38 results about "O-Antigens" patented technology

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention discloses a vibrio parahaemolyticus bacteriophage vB_VpP_DE17, which has a strong lysis effect on vibrio parahaemolyticus and can infect 10 vibrio parahaemolyticus in 11 O antigens. The bacteriophage can be amplified through a specific host to generate a fermentation product with high titer, and can tolerate the high temperature of 60 DEG C and tolerate pH of 5.0-10.0. The optimal multiplicity of infection (MOI) of the vibrio parahaemolyticus bacteriophage is wide, and the vibrio parahaemolyticus bacteriophage has a strong effect of preventing and controlling the vibrio parahaemolyticus when the MOI is in a range of 1-0.1. Therefore, the vibrio parahaemolyticus bacteriophage can be used for preventing and controlling acute hepatopancreas necrosis syndrome caused by vibrio parahaemolyticus in the aquaculture process, and has high application value.

The present disclosure generally relates to genetic engineering of bacteria. More particularly, the present disclosure describes genetic engineering of E. coli to create mutant O-antigen ligase, as well as novel lipopolysaccharide molecules resulting from that genetic engineering. Methods for using those novel molecules are also described.

The invention provides a nucleotide specific for Escherichia coli 0123 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 17084 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases, and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia 0123.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15253 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustaining the functions of the isolated nucleotides, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Shigella dysenteriae 10. The invention proves the high specifity of oligonucleotides for Shigella dysenteriae 10 O-antigen through PCR. A method for detecting and identifying Shigella dysenteriae 10 by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 054 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 054, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 14062 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the Escherichia 054, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes. The invention proves the high specifity of oligonucleotides for Escherichia 054 O-antigen through PCR. A method for detecting and identifying Escherichia 054 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for Escherichia coli 0151 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia 0151, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 15670 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0151. The invention proves the high specifity of oligonucleotides for Escherichia 0151 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0151 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention provides a nucleotide specific for shigella bodyii 10 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in shigella bodyii 10, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 13402 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of wzx gines in the O-antigen gene cluster originating from the shigella bodyii 10, and genes and wzy genes having similar functions with wzx, and genes having similar functions with wzy genes or wzy genes or orf 8 genes with unknown functions. The invention proves the high specifity of oligonucleotides for shigella bodyii 10 O-antigen through PCR. A method for detecting and identifying shigella bodyii 10 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.

The invention relates to specific nucleotides for Vibrio cholerae O-antigens and applications of the specific nucleotides. The nucleotides comprise 1), at least one of nucleotides represented in SEQ ID NO: 1-8; 2), at least one of nucleotides complemented with the nucleotides represented in SEQ ID NO:1-8. The nucleotides can be used for preparing a PCR (polymerase chain reaction) kit and a gene chip for detecting Vibrio cholerae. The specific nucleotides for Vibrio cholerae O-antigens as well as the PCR kit and the gene chip containing the nucleotides have high practicability, a preparation method of the PCR kit is simple and convenient, the detection period is short, the speed is high, the operability is high, industrial production is facilitated, and the detection cost is relatively lower; the accuracy and sensitivity are high.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention relates to a specific nucleotide for aeromonas hydrophila O antigens and application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8; (2) at least one of nucleotides which are complementary with the nucleotides shown as SEQ ID NO:1 to SEQ ID NO:8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit and a gene chip, which are used for detecting aeromonas hydrophila. The specific nucleotide for theaeromonas hydrophila O antigens and the PCR kit and the gene chip, which contain the nucleotide, have high practicability; the PCR kit has the advantages of simple and convenient preparation method, short detection period, rapid speed and high operability; the industrial production is facilitated and the detection cost is relatively lower; the accuracy is high and the sensitivity is high.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.

The invention relates to a nucleotide specific to Aeromonas hydrophila O antigens and an application thereof. The nucleotide comprises: (1) at least one of nucleotides shown as SEQ ID NO:1 to 8; and (2) at least one of nucleotides being complementary with the nucleotides shown as SEQ ID NO:1 to 8. The nucleotides can be used for preparing a PCR (Polymerase Chain Reaction) kit for detecting Aeromonas hydrophila and a gene chip. The nucleotide specific to the Aeromonas hydrophila O antigens and the PCR kit and the gene chip including the nucleotide have high practicability. The PCR kit has the advantages of easiness and convenience in preparation, short detection period, high speed, high operability, easiness for industrial production, relatively low detection cost, high accuracy and high sensitivity.

The invention provides a nucleotide specific for Escherichia coli 0159 O-antigen, which is the total nucleotide sequence of the gene cluster for controlling the synthesis of O-antigens in Escherichia coli 0159, e.g. isolated nucleotide represented by SEQ ID No:1, with overall length of 13749 bases, or nucleotide of SEQ ID No:1 including one or more inserted, deleted or substituted bases and sustains the isolated nucleotide functions, it also includes the oligonucleotides of glycosyl transferase genes and oligosaccharide unit treatment genes in the O-antigen gene cluster originated from Escherichia coli 0159. The invention proves the high specifity of oligonucleotides for Escherichia coli 0159 O-antigen through PCR. A method for detecting and identifying Escherichia coli 0159 in human body and the environment by means of the oligonucleotide according to the invention is also disclosed.