56 results about "Kit gene" patented technology

The invention discloses a method for breeding kidding traits by utilizing 3-gene pyramiding effect. The method comprises the following steps: amplifying a 3' untranslated region of a stem cell factor (SCF) gene, an exon 7 of a III type transmembrane tyrosine kinase receptor (KIT) gene and an intron 1 of a kiss (KISS1) gene respectively by using three pairs of primers and by taking goat genome DNA as a template; judging the size of each amplification product by using agarose gel electrophoresis at the concentration of 1.5%; screening the site mutation of the amplification products of the three pairs of primers by a DNA sequencing technology; performing genetic typing and gene frequency analysis on the single nucleotide polymorphisms (SNPs) of three sites of the SCF gene, the KIT gene and the KISS1 gene by using polyacrylamide gel electrophoresis at the concentration of 10% and agarose gel electrophoresis at the concentration of 3.5%; analyzing the relationship between different genotype combinations and kidding quantity; and screening out the optimal genotype combination.

The invention discloses a C-KIT gene multipoint mutation single tube fast detection method and kit. The C-KIT gene multipoint mutation single tube fast detection method comprises 1, designing ARMS primers and a corresponding downstream primer, intermediary connection primer, general fluorescent probe and quenching probe, 2, mixing the ARMS primers, the corresponding downstream primer, intermediary connection primer, general fluorescent probe and quenching probe and a hot-start fast Taq enzyme system and carrying out amplification, and 3, detecting a fluorescence change of the reaction system to determine if point mutation exists. The detection method can be operated simply and can realize qualitative detection of multipoint mutation existence. The detection method utilizes the primers with low design difficulty, greatly reduces a primer synthesis cost, solves limitation of the existing ARMS technology, greatly improves detection throughput, realizes single tube reaction replacing the existing multi-tube detection and saves manpower and material resources.

Gene chip for inspecting HIV to reverse transcriptase inhibitor resistance and reagent kit are disclosed. The gene chip consists of solid-phase carrier and oligonucleotide probe fixed on the solid-phase carrier, which comprises mutation site DNA fragment selected in HIV reversed transcriptive enzyme gene area, PCR amplifying for sample cDNA to be inspected by primer, purification marking for amplified product and hybridizing with the gene chip, acquiring hybridized signal by gene chip scanner, analyzing and appraising to determine medicine-tolerance site mutation. It's simple, accurate and repeated.

The invention relates to a nucleotide specific to wzt of Legionella pneumophila serogroup 1 and application thereof. The nucleotide is a nucleotide shown as SEQ ID NO: 1 and/or a nucleotide shown as SEQ ID NO: 2 and is complementary with the nucleotides. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Legionella pneumophila serogroup 1, a gene chip or a micro-array. The nucleotide specific to the wzt of the Legionella pneumophila serogroup 1 provided by the invention as well as the PCR kit, the gene chip or the micro-array including the nucleotide have the advantages of strong practical applicability, simple preparation method of the PCR kit, short detection period, high speed, strong maneuverability, easiness for industrial production, low detection cost, high accuracy and high sensitivity.

The invention discloses a c. A method for detecting mutation of kit gene comprising the follow steps: S1, according to 9, 11, 13 and 17 c-Kit Exon DNA Sequences, designing Specific Amplification Primers; S2, PCR amplification of No. 9, 11, 13 and 17 c by using the specific amplification primers designed in step S1; Kit exon, and the PCR amplified products being digested by enzymes; S3, carrying out cyclic amplification on that enzymatic hydrolysate according to the sequence primer to obtain a Sanger fragment; S4, using the Sanger fragment obtained in the step S3, analyzing No.9, 11, 13 and 17c-Kit exon DNA sequence information through an automatic gene instrument, comparing with wild genotype to find out the mutation site. The invention designs the specific amplification primer, shortensthe length of the primer, improves the specific amplification sensitivity, and intuitively sees whether the target gene amplified by PCR is qualified through the specific amplification primer, therebydeciding whether to carry out the next step and avoiding wasting the reagent.

Gene chip for inspecting and parting SARS coronavirus, its method and reagent kit are disclosed. The gene chip consists of solid-phase carrier and oligonucleotide probe fixed on the solid-phase carrier, which comprises DNA fragment containing S gene mutation site selected from S gene sequence with one or multiple SARS coronavirus and DNA fragment selected from S gene conservative sequence of SARS coronavirus. The process is carried out by PCR amplifying for sample cDNA to be inspected, hybridizing with the gene chip, determining nucleotide kind by different fluorescent value and parting for SARS sample to be inspected. It's simple, accurate and repeated.

The invention relates to a nucleotide specific to hafinia alvei G5907, G5908, G5913 and G5916, and an application of the nucleotide. The nucleotide comprises one of nucleotides shown in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR kit and a gene chip for detecting hafinia alvei. The invention provides the nucleotide specific to hafinia alvei G5907, G5908, G5913 and G5916, as well as the PCR kit and the gene chip comprising the nucleotide. The technology has the advantages of simple and convenient operation method, short detection cycle, low detection cost, high accuracy and high sensitivity, and is suitable for industrial production.

The invention provides primers for detecting c-kit gene mutation, and the primers include a forward primer and a backward primer for exon 8 and a forward primer and a backward primer for exon 17. The invention belongs to the technical field of biological detection, and the primers provided by the invention can specifically detect c-kit gene exons 8 and 17 mutations, and the specificity of the primers is good, and the accuracy is high, and the efficiency of the detection is increased.

The invention provides sgRNA for specific recognition of a pig KIT gene, a coding DNA thereof and a kit, and application thereof, belonging to the technical field of gene engineering. In the sgRNA, anucleotide sequence responsible for identifying a target fragment region is a sequence as shown in SEQ ID NO.1, a sequence as shown in SEQ ID NO.2 or a sequence as shown in SEQ ID NO.3; and the sgRNAenables a CRISPR/Cas9 gene editing system to delete redundant mutation copies of the KIT gene on a pig genome, so the KIT gene can normally express KIT protein with biological activity, and the hair color of a pig is changed.

The invention relates to nucleotides specific to Hafnia alveibifermentans G58097, G5898 and G5900 and their application. The nucleotides include one type of nucleotides shown in SEQ ID NO: 1 to 6. These nucleotides are applicable to the preparation of PCR kits and gene chips used for detecting Hafnia alveibifermentans. The invention relates to the nucleotides specific to Hafnia alveibifermentans G58097, G5898 and G5900, and a PCR kit and a gene chip both including the nucleotides; an operating method is simple, a detection period is short, detection cost is lower, accuracy is high, sensitivity is high, and the nucleotides, the PCR kit and the gene chip are easy to industrially produce.

The invention discloses a molecular marker for the diagnosis and prognosis judgment of a canine breast tumor. The molecular marker is an ICAM1 gene with a nucleotide sequence shown in the formula of SEQ ID NO: 1. The invention also discloses RT-qPCR detection primers for detecting ICAM1 gene expression. The primers have nucleotide sequences shown in the formulas of SEQ ID NO: 2-3. The invention also discloses a kit for diagnosis and prognosis determination of the canine breast tumor. The ICAM1 gene only is significantly low expressed in the malignant tumor group. The expression level of the ICAM1 gene in canine mammary gland tumor cells isolated from metastatic lesions is significantly different from that of primary tumor cells so that it is proved that the ICAM1 can identify benign and malignant canine breast tumors and is participated in the infiltration and metastasis of canine mammary tumors. Therefore, ICAM1 can be used as a molecular marker for the diagnosis and prognosis of canine breast tumors. The molecular marker related to canine mammary gland tumor metastasis has high sensitivity and specificity, can identify the nature of the canine breast tumor, can evaluate the prognosis, can determine the treatment plan in time, and can improve the survival rate of the diseased dog.

The invention discloses a method for identifying a spotted tumor cattle variety by using a KIT gene copy number variation genetic marker. The variety identification of spotted tumor cattle is realized according to a CNV section which contains about 490Kb of a KIT gene and is subjected to repeated mutation on a cattle chromosome 6 found by carrying out whole genome CNV analysis on spotted hair color character tumor cattle and other pure color hair color character cattle varieties. According to the CNV segment, molecular marker-assisted selection can be carried out on tumor cattle hair color characters on the DNA level, so that a population with excellent genetic resources is rapidly established.

The invention relates to a gastrointestinal stromal tumor drug-resistant cell model as well as a construction method and application thereof. In-vitro induction is performed on gastrointestinal stromal tumor cell strains GIST882 by an intermittent concentration gradient multiplication method, a drug-resistant cell line with Sunitinib IC50 of 127.5 +/-2.15 [mu] mol/L and a drug resistance index of5.30 is established, the cell line changes in cell morphology, ultrastructure and cell cycles, Kit gene sequencing shows that 17 exon R804L mutation exists, INPP4B gene sequencing shows that 18 exon V466I mutation exists, and besides, IRS2, SOX9, FLCN, WISP3, SOX10 and MYCN also have mutation difference. The invention lays a foundation for further researching the drug resistance mechanism of the Sunitinib and screening new drugs for treating gastrointestinal stromal tumors.

The invention discloses an assay kit and a method for testing CYP1B1 gene polymorphism through a pyrosequencing method. The assay kit is used for testing the CYP1B1 gene polymorphism, specifically rs1056836(C) G mononucleotide polymorphism. The assay kit comprises a primer, such as SEQIDN.2-4. The assay kit can be used for testing the CYP1B1 gene polymorphism in an accurate, quick and high flux manner, and thus, the individualized drug administration of substrate anti-microtubule like chemotherapeutics and the like can be realized safely, reasonably, effectively.

Arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on novel SNPs on genes of the bovine transforming growth factor-β (TGF-β) signaling pathway for improved bovine fertilization rate. The methods and compositions of the present invention are related to SNPs in the DNA-binding protein inhibitor 3 (ID3) gene, and in the bone morphogenetic protein 4 (BMP4) gene corresponding to position 2702 of SEQ ID NO: 2. Also disclosed are methods for determining viability of developing bovine embryos by measuring the expression level of one or more target genes in the TGF-signaling pathway, and selecting for implantation only embryos whose target gene expression level is not up-regulated.

The invention relates to a nucleotide specific to hafinia alvei G5902, G5903, G5904 and G5906, and an application of the nucleotide. The nucleotide comprises one of nucleotides shown in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR kit and a gene chip for detecting hafinia alvei. The invention provides the nucleotide specific to hafinia alvei G5902, G5903, G5904 and G5906, as well as the PCR kit and the gene chip comprising the nucleotide. The technology has the advantages of simple and convenient operation method, short detection cycle, low detection cost, high accuracy and high sensitivity, and is suitable for industrial production.