGB color space through aformula, finally converting into opacity, and evaluating the quality of the eggshell colloidal protection membrane by calculating the change of the opacity before and after the staining of the eggshell colloidal protection membrane, wherein the higher the change of the opacity before and after the staining of the eggshell colloidal protection membrane, the better the quality of the eggshell colloidal protection membrane, and the smaller the change of the opacity before and after the staining of the eggshell colloidal protection membrane, the worse the quality of the eggshell colloidal protection membrane. According to the present invention, the method has characteristics of simple operation, low cost and short detection time, and can be generalized to various poultry eggs with colloidal protection membranes, such that the variety with the excellent colloidal protection membrane and the variety with the poor colloidal protection membrane quality can be hybridized so as to select the variety with the excellent colloidal protection membrane quality, reduce the bacterial invasion chance on poultry eggs, and prolong the storage and fresh-keeping time of poultry eggs.

The invention discloses a method for detecting insertion-deletions polymorphism of sheep FTH-1 (ferritin heavy polypeptide1) genes by utilizing PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) and application thereof. The method comprises the following steps: taking to-be-detected sheep whole genome DNA containing FTH-1 genes as a template, performing PCR amplification on the sheep FTH-1 gene segments, and performing detection and genotyping on the PCR product by utilizing the SSCP technology; and identifying the 639th locus insertion-deletions polymorphism of the sheep FTH-1 genes according to electrophoretic results. The method is a method for screening and detecting a molecular genetic marker which is closely related to reproductive performances of sheep on the DNA level so as to be used for assistant selection and molecular breeding of the sheep and acceleration of sheep stock breeding.