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56 results about "Kit gene" patented technology

The KIT gene encodes a tyrosine kinase receptor and is part of signaling pathways that control multiple cellular processes, including cell proliferation, survival, and migration. The KIT gene is also important for the development of several cell types, such as blood cells, mast cells, germ cells, interstitial cells of Cajal, and melanocytes.

Universal RT-coupled PCR method for the specific amplification of mRNA

The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and / or pseudogenes.
Owner:HEALTH RES INC

Universal RT-coupled PCR method for the specific amplification of mRNA

InactiveUS20030186288A1Genomic DNA amplification is avoidedSugar derivativesMicrobiological testing/measurementForward primerSpecific detection
The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and / or pseudogenes.
Owner:HEALTH RES INC

Primer, probe, locked nucleic acid probe, kit and detection method for detecting C-kit gene mutation

The invention discloses a primer, a probe, a locked nucleic acid probe, a kit and a detection method for detecting C-kit gene mutation, belonging to the technical field of biology. The primer and probe for detecting C-kit gene mutation comprise at least one of a primer and probe of a C-kit gene No.9 exon for detecting a No.9 exon of a C-kit gene, a primer and probe of a C-kit gene No.11 exon for detecting a No.11 exon of the C-kit gene, a primer and probe of a C-kit gene No.13 exon for detecting a No.13 exon of the C-kit gene, and a primer and probe of a C-kit gene No.17 exon for detecting a No.17 exon of the C-kit gene. The kit comprises the primer and the probe. The primer and probe can be used for highly-sensitively detecting whether the C-kit gene has mutation, meanwhile, the sensitivity of detecting the C-kit gene mutation can be greatly improved by adopting the locked nucleic acid probe, and whether the C-kit gene is mutated can be accurately detected by adopting the kit.
Owner:WUHAN BIOTECH GENE ENG

Nucleotide specific to Vibrio fluvialis O2, O4, O13, O15 and O18 and application thereof

The invention relates to a nucleotide specific to Vibrio fluvialis O2, O4, O13, O15 and O18 serotypes and an application thereof. The nucleotide comprises 1) at least one of nucleotides shown in SEQIDNO: 1-14; and 2) at least one of nucleotides shown in SEQIDNO: 1-14. The nucleotides can be used for preparing PCR (polymerase chain reaction) kits and gene chips for detecting Vibrio fluvialis. The nucleotide specific to Vibrio fluvialis O2, O4, O13, O15 and O18 serotypes disclosed by the invention, as well as a PCR kit and a gene chip which contain the nucleotide, are strong in practicability, and the PCR kit is simple in preparation method, short in detection cycle, rapid in speed, strong in maneuverability, convenient for industrialized production, and low in detection cost; the accuracy is high; and the sensitivity is high.
Owner:NANKAI UNIV

Method for breeding kidding traits by utilizing 3-gene pyramiding effect

The invention discloses a method for breeding kidding traits by utilizing 3-gene pyramiding effect. The method comprises the following steps: amplifying a 3' untranslated region of a stem cell factor (SCF) gene, an exon 7 of a III type transmembrane tyrosine kinase receptor (KIT) gene and an intron 1 of a kiss (KISS1) gene respectively by using three pairs of primers and by taking goat genome DNA as a template; judging the size of each amplification product by using agarose gel electrophoresis at the concentration of 1.5%; screening the site mutation of the amplification products of the three pairs of primers by a DNA sequencing technology; performing genetic typing and gene frequency analysis on the single nucleotide polymorphisms (SNPs) of three sites of the SCF gene, the KIT gene and the KISS1 gene by using polyacrylamide gel electrophoresis at the concentration of 10% and agarose gel electrophoresis at the concentration of 3.5%; analyzing the relationship between different genotype combinations and kidding quantity; and screening out the optimal genotype combination.
Owner:NORTHWEST A & F UNIV

PML-RARa gene fluorescence quantitative RT-PCR primer and probe and reagent kit

InactiveCN1995385AImprove induction rateReduce early deathMicrobiological testing/measurementReference genesFluorescence
The invention discloses a quantitative RT-PCR primer and probe and agent box of PML-RARa fusing gene mRNA fluorescence with BCR-ABL fusing gene primer and probe sequence as SEQ ID NO1-4 and internal reference gene primer and probe sequence as SEQ ID NO5-7, wherein the agent box contains cell cracking liquid, water, RT-PCR reacting liquid, internal reference G6PDHRT-PCR reacting liquid, BCR-ABL fusing gene detecting probe, G6PDH internal reference gene testing probe, composite enzyme, standard material and comparing material; the invention measures the expressive level of L-pattern, S-pattern and V-pattern mRNA simultaneously, rapidly and precisely, which improves inducing buffer rate and lessens early bleeding death.
Owner:SHANGHAI FOSUN PHARMA (GROUP) CO LTD +1

C-KIT gene multipoint mutation single tube fast detection method and kit

The invention discloses a C-KIT gene multipoint mutation single tube fast detection method and kit. The C-KIT gene multipoint mutation single tube fast detection method comprises 1, designing ARMS primers and a corresponding downstream primer, intermediary connection primer, general fluorescent probe and quenching probe, 2, mixing the ARMS primers, the corresponding downstream primer, intermediary connection primer, general fluorescent probe and quenching probe and a hot-start fast Taq enzyme system and carrying out amplification, and 3, detecting a fluorescence change of the reaction system to determine if point mutation exists. The detection method can be operated simply and can realize qualitative detection of multipoint mutation existence. The detection method utilizes the primers with low design difficulty, greatly reduces a primer synthesis cost, solves limitation of the existing ARMS technology, greatly improves detection throughput, realizes single tube reaction replacing the existing multi-tube detection and saves manpower and material resources.
Owner:GUANGZHOU HEAS BIOTECH CO LTD

Gene chip for inspecting IIIV P-reverse transcriptase inhibiting resistance and its reagent kit

Gene chip for inspecting HIV to reverse transcriptase inhibitor resistance and reagent kit are disclosed. The gene chip consists of solid-phase carrier and oligonucleotide probe fixed on the solid-phase carrier, which comprises mutation site DNA fragment selected in HIV reversed transcriptive enzyme gene area, PCR amplifying for sample cDNA to be inspected by primer, purification marking for amplified product and hybridizing with the gene chip, acquiring hybridized signal by gene chip scanner, analyzing and appraising to determine medicine-tolerance site mutation. It's simple, accurate and repeated.
Owner:TIANJIN BIOCHIP TECH CO LTD

Specific primer and kit for detecting common food allergens

The invention relates to a specific primer and a kit for detecting common food allergens, and particularly relates to a specific PCR (Polymerase Chain Reaction) primer for detecting food allergens and a method and application thereof for rapidly detecting the common food allergens with a multiplex-PCR kit. The kit comprises a 10*PCR reaction solution, MgC12, dNTP (Diethyl-Nitrophenyl Thiophosphate), a primer and DNA (Deoxyribonucleic Acid) polymerase, wherein the primer comprises the following specific nucleotide sequences: (1) MTD (Maximum Tolerated Dose) gene of celery, (2) mitochondrial 16S of fish, (3) Lectin gene of soybean and (4) Prudul gene of almond. The specific nucleotide, the PCR kit with the nucleotide, and the gene chip or a micro-array which are used for detecting the common food allergens are high in practicability; and the PCR kit is simple and convenient to prepare, needs a short detection period, has a high speed and high accuracy, is high in operability, can reduce detection costs and is prone to industrial production.
Owner:TIANJIN BIOCHIP TECH CO LTD

Primer group, kit and method for detecting AML related gene mutation

The invention discloses a primer group, a kit and a method for detecting AML related gene mutation, and belongs to the technical field of gene detection. The primer group provided by the invention comprises one or more of an FLT3 gene primer pair, a DNMT3A gene primer pair, an IDH1 gene primer pair, an IDH2 gene first primer pair, an IDH2 gene second primer pair, a KIT gene primer pair and an NPM1gene primer pair; the kit provided by the invention comprises the primer group and one or more of an FLT3 gene probe, a DNMT3A gene probe, an IDH1 gene probe, an IDH2 gene first probe, an IDH2 gene second probe, a KIT gene probe and an NPM1 gene probe. The detection method for detecting AML related gene mutation provided by the embodiment of the invention has the characteristics of high efficiency, simplicity, convenience, intuition and high sensitivity, and can be used for quantitatively analyzing the AML related gene mutation frequency.
Owner:CARRIER GENE TECH SUZHOU CO LTD

Zebrafish model for assessing gastrointestinal motility

The present invention has demonstrated the presence of the zebrafish KIT protein resulting from the expression of the kit gene in the zebrafish GI tract. As expression of the KIT protein is correlated with ICC in other species, the expression of the KIT protein in zebrafish indicates the presence of ICC in the zebrafish GI tract. ICC are required for spontaneous, coordinated contractions of GI smooth muscle. The present invention provides a zebrafish-based model system useful for elucidating the cellular and molecular mechanisms of gastrointestinal (GI) function and for identifying molecular targets for treating GI motility disorders in human.
Owner:NEW YORK THE RES FOUND OF STATE UNIV OF

Primer, kit as well as PCR method for detecting of D816V mutation site of C-KIT gene

The invention discloses a primer, a kit as well as a PCR method for detecting a D816V mutation site of a C-KIT gene. The primer comprises a wild-type specific forward primer, a mutant-type specific forward primer and a reverse primer which are shared by the wild-type specific forward primer and the mutant-type specific forward primer, wherein the wild-type specific forward primer has a sequence shown in SEQ No.17, the-mutant type specific forward primer has a sequence shown in SEQ No.14, and the shared reverse primer has a sequence shown in SEQ No.16. The kit has the advantages of being simple, rapid, accurate and cheap in detection; and a powerful instrument is provided for scientific research and clinical D816V site typing and gene mutation analysis of the C-KIT gene.
Owner:沈阳优吉诺生物科技有限公司

Nucleotide specific to wzt of Legionella pneumophila serogroup 1 and application thereof

The invention relates to a nucleotide specific to wzt of Legionella pneumophila serogroup 1 and application thereof. The nucleotide is a nucleotide shown as SEQ ID NO: 1 and / or a nucleotide shown as SEQ ID NO: 2 and is complementary with the nucleotides. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Legionella pneumophila serogroup 1, a gene chip or a micro-array. The nucleotide specific to the wzt of the Legionella pneumophila serogroup 1 provided by the invention as well as the PCR kit, the gene chip or the micro-array including the nucleotide have the advantages of strong practical applicability, simple preparation method of the PCR kit, short detection period, high speed, strong maneuverability, easiness for industrial production, low detection cost, high accuracy and high sensitivity.
Owner:NANKAI UNIV

A method for detecting c-kit gene mutation

The invention discloses a c. A method for detecting mutation of kit gene comprising the follow steps: S1, according to 9, 11, 13 and 17 c-Kit Exon DNA Sequences, designing Specific Amplification Primers; S2, PCR amplification of No. 9, 11, 13 and 17 c by using the specific amplification primers designed in step S1; Kit exon, and the PCR amplified products being digested by enzymes; S3, carrying out cyclic amplification on that enzymatic hydrolysate according to the sequence primer to obtain a Sanger fragment; S4, using the Sanger fragment obtained in the step S3, analyzing No.9, 11, 13 and 17c-Kit exon DNA sequence information through an automatic gene instrument, comparing with wild genotype to find out the mutation site. The invention designs the specific amplification primer, shortensthe length of the primer, improves the specific amplification sensitivity, and intuitively sees whether the target gene amplified by PCR is qualified through the specific amplification primer, therebydeciding whether to carry out the next step and avoiding wasting the reagent.
Owner:郑州海普医学检验所

Specific nucleotide for aeromonas hydrophila O44, O24, O25 and O28 and application thereof

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.
Owner:NANKAI UNIV

Gene chip for inspecting and grouping SARS coronavirus, its method and reagent kit

Gene chip for inspecting and parting SARS coronavirus, its method and reagent kit are disclosed. The gene chip consists of solid-phase carrier and oligonucleotide probe fixed on the solid-phase carrier, which comprises DNA fragment containing S gene mutation site selected from S gene sequence with one or multiple SARS coronavirus and DNA fragment selected from S gene conservative sequence of SARS coronavirus. The process is carried out by PCR amplifying for sample cDNA to be inspected, hybridizing with the gene chip, determining nucleotide kind by different fluorescent value and parting for SARS sample to be inspected. It's simple, accurate and repeated.
Owner:TIANJIN BIOCHIP TECH CO LTD

Nucleotide specific to hafinia alvei G5907, G5908, G5913 and G5916, and application of nucleotide

The invention relates to a nucleotide specific to hafinia alvei G5907, G5908, G5913 and G5916, and an application of the nucleotide. The nucleotide comprises one of nucleotides shown in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR kit and a gene chip for detecting hafinia alvei. The invention provides the nucleotide specific to hafinia alvei G5907, G5908, G5913 and G5916, as well as the PCR kit and the gene chip comprising the nucleotide. The technology has the advantages of simple and convenient operation method, short detection cycle, low detection cost, high accuracy and high sensitivity, and is suitable for industrial production.
Owner:NANKAI UNIV

Primers and method for detecting c-kit gene mutation

The invention provides primers for detecting c-kit gene mutation, and the primers include a forward primer and a backward primer for exon 8 and a forward primer and a backward primer for exon 17. The invention belongs to the technical field of biological detection, and the primers provided by the invention can specifically detect c-kit gene exons 8 and 17 mutations, and the specificity of the primers is good, and the accuracy is high, and the efficiency of the detection is increased.
Owner:GUANGZHOU KINGMED DIAGNOSTICS CENT

SgRNA for specific recognition of pig KIT gene, coding DNA thereof and kit, and application thereof

PendingCN110964725AChange coat colorChange-characterStable introduction of DNANucleic acid vectorKit geneGenetic engineering
The invention provides sgRNA for specific recognition of a pig KIT gene, a coding DNA thereof and a kit, and application thereof, belonging to the technical field of gene engineering. In the sgRNA, anucleotide sequence responsible for identifying a target fragment region is a sequence as shown in SEQ ID NO.1, a sequence as shown in SEQ ID NO.2 or a sequence as shown in SEQ ID NO.3; and the sgRNAenables a CRISPR / Cas9 gene editing system to delete redundant mutation copies of the KIT gene on a pig genome, so the KIT gene can normally express KIT protein with biological activity, and the hair color of a pig is changed.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI

Deafness-causing porcine KIT mutant gene and application thereof

The invention relates to the technical field of biology, particularly a deafness-causing porcine KIT mutant gene and application thereof. The invention is characterized in that the porcine KIT mutant gene has a c.2418T>A mutation as compared with the wild type KIT gene. The porcine KIT mutant gene can cause porcine deafness and appears dominant inheritance. When being used for preparing a deafness animal model and researching the deafness pathogenesis, the mutation has significant clinical instruction meanings for prevention, diagnosis and treatment of deafness.
Owner:ARMY MEDICAL UNIV

Nucleotides specific to Hafnia alveibifermentans G58097, G5898 and G5900 and their application

The invention relates to nucleotides specific to Hafnia alveibifermentans G58097, G5898 and G5900 and their application. The nucleotides include one type of nucleotides shown in SEQ ID NO: 1 to 6. These nucleotides are applicable to the preparation of PCR kits and gene chips used for detecting Hafnia alveibifermentans. The invention relates to the nucleotides specific to Hafnia alveibifermentans G58097, G5898 and G5900, and a PCR kit and a gene chip both including the nucleotides; an operating method is simple, a detection period is short, detection cost is lower, accuracy is high, sensitivity is high, and the nucleotides, the PCR kit and the gene chip are easy to industrially produce.
Owner:NANKAI UNIV

Molecular marker for diagnosis and prognosis judgment of canine breast tumor

The invention discloses a molecular marker for the diagnosis and prognosis judgment of a canine breast tumor. The molecular marker is an ICAM1 gene with a nucleotide sequence shown in the formula of SEQ ID NO: 1. The invention also discloses RT-qPCR detection primers for detecting ICAM1 gene expression. The primers have nucleotide sequences shown in the formulas of SEQ ID NO: 2-3. The invention also discloses a kit for diagnosis and prognosis determination of the canine breast tumor. The ICAM1 gene only is significantly low expressed in the malignant tumor group. The expression level of the ICAM1 gene in canine mammary gland tumor cells isolated from metastatic lesions is significantly different from that of primary tumor cells so that it is proved that the ICAM1 can identify benign and malignant canine breast tumors and is participated in the infiltration and metastasis of canine mammary tumors. Therefore, ICAM1 can be used as a molecular marker for the diagnosis and prognosis of canine breast tumors. The molecular marker related to canine mammary gland tumor metastasis has high sensitivity and specificity, can identify the nature of the canine breast tumor, can evaluate the prognosis, can determine the treatment plan in time, and can improve the survival rate of the diseased dog.
Owner:SOUTH CHINA AGRI UNIV

Method for identifying spotted tumor cattle variety by using KIT gene copy number variation genetic marker

The invention discloses a method for identifying a spotted tumor cattle variety by using a KIT gene copy number variation genetic marker. The variety identification of spotted tumor cattle is realized according to a CNV section which contains about 490Kb of a KIT gene and is subjected to repeated mutation on a cattle chromosome 6 found by carrying out whole genome CNV analysis on spotted hair color character tumor cattle and other pure color hair color character cattle varieties. According to the CNV segment, molecular marker-assisted selection can be carried out on tumor cattle hair color characters on the DNA level, so that a population with excellent genetic resources is rapidly established.
Owner:NORTHWEST A & F UNIV +1

Gastrointestinal stromal tumor drug-resistant cell model as well as construction method and application thereof

The invention relates to a gastrointestinal stromal tumor drug-resistant cell model as well as a construction method and application thereof. In-vitro induction is performed on gastrointestinal stromal tumor cell strains GIST882 by an intermittent concentration gradient multiplication method, a drug-resistant cell line with Sunitinib IC50 of 127.5 + / -2.15 [mu] mol / L and a drug resistance index of5.30 is established, the cell line changes in cell morphology, ultrastructure and cell cycles, Kit gene sequencing shows that 17 exon R804L mutation exists, INPP4B gene sequencing shows that 18 exon V466I mutation exists, and besides, IRS2, SOX9, FLCN, WISP3, SOX10 and MYCN also have mutation difference. The invention lays a foundation for further researching the drug resistance mechanism of the Sunitinib and screening new drugs for treating gastrointestinal stromal tumors.
Owner:SHANGHAI PUTUO DISTRICT LIQUN HOSPITAL

Assay kit and method for testing CYP1B1 gene polymorphism through pyrosequencing method

The invention discloses an assay kit and a method for testing CYP1B1 gene polymorphism through a pyrosequencing method. The assay kit is used for testing the CYP1B1 gene polymorphism, specifically rs1056836(C) G mononucleotide polymorphism. The assay kit comprises a primer, such as SEQIDN.2-4. The assay kit can be used for testing the CYP1B1 gene polymorphism in an accurate, quick and high flux manner, and thus, the individualized drug administration of substrate anti-microtubule like chemotherapeutics and the like can be realized safely, reasonably, effectively.
Owner:周宏灏

Genetic testing for improved cattle fertility

ActiveUS20160237508A1Preserve viabilityAnimal reproductionMicrobiological testing/measurementBiotechnologyBone Morphogenetic Protein Gene
Arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on novel SNPs on genes of the bovine transforming growth factor-β (TGF-β) signaling pathway for improved bovine fertilization rate. The methods and compositions of the present invention are related to SNPs in the DNA-binding protein inhibitor 3 (ID3) gene, and in the bone morphogenetic protein 4 (BMP4) gene corresponding to position 2702 of SEQ ID NO: 2. Also disclosed are methods for determining viability of developing bovine embryos by measuring the expression level of one or more target genes in the TGF-signaling pathway, and selecting for implantation only embryos whose target gene expression level is not up-regulated.
Owner:WISCONSIN ALUMNI RES FOUND
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