56 results about "Kit gene" patented technology

The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and / or pseudogenes.

Gene chip for inspecting HIV to reverse transcriptase inhibitor resistance and reagent kit are disclosed. The gene chip consists of solid-phase carrier and oligonucleotide probe fixed on the solid-phase carrier, which comprises mutation site DNA fragment selected in HIV reversed transcriptive enzyme gene area, PCR amplifying for sample cDNA to be inspected by primer, purification marking for amplified product and hybridizing with the gene chip, acquiring hybridized signal by gene chip scanner, analyzing and appraising to determine medicine-tolerance site mutation. It's simple, accurate and repeated.

The invention relates to specific polymerase chain reaction (PCR) primers for detecting various serotype pathogenic bacteria of legionella pneumophilia, a method for quickly detecting by adopting a multi-PCR kit, and application. The kit comprises 10*PCR reaction liquid, MgCl2, dNTP, primers, and DNA polymerase; wherein the sequences of the primers is one or both of (1) and (2), wherein (1) wzt or wzm specific nucleotide sequences of legionella pneumophila serogroups 1, 4, 6, 10 and 13; and (2) complementary DNA sequences of DNA sequences selected from (1). The primers have high practicability for wzt specific nucleotide of common legionella pneumophila serogroups, and a PCR kit, a gene chip or a microarray comprising the nucleotide; and the PCR kit is easy and convenient to prepare, short in detection period, high in speed and accuracy, and strong in operability, detection cost is reduced, and the PCR kit is suitable for industrial production.

The present invention provides probes (P1) to (P4) which can detect different polymorphisms of c-kit gene in a simple and highly reliable manner: (P1) an oligonucleotide which comprises a nucleotide sequence complementary to a nucleotide sequence having a 4- to 50-nucleotide length and containing nucleotide-105 to nucleotide-108 in SEQ ID NO:1 and which has a nucleotide complementary to nucleotide-105 at the 3'-terminal region; (P2) an oligonucleotide which comprises a nucleotide sequence having a 7- to 50-nucleotide length and containing nucleotide-102 to nucleotide-108 in SEQ ID NO:1 and which has a nucleotide corresponding to nucleotide-102 at the 5'-terminal region; (P3) an oligonucleotide which comprises a nucleotide sequence complementary to a nucleotide sequence having a 8- to 50-nucleotide length and containing a nucleotide-127 to nucleotide-134 in SEQ ID NO:1 and which has a nucleotide complementary to nucleotide-134 at the 5'-terminal region; and (P4) an oligonucleotide which comprises a nucleotide sequence having a 5- to 50-nucleotide length and containing nucleotide-123 to nucleotide-127 in SEQ ID NO:1 and which has a nucleotide corresponding to nucleotide-123 at the 5'-terminal region.