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Method for breeding kidding traits by utilizing 3-gene pyramiding effect

A technology of gene aggregation and goat, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms.

Inactive Publication Date: 2014-01-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

After injecting kisspeptins into the ventricle and peripheral blood of adult male rats, it was found that the secretion of FSH, LH and LHRH increased significantly, but the time required for the stimulation effect was different

Method used

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  • Method for breeding kidding traits by utilizing 3-gene pyramiding effect
  • Method for breeding kidding traits by utilizing 3-gene pyramiding effect
  • Method for breeding kidding traits by utilizing 3-gene pyramiding effect

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Embodiment Construction

[0048] The following is the 3' untranslated region of stem cell factor (SCF) amplified by primers P1, P2 and P3 through the extraction, detection and concentration analysis of goat genomic DNA, and type III transmembrane casein Specific examples of polyacrylic gel and agarose gel electrophoresis analysis of exon 7 of amino acid kinase receptor (KIT) and intron 1 of kissin (KISS1).

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Abstract

The invention discloses a method for breeding kidding traits by utilizing 3-gene pyramiding effect. The method comprises the following steps: amplifying a 3' untranslated region of a stem cell factor (SCF) gene, an exon 7 of a III type transmembrane tyrosine kinase receptor (KIT) gene and an intron 1 of a kiss (KISS1) gene respectively by using three pairs of primers and by taking goat genome DNA as a template; judging the size of each amplification product by using agarose gel electrophoresis at the concentration of 1.5%; screening the site mutation of the amplification products of the three pairs of primers by a DNA sequencing technology; performing genetic typing and gene frequency analysis on the single nucleotide polymorphisms (SNPs) of three sites of the SCF gene, the KIT gene and the KISS1 gene by using polyacrylamide gel electrophoresis at the concentration of 10% and agarose gel electrophoresis at the concentration of 3.5%; analyzing the relationship between different genotype combinations and kidding quantity; and screening out the optimal genotype combination.

Description

technical field [0001] The invention belongs to the field of molecular genetics and breeding, and specifically relates to a method for breeding lambing traits of goats by utilizing the aggregation effect of 3 genes. The method uses PCR-RFLP and DNA sequencing technology to detect the 3' of the stem cell factor (SCF) gene of the individual being examined. Single-strand DNA base mutation polymorphisms in the untranslated region, type III transmembrane tyrosine kinase receptor (KIT) gene exon 7 and kissin (KISS1) gene intron 1, by analyzing different genotype combinations The relationship between the number of goats and the number of lambs, screen out the best genotype combination, analyze the matching methods of high-yield individuals with the best genotype combination, and then apply these matching methods to form a core group of multi-gene aggregated fine breeds of goats with multiple lambs . Background technique [0002] The low lambing rate is the main bottleneck restrict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 曹斌云安小鹏侯金星王建刚宋宇轩
Owner NORTHWEST A & F UNIV
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